Identification of Candida species Isolated From Vulvovaginal Candidiasis Patients by Chromgen agar and PCR-RFLP Method

This study focuses on diagnosis of Candida species causing Vulvovaginal Candidiasis using phenotype and genotype analyzing methods, and frequencies of candida species also using Vulvovaginal Candidiasis patients. 130 samples (100 from patients and 30 from non infected women) were collected and cultured on biological media. Identifying the yeasts, initially some phenotypic experiments were carried out such as germ tube, from motion of pseudohyphae and clamydospores in CMA+TW80 medium, API20 candida and CHROMagar Candida. Genomic DNA of all species were extracted and analyzed with PCR and subsequent Polymerase Chain Reaction - Restriction Fragments Length Polymorphism (PCR-RFLP) methods. Frequency of C. albicans, C. krusei, C. tropicalis , C. parapsilosis and C. glabrata were 46.4%, 31%, 18%, 7.2%, and 1.8%, respectively.The ITS1-ITS4 region was amplified and the Restriction enzyme Msp1 digests this region and was used to identify of candida species .Electrophoretically ribosomal DNA of C. albicans, C. krusei, C. tropicalis and C. glabrata produced two bands whereas the C. parapsilosis gave one band

IL-17 in Protective Immunity to Vaginal Candidiasis

Vulvovaginal candidiasis (VVC) is caused by Candida albicans affects a significant number of women during their reproductive ages. Th17 cells play a major role in coordinating the host defense in oropharyngeal candidiasis. In this study we investigated the involvement of the Th17 response in an animal model of vulvovaginal candidiasis (VVC). The present study aimed to shed light on detect concentration of the IL-17 of infected animal and control . A direct Enzyme Linked Immunosorbent Assay (ELISA) was used to quantify IL-17 concentrations in 30 infected animal with VVC and 10 control group. Rats were intravaginally inoculated with C.albicans, and vaginal lavage fluids, serum were evaluated for proinflammatory cytokine IL-17 The data suggest that IL-17, produced by vaginal cells, particularly CD4 T cells, detected in the vaginal wash and serum during the infection, reaching a maximum 14 days after the challenge

Determination of fungal and parasitic infections caused vaginitis: molecular identification of Candida parapsilosis in Al-Nasiriyah city, Iraq

هدفت الدراسة الحالية لتحديد انتشار طفيلي Trichomonas vaginalis وفطريات Candida spp. وكذلك التعرف عن Candida parapsilosis وبعض جينات الضراوة. اجريت هذه الدراسة في مستشفى بنت الهدى للولادة والاطفال في محافظة ذي قار, جنوب العراق للفترة من بداية شهر كانون الثاني الى نهاية شهر كانون الاول 2020. تم جمع 250 عينة من المنطقة التناسلية الانثوية لنساء تتراوح اعمارهم من17-50 سنة. استخدم كل من التشخيص المجهري والتقليدي والجزيئي في فحص العينة. سجلت النتائج 12 (4.8%) عينة مصابة بطفيلي T. vaginalis, بينما 130 (52%) عينة اظهرت مصابة بـ Candida spp. والتي توزعت كالاتي: 75 (30%) C. albicans, 20 (8%) C. krusei, 14 (5.6%) C. parapsilosisas, 11 (4.4%) C. glabrata و 10 (4%) C. tropicalis. ظهر جين 18S rRNA  في كل عينات C. parapsilosisas التي اكدت مع الفحوصات الكيموحيوية ووسط CHROM. لوحظت الجينات cph1  و hwp1 في كل عزلات C. parapsilosis بنسبة (100%), بينما جينات sap1 و plb1 ظهرت مع نسب مختلفة (64.3%, 57.1%) على التوالي. اعتمادا على تحليل الشجرة التطورية, كان هناك تغاير جيني خفيف بين تتابعات العزلات المحلية مقارنة مع السلالات المسجله عالميا. اكدت الدراسة الحالية ان جين 18S rRNA يمتلك حساسية عالية في تشخيص C. parapsilosis. ان ظهور او غياب و/او وجود التغاير الجيني في بعض جينات الضراوة قد يسبب اعراض سريرية مختلفة.The current study aims to determine the prevalence of Trichomonas vaginalis and Candida spp., and also to identify Candida parapsilosis and some virulence genes. It was conducted in Bint Al-Hoda Hospital of Maternity and Children in Thi-Qar province, south of Iraq for the period from the beginning of January to the end of December 2020. Two hundred and fifty samples were collected from the female genital tract for women whose age ranged between 17-50 years. Microscopic, traditional and molecular tests were used in the sample examination. The results recorded 12 (4.8%) samples infected with T. vaginalis parasite, whereas 130 (52%) samples showed Candida yeast distributed as follows: 75 (30 %) C. albicans, 20 (8%) C. krusei, 14 (5.6%) C. parapsilosisas, 11 (4.4 %) C. glabrata and 10 (4%) C. tropicalis. A 18S rRNA gene of C. parapsilosisas appeared in all samples confirmed with biochemical tests and CHROM agar Candida. The cph1 and hwp1 genes were observed in all of C. parapsilosis isolates (100%), whereas sap1 and plb1 genes showed different proportions (64.3% and 57.1%, respectively). Depending on phylogenetic analysis, there was a slight genetic variation between local isolate sequences compared with global recorded strains.  The current study confirmed that 18S rRNA gene is highly precise to identify C. parapsilosis. The appearance or absence of the genetic variation of some virulence genes may cause different clinical manifestations.