Nutritive effect of cabbage (Brassica oleracea) on growth, obesity, lipidaemia and haematology in broiler and pullet chickens

Efficacy of cabbage (Brassica oleracea) for ameliorating the adverse metabolic syndrome side-effects of genetically improved growth rate in chickens was examined. Sixty-four (64) day-old birds (32 Marshall Broilers and 32 Harco Black Pullets) were randomly assigned to groups of eight genotype-matched birds in a 2-factor layout within a Completely Randomized Design (CRD). Within genotype, each group was randomly assigned one of four diets (basal/control diet containing 0% cabbage, and, basal diet supplemented with 3%, 6% or 12% cabbage) for 4 weeks. All birds were fed an un-supplemented Broiler finisher diet from week 4 - 8. Bodyweight and Body Mass Index (BMI) were determined weekly. Blood Packed Cell Volume (PCV), Haemoglobin (Hb), White Blood Cells (WBC), Red Blood Cells (RBC), neutrophils, lymphocytes, monocytes, basophils and eosinophils), Total serum Cholesterol (TC), High Density Lipoprotein (HDL) and Low Density Lipoprotein (LDL) were determined at age 4 weeks. Irrespective of diet or age, Broilers exhibited significantly greater (p<0.05) body weight, adiposity, and lipidaemia than Pullets, and no interactions between cabbage and genotype were observed for the same traits. Dietary cabbage at 3%, irrespective of genotype significantly (p<0.05) improved body weight beyond week 5 while no significant effect was observed on body fatness as measured by BMI. Cabbage supplementation suppressed broiler eosinophil levels, indicating effects on mediators of innate immune surveillance, but did not influence any other blood haematological  parameter, though Broilers exhibited higher (p<0.05) total WBC count and proportion (%) of WBC represented by neutrophils, basophils and eosinophils. In Contrast, Pullets exhibited higher RBC, PCV, and Hb, and lymphocyte and monocyte differential counts. Dietary Cabbage had a nutrigenetic effect on cholesterol: Reduced TC and LDL in Broilers in contrast to its effect in increasing the same lipids in Pullets. Cabbage however lowered HDL Cholesterol (p<0.05) in both genotypes, though the threshold of effect was higher (12%) in Pullets (3%). Thus Cabbage is effective in lowering heart disease risk through lowering of lipidaemia in Broilers, and improves bodyweight at market age (7-8 weeks) at 3% dietary supplementation level.KEYWORDS: Cabbage, Broiler, Pullet, Nutritive, Metabolic syndrome

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Pharmacological Exposure of Pregnant Mothers in Ilorin, Nigeria

Context: Use of drug during pregnancy is a universal event. However, in developing countries, drug use is poorly controlled as most of the drugs could be obtained without prescription. This constitutes potential hazard both for the mother and the fetus.Objective: To determine the pattern of drug use (including alcohol and smoking) prescription and non prescription of drug by pregnant mothers in Ilorin, Kwara State, Nigeria.Study Design: A cross sectional study of pregnant mothers attending antenatal clinic of the University of Ilorin Teaching Hospital, Ilorin.Result: Four hundred 400 pregnant women participated in the study, 335 adequately completed questionnaire for data analysis. Majority of the respondents 273 (81.5%) had taken one or more drugs, 62 (18.5%) had not used any. Average number of drugs used was 2.7. Routine haematinics (folic acid and Ferrous Sulphate) are the common drug taken by 241 (81.5%) of the respondents. Anti malarial drugs were taken by 47 (14%) while herbal drug was taken by 41 (12.2%). Five (1.5%) of the respondents admitted to taking alcohol and only one woman (0.3%) admitted to smoking cigarette. One (0.3%) each of the respondents was on anti depressant and anticonvulsant drugs. Occupation was significantly related to herbal use. Parity was also significantly related to alcohol consumption and the use of unprescribed drugs.Conclusion: Drug use is common during pregnancy. Haematinics were the commonest drug used. Herbs are used to some extent and antimalarials are underutilized. Alcohol is minimally used and smoking during pregnancy is virtually non- existent. There is need to caution our pregnant mothers on the use of herbs as there is no full understanding of their pharmacokinetics

Pregnancy Outcome in Cervical Incompetence: Comparison of Outcome Before and After Intervention

Context: Cervical incompetence is a major cause of recurrent mid-trimester pregnancy loss and preterm deliveries; it contributes significantly to fetal loss and neonatal morbidity and mortality. Despite its wide use, the effectiveness of cervical cerclage in its management remains unsettled.Objective: To evaluate the effectiveness of cervical cerclage by comparing the pregnancy outcome before and after its insertion in women with cervical incompetence.Study design: An observational study [retrospective] of 95 women  diagnosed with cervical incompetence that had cervical cerclage inserted from 1st January 2007 to31st December 2010. The pregnancy outcomebefore and after cervical cerclage were compared, the data was analyzed using SPSS version 18; p value <0.05 was considered significant.Main outcome measure: The gestational age at the end of pregnancy, the duration of prolongation of the pregnancy after cervical cerclage and the pregnancy outcome.Results: Of 103 cases of cervical incompetence managed, 95 satisfied the inclusion criteria. The prevalence of cervical incompetence was 8.4/1000 deliveries or 0.85%. There were 85 elective and 10 emergency cerclage with mean gestational age at end of pregnancy of 36.06±3.96 vs. 25.10±3.99 and mean duration of prolongation of pregnancy 20.98±4.71 vs. 4.00±3.37 weeks. After cervical cerclage insertion, there was reduction in miscarriages [P<0.0001] and preterm deliveries [P<0.0001] and increase in term deliveries [P=0.4100] and viable pregnancies [P=0.001]. The child take home rate was 89.4% following elective and 20% after emergency cervical cerclage.Conclusion: Cervical cerclage resulted in improved pregnancy outcome in women with previous midtrimester losses or preterm delivery.Keywords: Cervical cerclage; cervical incompetence; pregnancy outcome; intervention

Umbilical cord parameters in Ilorin: correlates and foetal outcome

Background: The anthropometric parameters of the umbilical cord have clinical significance. Current parameters of the cord, its correlates and related foetal outcome are lacking in our parturients.Objectives: To describe the anthropometric parameters and abnormalities of the umbilical cord; and determine their maternal correlates and foetal outcome.Design: A cross sectional analytical study.Setting: The Obstetric and Gynaecology Department of the University of Ilorin Teaching Hospital, between September 2012 and June 2013.Subjects: Healthy pregnant women with singleton pregnancies.Results: Four hundred and twenty- eight (428) singleton deliveries were studied. The respective mean values of the cord length and width were 526.87±115.5mm and 19.56±11.12mm. Short cord (< 40cm) occurred in 7.2% while long cord (> 69cm) was found in 9.3% of the parturient. The incidences of single umbilical artery, cord round the body and knots were 7%, 8.4% and 14.5% respectively. Nuchal cord was the most common (91.4%). Only gestational age had significant statistical relationship with cord length abnormalities (P = 0.0093). The cord length was an important correlate of cord helices, knots and vessels (P< 0.05).Parity had correlations with the number of vessels(R= 0.099, P=0.042). The cord coiling index was statistically related to the presence of congenital abnormalities (P=0.011). Other perinatal events were not related to umbilical cord parameters. Perinatal asphyxia was the most common indication for NICU admission (3.5%) but there was no significant statistical difference between NICU admission and cord parameters.Conclusion: The umbilical cord parameters in apparently healthy parturients in Ilorin were comparable with others elsewhere. The cord length and helix are important correlates of gestational age and congenital abnormalities. Parity may be related to abnormal umbilical vessels. Cord length, coils, coil index and umbilical vessels should be examined post-natally

Attitudes to female genital mutilation/cutting among male adolescents in Ilorin, Nigeria

Background. The central role of males in female reproductive health issues in patriarchal societies makes them an important group in the eradication of female genital mutilation/cutting (FGM/C).Objectives. To determine knowledge about and attitudes to FGM/C among male adolescents, and their preparedness to protect their future daughters from it.Methods. A cross-sectional survey among male adolescent students in Ilorin, Nigeria. Participants completed a self-administered questionnaire after consent had been obtained from them or their parents. Statistical analysis was with SPSS version 20.0 (IBM, USA). A p-value of <0.05 was taken as significant.Results. Of 1 536 male adolescents (mean age 15.09 (standard deviation 1.84) years, range 14 - 19), 1 184 (77.1%) were aware of FGM/C, 514 (33.5%) supported female circumcision, 362 (23.6%) would circumcise their future  daughters, 420 (27.3%) were of the opinion that FGM/C had benefits, mostly as a necessity for womanhood (109, 7.1%), and 627 (40.8%) perceived it as  wickedness against females; 546 (35.5%) were aware of efforts to eradicate FGM/C, and 42.2% recommended education as the most important intervention to achieve this.Conclusion. Education and involvement in advocacy may transform male adolescents into agents for eradication of FGM/C

Characterisation of the Cell Line HC-AFW1 Derived from a Pediatric Hepatocellular Carcinoma

Current treatment of paediatric hepatocellular carcinoma (HCC) is often inefficient due to advanced disease at diagnosis and resistance to common drugs. The aim of this study was to generate a cell line derived from a paediatric HCC in order to expand research in this field. We established the HC-AFW1 cell line from a liver neoplasm of a 4-year-old boy through culturing of primary tumor specimens. The cell line has been stable for over one year of culturing and has a doubling time of 40 h. The tumour cells have an epithelial histology and express HCC-associated proteins such as Alpha-fetoprotein (AFP), Glypican 3, E-cadherin, CD10, CD326, HepPar1 and Vimentin. Forty-nine amino acids in exon 3 of β-Catenin that involve the phosphorylation sites of GSK3 were absent and β-Catenin is detectable in the cell nuclei. Cytogenetic analysis revealed large anomalies in the chromosomal map. Several alterations of gene copy numbers were detected by genome-wide SNP array. Among the different drugs tested, cisplatin and irinotecan showed effective inhibition of tumour cell growth in a proliferation assay at concentrations below 5 µg/ml. Subcutaneous xenotransplantation of HC-AFW1 cells into NOD/SCID mice resulted in fast growing dedifferentiated tumours with high levels of serum AFP. Histological analyses of the primary tumour and xenografts included national and international expert pathological review. Consensus reading characterised the primary tumour and the HC-AFW1-derived tumours as HCC. HC-AFW1 is the first cell line derived from a paediatric HCC without a background of viral hepatitis or cirrhosis and represents a valuable tool for investigating the biology of and therapeutic strategies for childhood HCC