Effect of radiant exposure and UV accelerated aging on physicochemical and mechanical properties of composite resins

Currently, there is no consensus in terms of defining the minimum radiant exposure values necessary for achieving adequate properties of composite resin. In addition, the long-term influence that radiant exposure has on the properties of composite resins is still questionable. Objective: The objective of this study was to evaluate the effect of radiant exposure and UV accelerated aging on the physico-chemical and mechanical properties of micro-hybrid and nanofilled composite resins. Material and Methods: A nanofilled (Filtek Supreme; 3M ESPE) and a micro-hybrid composite resin (Filtek Z250; 3M ESPE) were investigated under different radiant exposures (3.75, 9, and 24 J/cm2) and UV accelerated aging protocols (0, 500, 1000, and 1500 aging hours). The degree of conversion (DC), flexural strength (FS), modulus (M), water sorption (WS), and solubility (WL) were evaluated. The results obtained were analyzed using two-way ANOVA and Tukey’s test. Comparisons were performed using a significance level of α=0.05. Results: The DC, FS, and M were found to be significantly influenced by both radiant exposure and accelerated aging time. The DC and EM increased with radiant exposure in the no-aging group (0-hour aging) for both micro-hybrid and nanofilled composites, whereas no correlation was found after accelerated aging protocols. WS and WL of micro-hybrid and nanofilled composite resins were scarcely affected by radiant exposure (p>0.05), whereas they were significantly reduced by accelerated aging (p<0.001). Conclusions: Although increasing radiant exposure affected the degree of conversion and mechanical properties of micro-hybrid and nanofilled composites, no influence on the hydrolytic degradation of the material was observed. In contrast, UV accelerated aging affected both the physico-chemical and mechanical properties of the composites

Búsqueda de los virus del nilo occidental, de la encefalitis de san Luis y de la encefalitis equina venezolana en mosquitos de Paraguay – resultados preliminares.

El objetivo general del proyecto fue determinar la presencia de arbovirus encefalíticos (VEEV, WNV y SLEV) en poblaciones humanas y de mosquitos de Paraguay.CONACYT - Consejo Nacional de Ciencias y TecnologíaPROCIENCI

Búsqueda de virus encefalíticos (wnv, slev y veev) en sueros de pacientes con sospecha de síndrome febril agudo y trastornos neurológicos.

Los virus del Nilo Occidental (WNV), de la Encefalitis de San Luis (SLEV) y de la Encefalitis Equina Venezolana (VEEV) pueden producir enfermedades febriles agudas que podrían ser confundidas con el dengue u otras patologías; también pueden causar trastornos neurológicos graves. Estos virus todavía no han sido detectados en nuestro país, sin embargo, existen reportes de circulación de los mismos en países limítrofes. El Paraguay cuenta con las condiciones favorables para propiciar el mantenimiento del ciclo de transmisión de estos virus, por lo que se considera de suma importancia el estudio de ellos en pacientes con afecciones previamente mencionadas.CONACYT - Consejo Nacional de Ciencias y TecnologíaPROCIENCI

Demographic, clinical, and service-use characteristics related to the clinician’s recommendation to transition from child to adult mental health services

Purpose: The service configuration with distinct child and adolescent mental health services (CAMHS) and adult mental health services (AMHS) may be a barrier to continuity of care. Because of a lack of transition policy, CAMHS clinicians have to decide whether and when a young person should transition to AMHS. This study describes which characteristics are associated with the clinicians’ advice to continue treatment at AMHS. Methods: Demographic, family, clinical, treatment, and service-use characteristics of the MILESTONE cohort of 763 young people from 39 CAMHS in Europe were assessed using multi-informant and standardized assessment tools. Logistic mixed models were fitted to assess the relationship between these characteristics and clinicians’ transition recommendations. Results: Young people with higher clinician-rated severity of psychopathology scores, with self- and parent-reported need for ongoing treatment, with lower everyday functional skills and without self-reported psychotic experiences were more likely to be recommended to continue treatment. Among those who had been recommended to continue treatment, young people who used psychotropic medication, who had been in CAMHS for more than a year, and for whom appropriate AMHS were available were more likely to be recommended to continue treatment at AMHS. Young people whose parents indicated a need for ongoing treatment were more likely to be recommended to stay in CAMHS. Conclusion: Although the decision regarding continuity of treatment was mostly determined by a small set of clinical characteristics, the recommendation to continue treatment at AMHS was mostly affected by service-use related characteristics, such as the availability of appropriate services

Cohort profile : demographic and clinical characteristics of the MILESTONE longitudinal cohort of young people approaching the upper age limit of their child mental health care service in Europe

Purpose: The presence of distinct child and adolescent mental health services (CAMHS) and adult mental health services (AMHS) impacts continuity of mental health treatment for young people. However, we do not know the extent of discontinuity of care in Europe nor the effects of discontinuity on the mental health of young people. Current research is limited, as the majority of existing studies are retrospective, based on small samples or used non-standardised information from medical records. The MILESTONE prospective cohort study aims to examine associations between service use, mental health and other outcomes over 24 months, using information from self, parent and clinician reports. Participants: Seven hundred sixty-three young people from 39 CAMHS in 8 European countries, their parents and CAMHS clinicians who completed interviews and online questionnaires and were followed up for 2 years after reaching the upper age limit of the CAMHS they receive treatment at. Findings to date: This cohort profile describes the baseline characteristics of the MILESTONE cohort. The mental health of young people reaching the upper age limit of their CAMHS varied greatly in type and severity: 32.8% of young people reported clinical levels of self-reported problems and 18.6% were rated to be ‘markedly ill’, ‘severely ill’ or ‘among the most extremely ill’ by their clinician. Fifty-seven per cent of young people reported psychotropic medication use in the previous half year. Future plans: Analysis of longitudinal data from the MILESTONE cohort will be used to assess relationships between the demographic and clinical characteristics of young people reaching the upper age limit of their CAMHS and the type of care the young person uses over the next 2 years, such as whether the young person transitions to AMHS. At 2 years follow-up, the mental health outcomes of young people following different care pathways will be compared. Trial registration number: NCT03013595

Índice de valoración de hábitat para ríos de montaña y piedemonte

Índices de calidad de hábitat in stream (IVH) y calidad de bosques de ribera (QBRp) adaptados para su uso en la región Patagónica.Fil: Miserendino, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagónica. Universidad Nacional de la Patagonia "San Juan Bosco". Centro de Investigación Esquel de Montaña y Estepa Patagónica; ArgentinaFil: Macchi, Pablo Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación en Paleobiología y Geología; Argentina. Universidad Nacional de Río Negro; ArgentinaFil: Kutschker, Adriana Mabel. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; ArgentinaFil: Brand, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagónica. Universidad Nacional de la Patagonia "San Juan Bosco". Centro de Investigación Esquel de Montaña y Estepa Patagónica; ArgentinaFil: Papazian, Gabriela. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel; Argentin

Study of the Role of the Tyrosine Kinase Receptor MerTK in the Development of Kidney Ischemia-Reperfusion Injury in RCS Rats

International audienceRenal ischaemia reperfusion (I/R) triggers a cascade of events including oxidative stress, apoptotic body and microparticle (MP) formation as well as an acute inflammatory process that may contribute to organ failure. Macrophages are recruited to phagocytose cell debris and MPs. The tyrosine kinase receptor MerTK is a major player in the phagocytosis process. Experimental models of renal I/R events are of major importance for identifying I/R key players and for elaborating novel therapeutical approaches. A major aim of our study was to investigate possible involvement of MerTK in renal I/R. We performed our study on both natural mutant rats for MerTK (referred to as RCS) and on wild type rats referred to as WT. I/R was established by of bilateral clamping of the renal pedicles for 30′ followed by three days of reperfusion. Plasma samples were analysed for creatinine, aspartate aminotransferase (ASAT), lactate dehydrogenase (LDH), kidney injury molecule -1 (KIM-1), and neutrophil gelatinase-associated lipocalin (NGAL) levels and for MPs. Kidney tissue damage and CD68-positive cell requirement were analysed by histochemistry. monocyte chemoattractant protein-1 (MCP-1), myeloperoxidase (MPO), inducible nitric oxide synthase (iNOS), and histone 3A (H3A) levels in kidney tissue lysates were analysed by western blotting. The phagocytic activity of blood-isolated monocytes collected from RCS or WT towards annexin-V positive bodies derived from cultured renal cell was assessed by fluorescence-activated single cell sorting (FACS) and confocal microscopy analyses. The renal I/R model for RCS rat described for the first time here paves the way for further investigations of MerTK-dependent events in renal tissue injury and repair mechanisms

Preparation of adipose tissue stromal vascular fraction by mechanical digestion for point of care application in veterinary medicine.

Preparation of adipose tissue stromal vascular fraction by mechanical digestion for point of care application in veterinary medicine. OBJECTIVES: Mesenchymal Stem Cells (MSCs) derived from adipose tissue have a growing role in the therapy of a variety of animals diseases, including wound healing, tendonitis, bone defects, osteoarticular pathologies and neurodegenerative diseases. As a matter of fact, fat tissue represents a complex source of cells as well as bioactive factors able to contribute to the healing of injured tissues. The term Stromal Vascular Fraction (SVF) has been introduced in recent years to describe the mixed cell population (endothelial cells, pericytes, smooth muscle cells, stromal progenitor cells) isolated from fat tissue. We evaluated the feasibility of a simple procedure for the preparation of a SVF derived from fat in the dog, to be used “point of care” ”, i.e. shortly after the collection of the biological sample and after a minimal manipulation. A key concept of the approach we propose, is that fat tissue is a source of MSCs but also of other cell types able, for example, to promote the formation of blood vessels and to modulate the different phases of tissue healing. MATERIALS AND METHODS: SVF was prepared by mechanical treatment of 2 grams of subcutaneous and visceral fat. A mechanical digestion was performed inside a 50 ml test tube using a set of 6 mm diameter sterile steel balls obtaining tissue fragments ranging from 1 mm3 to 4 mm3. The tissue fragments were cultured either on plastic surface or inside a three-dimensional scaffold prepared with Platelet Rich Plasma, thrombin and culture medium. Cell cultures were maintained until passage four for cells grown on plastic surface, or for 10-12 days for cells grown inside the three-dimensional matrix. RNA was collected to analyze the expression of markers typical for MSCs as well as endothelial cells and pericytes. RESULTS: The fragments released in the surrounding environment a high number of actively proliferating cells that populated the plastic surface or the three-dimensional gel in different culture conditions. Noteworthy, complex multicellular structures connecting different tissue fragments were often observed, thus suggesting the ability of the cells to respond to chemotactic signaling. The histological examination of the cultured fragments demonstrated that the tissue maintained its structural organization for at least 10-12 days. In addition, the cells released by tissue fragments adhered to the culture plate and were able to differentiate into adipocytes and osteoblasts. RT- PCR analysis demonstrated that cells expressing phenotypes of MSCs and endothelial cells are present in cell cultures. CONCLUSIONS: Although incomplete, the preliminary characterization of the cells obtained by the in-vitro culture of SVF, let us hypothesize that the cell population include MSCs maintaining their typical features. The safeguard of the tissue architecture and the long-lasting maintenance of its vitality guarantee a positive evaluation of the efficacy and efficiency of the proposed mechanical treatment

Biomolecular Recognition with Calixarene Derivatives by a MS-Based Chemoproteomic Approach

In questa presentazione orale è stata discussa la procedura proteomica per l'individuazione della proteina PDI come miglior partner del derivato calixarenico selezionato. Sono stati evidenziati i passaggi sperimentali come l'ancoraggio alla matrice, l'incubazione con il lisato cellulare di cellule HeLa e infine il lavaggio, elettroforesi e analisi proteomica delle bande selezionate, fino a individuare la PDI come migliore proteina più affine al calixarene recettore progettato