Comparison of Mean Lipid Profile in Calcific Aortic Stenosis Cases and Controls

Objective: This study was aimed to compare the mean lipid profile in calcific aortic stenosis patients and control group. Patients and Methods: Total 202 individuals, 101 cases and 101 controls visiting National Institute of Cardiovascular Disease and various tertiary care hospitals of Karachi from January 2012 to December 2012 were included in this study. Calcific AS patients having the age of ≥60 years fulfilling the inclusion criteria were selected from echocardiography department and OPD from NICVD. Age and gender match controls were selected from OPD. Lipid profile was done in DDRRL, Ojha Campus. Lipid profile estimates were carried out on HITACHI 902 analyzer using photometry technique. Results: Nonparametric Mann-Whitney test showed increase level of Triglyceride in calcific AS patients. Conclusion: Calcific AS patients showed altered lipid profile as compare to control group so dyslipidemia may be involved in its pathogenesis

Leptin to Adiponectin ratio as Atherogenic Index in Ischemic Heart Disease Patients

Objective: To determine plasma adiponectin and leptin levels in subjects with and without ischemic heart disease (IHD) and to find the correlation between leptin, adiponectin to the number of coronary vessels disease-using angiography.Patients and Methods: This is a cross-sectional study conducted Ziauddin University. 80 subjects were recruited who were undergoing angiography. Height, weight, Waist and hip circumference were measured. BMI and WHR were calculated accordingly. Venous blood was drawn to measure adiponectin and leptin using ELISA.Results: Leptin to adiponectin ratio (LA ratio) was significantly increased in three and two vessel disease compared with single vessel and non-significant groups.Conclusion: The study shows that adiponectin decreases and leptin increases in multivessel disease. LA ratio has been found to correlate well in two and more than two vessel disease

The Apolipoprotein E Polymorphism And Dyslipidemia In Elderly Patients Of Calcific Aortic Stenosis: A Case Control study

Objective: This study aimed to investigate the impact of the Apo E polymorphisms on plasma lipid profile and to identify the polymorphism of the apo-E gene as genetic predictor of calcific AS in Pakistani population.   Methodology: This was a case control study conducted in Dow University of Health Sciences and National Institute of Cardiovascular Disease, Karachi. It included total of 100 individuals, 50 echocardiographically identified calcific AS cases and 50 age and gender matched controls. Apo E allele frequencies were computed, lipid profiles were estimated and Apo E gene polymorphism was identified by the techniques of polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).   Result: Apo E 2, 3, and 4 allele frequencies were 16%, 52%, and 32% in calcific AS cases, and 10%, 52%, 28% in controls respectively (p=0.622). Out of 50 cases, 18% presented with mild AS, 22% moderate AS and 60% lied in severe calcific AS. It was observed that levels of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein (LDL) were higher in Apo E4 allele as compared to other genes in both cases and control.   Conclusion: The findings of this study suggested that Apo E4 allele of Apo E gene is an impotent risk factors for dyslipidemia while Apo E4 allele is not associated with calcific AS contemplates distinctive genetic backgrounds of CAD and AS