Seroprevalence of Hepatitis-B virus and Hepatitis-C virus in pregnant women in Hyderabad.

Introduction: Infections of the hepatitis B virus (HBV) and the hepatitis C virus (HCV) are major global public health problem. Approximately 350 million and 190 million people worldwide are infected with HBV and HCV respectively.  Objective: To evaluate seroprevalence of hepatitis B and C among pregnant  women of Hyderabad. Methodology: This prospective cross sectional study conducted at Pathology department of a tertiary care hospital of Hyderabad during Jan 2019 to July 2019. During period of study data of 500 pregnant women recorded on pre structured proforma after taking informed consent. Causes of hepatitis other than B or C virus were excluded. Status of HCV and HBV was confirmed using Elisa method. Results: showed 7% seropositivity for HCV and 4.2% for HBV among pregnant women. Conclusion: Among pregnant females, in Hyderabad  there is a higher seropositivity of anti HCV antibodies as compared to HBsAg. Both seropositivity to HBV and HCV was found to be more prevalent in the 30-39 year age group. Keywords: Pregnant women, Hyderabad, Seroprevalence HCV, Seroprevalence HBV. &nbsp

The frequency of vibrio cholera 01 EL TOR (Inaba And Ogawa) and its Resistance Pattern in Karachi.

Introduction: Cholera is an acute infectious disease of small intestine, caused by the bacterium Vibrio cholerae. More than 200 serogroups of V. cholerae have been identified. In Iran, Inaba strains were 75% resistant against trimethoprim-sulfamethoxazole in 2011, while during 2012-13 it was 100%. Ogawa strains showed 96% resistance against trimethoprim-sulfamethoxazole in 2011, whereas 100% resistant in 2012, while no resistance was seen in 2013 against the same antibiotic.   Objective: To determine the frequency of vibrio cholera 01 EL TOR (Inaba And Ogawa) and its resistance pattern in Karachi. Methodology: Samples were collected from patients with acute diarrhea with rice-water stool from Medical Unit, JPMC and NICH, Karachi between October 2015 to August 2016. These samples were then transported to Microbiology Department, Basic Medical Sciences Institute, JPMC, Karachi and processed according to standard protocol. Results: No growth was noticed in 147 (66.81%) samples while a total of 28 (12.7%) were V. cholerae species, and 45 (20.45%) were other organisms. The distribution of V. cholerae serotypes, out of 28 V. cholerae species, 13 (46.4%) were of Inaba serotypes, 09 (32.1%) were of Ogawa serotypes while 06 (21.4%) were of Non-01 serotypes. Conclusion: The susceptibility results of tetracycline and trimethoprim-sulfamethoxazole are not very favorable. Involvement of multidrug resistant V. cholerae O1 serotypes in the community is a very serious public health concern. Such patients were observed to be very difficult to treat in the community. &nbsp

An In-Vitro Evaluation of Microleakage in Resin-Based Restorative Materials at Different Time Intervals

A vital feature of conservative dentistry is the adhesion of the restorative material to the tooth structure for restoration of the tooth substance lost due to dental decay, trauma, or dental imperfections. In a perfect world, a restorative material should generate a lasting adhesion by bonding the restoration with tooth tissues. The ingress of micro-organisms, oral fluids, molecules, and ions through microscopic spaces due to faulty adhesion between restoration and tooth structure is known as microleakage. This study is focuses on the evaluation of adhesive failures between the restorative materials. In the past, studies have focused more on the bonding potential of a restorative material with the tooth surface. Therefore, there is need to carry out a study that compares the microleakage between resin-based restorative materials in a sandwich manner with and without the intermediate bonding layer after immersion in 2% methylene blue dye at different time intervals. The restorative materials used were composite Ceram X Mono plus (DENTSPLY) and Z350 (3M ESPE), Vitremer resin modified glass ionomer cement (RMGIC) (3M ESPE), smart dentine replacement SDR (3M ESPE), Bond NT (DENTSPLY), and Universal Bond (3M ESPE). A light emitting diode (LED) was used to cure the specimens. Artificial saliva was used as a storage medium for the specimens. Thermocycling of specimens was carried out at 500 cycles/30 s and 1000 cycles/30 s. The world health organization (WHO) grading tool for microleakage was used to analyze fluid ingress in the specimens through disclosing by 2% methylene blue dye. The statistical analysis was carried out with one-way analysis of variance (ANOVA) and Tukey post hoc test, keeping the level of significance at p ≤ 0.05. In Grade 0 = 85 samples, Grade 1 = 10 samples, Grade 2 = 7 samples, Grade 3 = 16 samples, and in Grade 4 = 2 samples were identified. This study describes that no microleakage was observed in SDR and resin composite groups as compared to Vitremer and resin composite groups