Exploration of Regional Agrowastes for the Production of Pectinase by Aspergillus niger

Svrha je ovog istraživanja procjena lokalno dostupnog poljoprivrednog otpada bogatog pektinom, i to korice limuna, stabljike sirka i glavice suncokreta, kao supstrata za proizvodnju pektinaze submerznim uzgojem plijesni Aspergillus niger DMF 27 i površinskim uzgojem plijesni A. niger DMF 45 na čvrstoj podlozi. Maksimalna količina endopektinaze (4,8 U/g) i egzopektinaze (17,2 U/g) dobivena je površinskim uzgojem na čvrstoj podlozi od glavica suncokreta, a nešto manje (endopektinaze 2,0 i egzopektinaze 10,2 U/g) na podlozi od korice limuna. Dodatkom izvora ugljika i dušika poljoprivrednom otpadu povećana je proizvodnja pektinaze, pri čemu je dodatak saharoze imao veći učinak od dodatka glukoze pri uzgoju na čvrstoj podlozi. Međutim, pri submerznom uzgoju dodatak glukoze povećao je prinos pektinaze. Amonijev sulfat kao izvor dušika povećao je proizvodnju pektinaze u oba postupka uzgoja.The aim of this study was to evaluate locally available pectin rich agrowastes, viz. lemon peel, sorghum stem and sunflower head, as substrates for the production of pectinase by Aspergillus niger DMF 27 and A. niger DMF 45 in submerged fermentation (SmF) and solid-state fermentation (SSF) systems, respectively. The maximum amount of endo- (4.8 U/g) and exopectinases (17.2 U/g) was obtained from sunflower head followed by lemon peel (endopectinase 2.0 and exopectinase 10.2 U/g) in solid-state system. The increased level in the production of pectinases was noticed when the agrowastes were supplemented with additional carbon and nitrogen sources, and supplementation of sucrose was more effective than glucose in SSF. But, glucose yielded more pectinases in SmF. Among the nitrogen sources, ammonium sulphate raised the production level of pectinases from all the substrates in both SmF and SSF systems