Inhibition of monocyte complement receptor enhancement by low molecular weight material from human lung cancers

We have studied the effect of dialysates from lung cancer homogenates to alter both the expression of complement (C3b) receptors per se and also to inhibit leucoattractant-induced enhancement of complement rosettes on monocytes from healthy individuals. Enhancement and enhancement-inhibition by tumour extracts were compared with material derived from normal lung excised from distance from the tumour. There was no significant difference between tumour homogenate (TH) and normal lung homogenate (NLH) in terms of enhancement of complement rosettes per se. In contrast, TH produced a dose- and time-dependent inhibition of leucoattractant-induced enhancement of C3b rosettes which was significantly different from that obtained with NLH. This enhancement-inhibition was observed with four undifferentiated, four squamous and three adenocarcinomas of lung. The degree of enhancement-inhibition was not related to the type of tumour or varying accompanying histological features such as necrosis and the degree of infiltration with inflammatory cells. Following gel filtration on Sephadex G-50 each type of cancer gave a major peak of inhibitory activity which eluted with molecules having an apparent molecular size of approximately 3,000 daltons. A second larger peak (8,000-10,000 daltons) was also detected with extracts from the undifferentiated and adenocarcinomas. These results support previous findings, mainly from experimental animals, indicating that 'anti-macrophage/monocyte principles' are elaborated from certain tumour types

Quantifying temporal and spatial variations in sediment, nitrogen and phosphorus transport in stream inflows to a large eutrophic lake

High-frequency sampling of two major stream inflows to a large eutrophic lake (Lake Rotorua, New Zealand) was conducted to measure inputs of total suspended sediment (TSS), and fractions of nitrogen and phosphorus (P). A total of 17 rain events were sampled, including three during which both streams were simultaneously monitored to quantify how concentration–discharge (Q) relationships varied between catchments during similar hydrological conditions. Dissolved inorganic nitrogen (DIN) concentrations declined slightly during events, reflecting dilution of groundwater inputs by rainfall, whereas dissolved inorganic P (PO₄–P) concentrations were variable and unrelated to Q, suggesting dynamic sorptive behaviour. Event loads of total nitrogen (TN) were predominantly DIN, which is available for immediate uptake by primary producers, whereas total phosphorus (TP) loads predominantly comprised particulate P (less labile). Positive correlations between Q and concentrations of TP (and to a lesser extent TN) reflected increased particulate nutrient concentrations at high flows. Consequently, load estimates based on hourly Q during storm events and concentrations of routine monthly samples (mostly base flow) under-estimated TN and TP loads by an average of 19% and 40% respectively. Hysteresis with Q was commonly observed and inclusion of hydrological variables that reflect Q history in regression models improved predictions of TN and TP concentrations. Lorenz curves describing the proportions of cumulative load versus cumulative time quantified temporal inequality in loading. In the two study streams, 50% of estimated two-year loads of TN, TP and TSS were transported in 202–207, 76–126 and 1–8 days respectively. This study quantifies how hydrological and landscape factors can interact to influence pollutant flux at the catchment scale and highlights the importance of including storm transfers in lake loading estimates

Elastic Waves Along a Fracture Intersection

Fractures and fracture networks play a significant role in the subsurface hydraulic connectivity within the Earth. While a significant amount of research has been performed on the seismic response of single fractures and sets of fractures, few studies have examined the effect of fracture intersections on elastic wave propagation. Intersections play a key role in the connectivity of a fracture network that ultimately affects the hydraulic integrity of a rock mass. In this dissertation two new types of coupled waves are examined that propagate along intersections. 1) A coupled wedge wave that propagates along a surface fracture with particle motion highly localized to the intersection of a fracture with a free surface, and 2) fracture intersection waves that propagate along the intersection between two orthogonal fractures. Theoretical formulations were derived to determine the particle motion and velocity of intersection waves. Vibrational modes calculated from the theoretical formulation match those predicted by group theory based on the symmetry of the problem. For the coupled wedge wave, two vibrational modes exist that range in velocity between the wedge wave and Rayleigh wave velocity and exhibit either wagging or breathing motion depending on the Poisson\u27s ratio. For the intersection waves, the observed modes depend on the properties of the fractures forming the intersection. If both fractures have equal stiffness four modes exist, two with wagging and two with breathing motion. If the fractures have unequal stiffness, four modes also exist, but the motion depends on the Poisson\u27s ratio. The velocity of intersection waves depends on the coupling or stiffness of the intersection and frequency of the signal. In general, the different modes travel with speeds between the wedge wave and bulk shear wave velocity. Laboratory experiments were performed on isotropic and anisotropic samples to verify the existence of these waves. For both waves, the observed signals were determined to depend on the applied load, which affects the stiffness of the fractures. These results have significant implications for fracture network characterization using remote techniques in both the laboratory and the field. The coupling parameter used in this discussion, i.e., specific stiffness, is a potential parameter to link the hydraulic properties of the fracture intersections to their seismic response. These results are a first step towards remote characterization to determine the hydraulic connectivity of fracture networks