2 research outputs found
Examination of Genetic Diversity of Common Bean from the Western Balkans
In this study, genetic diversity of 119 accessions of common bean (Phaseolus vulgaris) from five former Yugoslav republics constituting the western Balkans was assessed by 13 microsatellite markers. This set of markers has proven before to efficiently distinguish between bean genotypes and assign them to either the Andean or the Mesoamerican gene pool of origin. In this study, 118 alleles were detected or 9.1 per locus on average. Four groups (i.e., Slovene, Croatian, Bosnian, and Serbian) showed similarly high levels of genetic diversity as estimated by the number of different alleles, number of effective alleles, Shannon's information index, and expected heterozygosity. Mildly narrower genetic diversity was identified within a group of Macedonian accessions; however, this germplasm yielded the highest number of private alleles. All five germplasms share a great portion of genetic diversity as indicated by the analysis of molecular variance (AMOVA). On the basis of the scored number of migrants, we concluded that the most intensive gene flow in the region exists in Bosnia and Herzegovina. Cluster analysis based on collected molecular data classified the accessions into two large clusters that corresponded to two gene pools of origin (i.e., Andean and Mesoamerican). We found that Andean genotypes are more prevalent than Mesoamerican in all studied countries, except Macedonia, where the two gene pools are represented evenly. This could indicate that common bean was introduced into the western Balkans mainly from the Mediterranean Basin. Bayesian cluster analysis revealed that in the area studied an additional variation exists which is related to the Andean gene pool. Different scenarios of the origin of this variation are discussed in the article
Genetic diversity of common bean accessions from former Yugoslav Republic of Macedonia as revealed by molecular and morphological markers
Cultivation of common bean has a long tradition in the Former Yugoslav
Republic of Macedonia (FYROM) and is still nowadays important part of the
human diet. In a study reported here 71 accessions from the FYROM were
assessed for genetic diversity with the aim to provide information on genetic
structure of Macedonian common bean germplasm and to depict its
peculiarities. A total of 71 accessions were assessed using 13 microsatellite
and 16 morphological markers. The average number of alleles per
microsatellite was 5.8, and ranged from three to 16 alleles. High capacity of
selected markers for distinguishing genotypes was identified by the
calculation of a very low value of probability of identity. The relationship
among 71 studied accessions was assessed by hierarchical cluster analysis. A
very clear separation of accessions into two groups was observed in the UPGMA
dendrogram. The larger represented Andean gene pool and contained 40
accessions (56% of total), while the other 31 accessions (44% of total)
composed Mesoamerican gene pool. The two groups were successfully
discriminated by eight morphological traits. Within the larger Andean cluster
in the UPGMA dendrogram a sub-group of 16 climbing accessions was separated
from 24 bush accessions. The absence of the string in the pods of the
climbers suggests that this sub-group comprises snap beans grown primarily
for their fresh pods. There were eight morphological traits in total that
distinguished the two Andean sub-groups. Assessment of genetic relationship
among accessions, their classification into respective gene pool and
identification of morphological peculiarities provided valuable information
for the management of plant gene bank and Macedonian bean breeding program