Historical urban planning analysis of the Kama and Toima riverside areas (on the example of the city of Elabuga in the 19TH -20TH century)

In cities on large rivers, increasing attention is being given to coastal areas as the basis for their sustainable development. At the same time, most of the development proposals are too radical and require significant financial investments that are not available in small cities. To make the right decisions, a historical urban-planning analysis is required to identify the reasons for the decline of coastal areas, and further arguments are needed in support of decisions for their transformation. This study is an attempt to verify the statement that the sustainable development of coastal areas should take into account the previous evolutionary trends, and their development needs to be monitored regularly in order to make timely adjustments to their functional zoning. Using the example of the old city of Elabuga located on two rivers, Kama and Toima, the main reasons influencing its zoning are highlighted. Depending on momentary interest in a particular function, it may either evolve and flourish or fall in decline, but is never completely driven out. Based on the analysis, a potential path for the development of coastal areas is identified by raising social interest and demand for historical functions through their renovation, additions, and improvement of accessibility

Influence of the Dielectric Coating of the Outer Side of the Cathode in the Anode–Cathode Pairs of a Molecular Electronic Sensitive Element on the Conversion Coefficient

Molecular electronic sensors of motion parameters use miniature electrochemical cells as a sensitive element, in which the interelectrode current is sensitive to external mechanical influences. New approaches for creating conversion elements are based on precision methods of micromachining materials. The use of new technologies has opened up the possibility of creating sensitive elements with configurations that have not been previously studied, and for which there is no clear understanding of the regularities that determine the output parameters depending on the geometry of the conversion elements. This work studies the influence of the dielectric coating on the surface of the cathodes on the conversion coefficient. The transforming structure has been made from three plates. The outer plates were an anode–cathode electrode pair. The middle plate served as a separator between the pairs of electrodes. It was found that an insulating layer on the side of the cathode facing away from the adjacent anode allows the conversion factor to be doubled. This result is applicable for a wide class of conversion elements made with microelectronic technologies, as well as structures made of mesh electrodes

Holocene Paleoenvironmental Implications of Diatom, Non-Pollen Palynomorph, and Organic Carbon Records from the Kandalaksha Bay of the White Sea (European Arctic)

Variations in sea surface conditions and sea level through the Holocene in the Kandalaksha Bay, the White Sea, were reconstructed based on the study of core sediments from the outer Kandalaksha Bay, using the modern analog technique applied to dinocysts in addition to diatoms, TOC, δ13Corg, CaCO3, and grain size data. The chronostratigraphy of the core sediments was defined from accelerator mass spectrometry 14C dates on mollusk shells. The results indicated an increase in water depth in the outer Kandalaksha Bay and in the central Dvina Bay until the late Holocene. From about 9.5 to 7.5 cal kyr BP, the data suggested a general trend of increasing sea surface temperatures (up to 14 °C), at least in areas with inflow of Atlantic waters. The last 2.5 kyr were characterized by increased freshwater runoff to the White Sea

Diatom and Dinocyst Production, Composition and Flux from the Annual Cycle Sediment Trap Study in the Barents Sea

This paper presents the diatom and palynomorph data from a sediment trap deployed in the northern part of the East Barents Sea for an annual cycle from August 2017 to August 2018. The average monthly fluxes of diatoms and dinoflagellate cysts in the photic layer of the northeastern part of the Barents Sea varies from 10.4 × 103 to 640.8 × 103 valves m−2 day−1 and from 0.3 × 103 to 90.0 × 103 cysts m−2 day−1, respectively. Their fluxes are related to the low irradiance of the photic layer during the sea-ice cover period, dominance of southward currents, modern climate, and nepheloid layer conditions. Based on redundancy analysis of the relationship between the fluxes of diatoms and dinoflagellate cysts and organic carbon fluxes, sea-ice covers, and the seasonal cycle of light availability we determined the following. First, sea-ice-associated diatoms and dinocysts are exported to the sediment trap from the melting sea ice with a two-week delay. Second, the appearance of freshwater diatoms and green algae in the sinking material accumulating from March 2018 to July 2018 is also related to the melting of sea ice. And third, the presence of Coscinodiscus radiatus, C. perforatus, Shionodiscus oestrupii and Operculodinium centrocarpum in the diatoms and dinocysts species composition throughout the year indicates the advection of Atlantic waters into the Barents Sea up to 80° N

Protection Activity of 1,4-Naphthoquinones in Rotenone-Induced Models of Neurotoxicity

The MTS cell viability test was used to screen a mini library of natural and synthetic 1,4-naphthoquinone derivatives (1,4-NQs) from marine sources. This screening identified two highly effective compounds, U-443 and U-573, which showed potential in protecting Neuro-2a neuroblastoma cells from the toxic effects of rotenone in an in vitro model of neurotoxicity. The selected 1,4-NQs demonstrated the capability to reduce oxidative stress by decreasing the levels of reactive oxygen species (ROS) and nitric oxide (NO) in Neuro-2a neuroblastoma cells and RAW 264.7 macrophage cells and displayed significant antioxidant properties in mouse brain homogenate. Normal mitochondrial function was restored and the mitochondrial membrane potential was also regained by 1,4-NQs after exposure to neurotoxins. Furthermore, at low concentrations, these compounds were found to significantly reduce levels of proinflammatory cytokines TNF and IL-1β and notably inhibit the activity of cyclooxygenase-2 (COX-2) in RAW 264.7 macrophages. The results of docking studies showed that the 1,4-NQs were bound to the active site of COX-2, analogically to a known inhibitor of this enzyme, SC-558. Both substances significantly improved the behavioral changes in female CD1 mice with rotenone-induced early stage of Parkinson’s disease (PD) in vivo. It is proposed that the 1,4-NQs, U-443 and U-573, can protect neurons and microglia through their potent anti-ROS and anti-inflammatory activities

Holothurian triterpene glycoside cucumarioside A2-2 induces macrophages activation and polarization in cancer immunotherapy

Abstract Background Despite intensive developments of adoptive T cell and NK cell therapies, the efficacy against solid tumors remains elusive. Our study demonstrates that macrophage-based cell therapy could be a potent therapeutic option against solid tumors. Methods To this end, we determine the effect of a natural triterpene glycoside, cucumarioside A2-2 (CA2-2), on the polarization of mouse macrophages into the M1 phenotype, and explore the antitumor activity of the polarized macrophage. The polarization of CA2-2-pretreated macrophages was analyzed by flow cytometry and confocal imaging. The anti-cancer activity of CA2-2 macrophages was evaluated against 4T1 breast cancer cells and EAC cells in vitro and syngeneic mouse model in vivo. Results Incubation of murine macrophages with CA2-2 led to polarization into the M1 phenotype, and the CA2-2-pretreated macrophages could selectively target and kill various types of cancer in vitro. Notably, loading near-infrared (NIR) fluorochrome-labeled nanoparticles, MnMEIO-mPEG-CyTE777, into macrophages substantiated that M1 macrophages can target and penetrate tumor tissues in vivo efficiently. Conclusion In this study, CA2-2-polarized M1 macrophages significantly attenuated tumor growth and prolonged mice survival in the syngeneic mouse models. Therefore, ex vivo CA2-2 activation of mouse macrophages can serve as a useful model for subsequent antitumor cellular immunotherapy developments