Construction of Synthetic Intact Human Parathyroid Hormone Gene and Testing the Transformation Efficiency and Expression of it in E. coli strains

 human parathyroid hormone gene (hPTH gene) is responsible for preproparathyroid hormone production (biologically inactive hormone) which further modified to form the biologically active hormone, a synthetic hPTH gene with 289bp which cod for active intact hPTH was constructed by Five long primers by splicing overlap extension PCR (SOE), the gene contain a de generated codon, and have two recognition site of restriction enzymes in both ends of the Gene, and a stop codon. The synthetic hPTH gene was transformed in two E. coli strains DH5? and BL21 (DE3) with transformation efficiency6.6* 106 and 3.4* 106 for both isolatesrespectively. And the transformation of gene was detected by extraction of constructed plasmid and amplification of cloning gene on it, the expression of the cloning gene in the BL21 (DE3) was detected by performing Real Time PCR which gave a Ct value of about (23.46). That makes the synthetic gene suitable for direct expression of human active protein inside the bacterial cells

Effects of Acetaminophen and Vitamin C Combination on Clinical Vital Signs and Behavioral Traits of Heat-Stressed Dairy Buffalo

Background: Heat stress (HS) presents significant challenges to water buffalo breeding, particularly in harsh environments like southern Iraq. HS adversely impacts physiological and behavioral aspects in dairy buffaloes, leading to economic losses in productivity and welfare. Interventions such as acetaminophen and vitamin C supplementation have been suggested to alleviate HS impacts due to their anti-stress and anti-inflammatory characteristics.Methods: This study was conducted in Al-Chibayish Marshes southern Iraq and aimed to evaluate the effects of acetaminophen and vitamin C combination (AVC) on heat-stressed dairy buffalo. Sixteen lactating buffaloes were divided into four treatments. One treatment received regular water (control), while other treatments were given water mixed with AVC at a dose of 500g/1000 liters for 3, 5, and 7 consecutive days per week, respectively, over 30 days. Environmental (temperature, humidity, and THI) and physiological data (body temperature, respiration rate, and pulse rate) were recorded daily, while feed and water consumption were recorded weekly.Results: Buffaloes experienced significant HS, with temperatures reaching up to 53.2°C and corresponding THI values ranging from 88.05 to 99.31. AVC administration for 5 consecutive days led to increased feed intake by 1.15 kg daily per treatment and decreased water consumption by 16.43 liters daily per treatment compared to the control. Additionally, rectal temperature, respiration rate, and heart rate showed progressive improvement during the second and third weeks, with the most notable improvement observed in the fourth week of the study. These improvements in vital signs and behavioral traits indicate the ability of AVC to improve thermoregulation and reduce heat stress impact.Conclusion: The investigation highlights the potential of AVC supplementation showing a promise in alleviating HS effects on dairy buffaloes by positively influencing physiological and behavioral parameters. Further research is needed to elucidate the underlying mechanisms and optimize supplementation strategies for enhancing buffalo welfare and productivity under heat stress.Keywords: Acetaminophen; Vitamin C; Physiological; Behavioral; Heat stress; Buffalo 

A novel nomadic people optimizer-based energy-efficient routing for WBAN

In response to user demand for wearable devices, several WBAN deployments now call for effective communication processes for remote data monitoring in real time. Using sensor networks, intelligent wearable devices have exchanged data that has benefited in the evaluation of possible security hazards. If smart wearables in sensor networks use an excessive amount of power during data transmission, both network lifetime and data transmission performance may suffer. Despite the network's effective data transmission, smart wearable patches include data that has been combined from several sources utilizing common aggregators. Data analysis requires careful network lifespan control throughout the aggregation phase. By using the Nomadic People Optimizer-based Energy-Efficient Routing (NPO-EER) approach, which effectively allows smart wearable patches by minimizing data aggregation time and eliminating routing loops, the network lifetime has been preserved in this research. The obtained findings showed that the NPO method had a great solution. Estimated Aggregation time, Energy consumption, Delay, and throughput have all been shown to be accurate indicators of the system's performance

Global economic burden of unmet surgical need for appendicitis

Background: There is a substantial gap in provision of adequate surgical care in many low-and middle-income countries. This study aimed to identify the economic burden of unmet surgical need for the common condition of appendicitis. Methods: Data on the incidence of appendicitis from 170 countries and two different approaches were used to estimate numbers of patients who do not receive surgery: as a fixed proportion of the total unmet surgical need per country (approach 1); and based on country income status (approach 2). Indirect costs with current levels of access and local quality, and those if quality were at the standards of high-income countries, were estimated. A human capital approach was applied, focusing on the economic burden resulting from premature death and absenteeism. Results: Excess mortality was 4185 per 100 000 cases of appendicitis using approach 1 and 3448 per 100 000 using approach 2. The economic burden of continuing current levels of access and local quality was US $92 492 million using approach 1 and$73 141 million using approach 2. The economic burden of not providing surgical care to the standards of high-income countries was $95 004 million using approach 1 and$75 666 million using approach 2. The largest share of these costs resulted from premature death (97.7 per cent) and lack of access (97.0 per cent) in contrast to lack of quality. Conclusion: For a comparatively non-complex emergency condition such as appendicitis, increasing access to care should be prioritized. Although improving quality of care should not be neglected, increasing provision of care at current standards could reduce societal costs substantially

Pooled analysis of WHO Surgical Safety Checklist use and mortality after emergency laparotomy

Background The World Health Organization (WHO) Surgical Safety Checklist has fostered safe practice for 10 years, yet its place in emergency surgery has not been assessed on a global scale. The aim of this study was to evaluate reported checklist use in emergency settings and examine the relationship with perioperative mortality in patients who had emergency laparotomy. Methods In two multinational cohort studies, adults undergoing emergency laparotomy were compared with those having elective gastrointestinal surgery. Relationships between reported checklist use and mortality were determined using multivariable logistic regression and bootstrapped simulation. Results Of 12 296 patients included from 76 countries, 4843 underwent emergency laparotomy. After adjusting for patient and disease factors, checklist use before emergency laparotomy was more common in countries with a high Human Development Index (HDI) (2455 of 2741, 89.6 per cent) compared with that in countries with a middle (753 of 1242, 60.6 per cent; odds ratio (OR) 0.17, 95 per cent c.i. 0.14 to 0.21, P <0001) or low (363 of 860, 422 per cent; OR 008, 007 to 010, P <0.001) HDI. Checklist use was less common in elective surgery than for emergency laparotomy in high-HDI countries (risk difference -94 (95 per cent c.i. -11.9 to -6.9) per cent; P <0001), but the relationship was reversed in low-HDI countries (+121 (+7.0 to +173) per cent; P <0001). In multivariable models, checklist use was associated with a lower 30-day perioperative mortality (OR 0.60, 0.50 to 073; P <0.001). The greatest absolute benefit was seen for emergency surgery in low- and middle-HDI countries. Conclusion Checklist use in emergency laparotomy was associated with a significantly lower perioperative mortality rate. Checklist use in low-HDI countries was half that in high-HDI countries.Peer reviewe

Global variation in anastomosis and end colostomy formation following left-sided colorectal resection

Background End colostomy rates following colorectal resection vary across institutions in high-income settings, being influenced by patient, disease, surgeon and system factors. This study aimed to assess global variation in end colostomy rates after left-sided colorectal resection. Methods This study comprised an analysis of GlobalSurg-1 and -2 international, prospective, observational cohort studies (2014, 2016), including consecutive adult patients undergoing elective or emergency left-sided colorectal resection within discrete 2-week windows. Countries were grouped into high-, middle- and low-income tertiles according to the United Nations Human Development Index (HDI). Factors associated with colostomy formation versus primary anastomosis were explored using a multilevel, multivariable logistic regression model. Results In total, 1635 patients from 242 hospitals in 57 countries undergoing left-sided colorectal resection were included: 113 (6·9 per cent) from low-HDI, 254 (15·5 per cent) from middle-HDI and 1268 (77·6 per cent) from high-HDI countries. There was a higher proportion of patients with perforated disease (57·5, 40·9 and 35·4 per cent; P < 0·001) and subsequent use of end colostomy (52·2, 24·8 and 18·9 per cent; P < 0·001) in low- compared with middle- and high-HDI settings. The association with colostomy use in low-HDI settings persisted (odds ratio (OR) 3·20, 95 per cent c.i. 1·35 to 7·57; P = 0·008) after risk adjustment for malignant disease (OR 2·34, 1·65 to 3·32; P < 0·001), emergency surgery (OR 4·08, 2·73 to 6·10; P < 0·001), time to operation at least 48 h (OR 1·99, 1·28 to 3·09; P = 0·002) and disease perforation (OR 4·00, 2·81 to 5·69; P < 0·001). Conclusion Global differences existed in the proportion of patients receiving end stomas after left-sided colorectal resection based on income, which went beyond case mix alone

Cover Image, Volume 138, Issue 25

This cover designed by Muhammad Irfan and colleagues shows how High Performance Polyethylene (HPPE) fibers are relatively tougher than carbon fibers and can be used to improve impact tolerance of carbon epoxy composite laminates. Composite laminates containing carbon fibers suffer from delamination upon impact. An interleave of a tough material like HPPE fibers inserted between these carbon fiber plies can reduce the damage due to impact. HPPE fibers do not undergo brittle fracture, rather ductile fracture is observed along with fibrillation. Nonwoven interleaves comprising HPPE fibers can be used to improve impact properties of carbon-epoxy composite laminates for various applications

Enhanced interlaminar shear and impact performance of woven carbon/epoxy composites interleaved with needle punched high performance polyethylene fiber nonwoven

In this study, high-performance polyethylene (HPPE) fiber-based needle punched nonwovens were interleaved in cross-plied woven carbon fabric/epoxy composite laminates to enhance their interlaminar and impact properties. The placement of needle punched nonwoven interleaves exhibited considerable enhancement in interlaminar shear strength (ILSS), impact damage tolerance, and compression after impact (CAI) strength of laminates as evidenced by higher interlaminar strength, less absorbed energy, higher elastic energy, reduced damage degree, reduced out-of-plane deformation, higher load-bearing capacity, and higher residual compressive strength as compared to control sample. In particular, the composite laminate with placement of interleaves in alternating sequence between carbon plies resulted in 205.76% increase in ILSS and 129, 103 and 85% increase in CAI at 10, 25, and 40 J impact energy, respectively. Moreover, damaged surface area and out-of-plane deformation reduced to 38.75% and 62.5%, respectively for the same specimen impacted at 40 J energy. These results suggest that the HPPE fiber-based needle punched nonwoven interleaving can be adopted as a simple and low-cost approach compared with other interleaving techniques, to enhance the resistance to delamination, impact performance, and damage tolerance of traditional structural laminates