Improving the Antibacterial Activity of Ceftazidime by Inulinase Purified from Staphylococcus Aureus

Inulinase is an enzyme catalyzing the hydrolysis of inulin, a plant stored polysaccharide, into fructoses and fructo- oligosaccharides that have a large spectrum of applications ranging from food industry to bioethanol production and pharmacology.Eight isolates of Staphylococcus aureus were isolated from agricultural rhizosphere soil samples with isolation percentage(32%)   and screened for higher inulinase production and found that Staphylococcus aureusS3 was the best producer. Inulinase was  partially purified with ammonium sulfate at 70% saturation and the specific activity reached to (7.01)U/mg protein. Inulinase led to enhancement ceftazidime activity against the bacteria and  gram positive bacteria more sensitive than negative bacteria to combination of inulinase and ceftazidime. These finding indicate that antistaphylococcal activity of ceftazidime antibiotic has increased in the presence of inulinase enzyme and the inulinase may be useful adjuvant agent for the treatment of S. aureus infections in combination with this antibiotic

SARS-CoV-2 vaccination modelling for safe surgery to save lives: data from an international prospective cohort study

Background: Preoperative SARS-CoV-2 vaccination could support safer elective surgery. Vaccine numbers are limited so this study aimed to inform their prioritization by modelling. Methods: The primary outcome was the number needed to vaccinate (NNV) to prevent one COVID-19-related death in 1 year. NNVs were based on postoperative SARS-CoV-2 rates and mortality in an international cohort study (surgical patients), and community SARS-CoV-2 incidence and case fatality data (general population). NNV estimates were stratified by age (18-49, 50-69, 70 or more years) and type of surgery. Best- and worst-case scenarios were used to describe uncertainty. Results: NNVs were more favourable in surgical patients than the general population. The most favourable NNVs were in patients aged 70 years or more needing cancer surgery (351; best case 196, worst case 816) or non-cancer surgery (733; best case 407, worst case 1664). Both exceeded the NNV in the general population (1840; best case 1196, worst case 3066). NNVs for surgical patients remained favourable at a range of SARS-CoV-2 incidence rates in sensitivity analysis modelling. Globally, prioritizing preoperative vaccination of patients needing elective surgery ahead of the general population could prevent an additional 58 687 (best case 115 007, worst case 20 177) COVID-19-related deaths in 1 year. Conclusion: As global roll out of SARS-CoV-2 vaccination proceeds, patients needing elective surgery should be prioritized ahead of the general population