Production of single cell protein from stickwater of kilka fish meal factory using Lactobacillus acidophilus and Aspergillus niger

We investigated production of single cell protein (SCP) from stickwater of kilka fish meal factory as medium using Lactobacillus acidophilus and Aspergillus niger. Stickwater was used instead of the standard media of bacterium and fungus in a batch culture method. Amount of biomass, COD, RNA and protein in the bacterium and fungus in control and stickwater treatments were investigated. In maximum growth time, amino acids profile of the bacterium and fungus were measured and compared between treatments. Bacterial biomass production in the control and stickwater treatments were 3.16 and 5.12g/l, COD reduction was 33270 and 53330mg/l, the measured RNA were 15.27% and 15.04%, the amount of protein were 71.13% and 68.37%, respectively. The difference between bacterium and fungus biomass production was slight. We found that the amount of the fungus biomass in control and stickwater were 6.31 and 7.28g/l, COD reduction were 47800 and 55200mg/l, RNA was 9.36% and 9.09%, the amount of protein were 51.36% and 48.66%, respectively. In both bacterium and fungus, the maximum and minimum amount of amino acid of the control and stickwater was glutamic acid and methionin. The amount of methionin in bacterium was not different with fish meal and FAO reference and in fungus was a little lower than FAO reference. According to the results, application of pure stickwater was suitable for productionof Lactobacillus acidophilus and Aspergillus niger

Apparent lipid and fatty acid digestion, retention of lipid and growth performance in Caspian salmon (Salmo trutta caspius) fry fed dietary n-3 highly unsaturated fatty acids and vitamin E

A 10-week feeding experiment was conducted to investigate the effect of dietary n-3 highly unsaturated fatty acids (n-3 HUFA) and vitamin E interaction on growth, apparent digestibility, apparent retention and utilization of lipid in Caspian salmon fry. Six experimental diets combining three different dietary levels of n-3 HUFAs (L: low: 1+0.5, DHA+EPA, M: medium 2+1, DHA+EPA, H: high 4+2 DHA +EPA g/100g diet) with two different levels of vitamin E (L: low 300 and H: high 1000 mg/kg diet): LL, LH, ML, MH, HL and HH (HUFA/vitamin E) were investigated. Fry with initial mean (±SD) body weight of 600 ± 25 (mg) were randomly distributed in tanks and fed to apparent satiation. Increase in dietary HUFA and vitamin E markedly improved larval growth. The results showed that the survival of Caspian salmon fry was not affected by dietary omega-3 HUFA and vitamin E. C20:5n-3 and C22:6n-3 fatty acids content of polar and neutral lipids positively correlated with their concentration on diets. Apparent digestibility and apparent retention of total lipid was significantly affected by dietary HUFA. The results showed that n-3 highly unsaturated fatty acids and vitamin E had positive effects on growth performance, lipid metabolism and apparent digestibility of Caspian salmon fry

Transethnic analysis of the human leukocyte antigen region for ulcerative colitis reveals not only shared but also ethnicity-specific disease associations

Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gut. Genetic association studies have identified the highly variable human leukocyte antigen (HLA) region as the strongest susceptibility locus for IBD, and specifically DRB1*01:03 as a determining factor for ulcerative colitis (UC). However, for most of the association signal such a delineation could not be made due to tight structures of linkage disequilibrium within the HLA. The aim of this study was therefore to further characterize the HLA signal using a trans-ethnic approach. We performed a comprehensive fine mapping of single HLA alleles in UC in a cohort of 9,272 individuals with African American, East Asian, Puerto Rican, Indian and Iranian descent and 40,691 previously analyzed Caucasians, additionally analyzing whole HLA haplotypes. We computationally characterized the binding of associated HLA alleles to human self-peptides and analysed the physico-chemical properties of the HLA proteins and predicted self-peptidomes. Highlighting alleles of the HLA-DRB1*15 group and their correlated HLA-DQ-DR haplotypes, we identified consistent associations across different ethnicities but also identified population-specific signals. We observed that DRB1*01:03 is mostly present in individuals of Western European descent and hardly present in non-Caucasian individuals. We found peptides predicted to bind to risk HLA alleles to be rich in positively charged amino acids such. We conclude that the HLA plays an important role for UC susceptibility across different ethnicities. This research further implicates specific features of peptides that are predicted to bind risk and protective HLA proteins

Study on propagation and breeding of Hamoun fish (shizothoraxzarudnyi) to one gram weight in earthen pond

Hamoun fish, Schizothorax zarudnyi, is an indigenous species of the eastern waters of Iran, which is exclusively found in this region. However recently drought occurrence in the Chahnimeh reservoirs (a semi natural water body) making them vulnerable to extinction. As an appropriate action to address this problem and according to the 3 side contract between Italian Government – UNDP - Islamic republic of Iran Government and the Italian government financial support to reduce poverty in the Province of Sistan-Baluchestan through the reinforcement and dissemination of aquaculture activities the project was developed at the Hamoun Research Unit by the Chahbahar Fisheries Research Center to record the breeding normative of 1 g weight larvae for restocking and other researching purposes. 331 broodstocks of the indigenous species Shizothorax zarudnyi weighing 800-2450 g were collected from the Chahnimeh reservoirs in early autumn, 2006. From 5/3/2007 (the project is supported and communicated on 2010) , Ovulation was stimulated with three stimulators; pituitary extract (3-6 mg kg-1 body weight), GnRH-A (20-30 mg kg-1 body weight) and anti dopamine (10-15 mg kg-1 body weight) that was given in 2-3 doses to breeders. Of 169 injected breeders , some were injected On mid March of 2007 (12-13 ºC water temperature) responded to the injection 25% ,while the rest were injected On April of 2008 (14-16 ºC water temperature) responded to the injection 65%. In the present project of 167 breeders 82 were female and 87 male. Totally 30 female breeders released their eggs in different stages. 20 female breeders released their eggs completely, 3 breeders released half of their eggs and 7 released 1/3 of their eggs. The male breeders just injected in the final dose of hormontrapy and all were ready for releasing sperms however the ovulation in female breeders occurs between 353-428 h ºC and after the final dose of injection. Ripe eggs were stripped from the females and fertilization was done by the dry method. Fertilized eggs were transferred to veis incubators and troughs. Incubation period for eggs differs and larvae hatch out after about 910 days at an average water temperature of 12.5 °C. Maintained at 13-14 °C, complete absorption of yolk sac in Shizothorax zarudnyi larvae occurred after 5-8 days. Larvae were fed with a mixture of powdered milk and egg yolk in this stage followed by decapsulated Artemia cysts and nauplii of Artemia and then on formulated starter diets used for carps. Because the ponds were not ready, larvae were maintained in troughs for about ten days before they were transferred to two 1200 m2 earthen ponds where they reached a body weight of about 1 g. They were then handed over to the Iranian Fisheries department in the region. Larvae were fed with the starter feed SFCO in the earthen ponds. About 350 thousand larvae were stocked in two earthen ponds. Based on the results of present study and other studies we may conclude that artificial breeding in Schizothorax can be successfully achieved at 14-16 ºC in flow through systems using hormone therapy (combination of GnRha and anti dopamine) and larvae could be easily cultured in earthen ponds. However this species exhibits lower growth rates as compared to carps its high expenses could have an important role in economical feasible

Effect of metabolic genetic variants on long-term disease comorbidity in patients with type 2 diabetes

Underlying genetic determinants contribute to developing type 2 diabetes (T2D) future diseases. The present study aimed to identify which genetic variants are associated with the incident of the major T2D co-morbid disease. First, we conducted a discovery study by investigating the genetic associations of comorbid diseases within the framework of the Utrecht Cardiovascular Pharmacogenetic studies by turning information of > 25 years follow-up data of 1237 subjects whom were genotyped and included in the discovery study. We performed Cox proportional-hazards regression to examine associations between genetic variants and comorbid diseases including cardiovascular diseases (CVD), chronic eye disease, cancer, neurologic diseases and chronic kidney disease. Secondly, we replicated our findings in two independent cohorts consisting of 1041 subjects. Finally, we performed a meta-analysis by combining the discovery and two replication cohorts. We ascertained 390 (39.7%) incident cases of CVD, 182 (16.2%) of chronic eye disease, 155 (13.8%) of cancer, 31 (2.7%) of neurologic disease and 13 (1.1%) of chronic kidney disease during a median follow-up of 10.2 years. In the discovery study, we identified a total of 39 Single Nucleotide Polymorphisms (SNPs) associated with comorbid diseases. The replication study, confirmed that rs1870849 and rs8051326 may play a role in the incidence of chronic eye disease in T2D patients. Half of patients developed at least one comorbid disease, with CVD occurring most often and earliest followed by chronic eye disease. Further research is needed to confirm the associations of two associated SNPs with chronic eye disease in T2D

Survey and feasibility study for the introduction of native fish and non- native fish for cage culture in the southern part of Caspian Sea

The aim of this study is the feasibility of introducing suitable species of fish (native and exotic) for rearing in cages in the southern region of the Caspian Sea. This study from the perspective of economic efficiency and maintaining ecology were analyzed. The results showed that the southern Caspian Sea has the ability to aquaculture fish. However, due to the yearly temperature variations of water (from surface to a depth of 50 meters), the talent of aquaculture area is preferred for fish in cold water than warm water fish due to possibility rearing over the years. The survey showed that the current conditions and due to unfavorable changes in the ecology of the area in the last decade, the use of non-native fish culture in cages is not recommended. Many species of native fish are suitable for rearing in cages. Nevertheless, there is no infrastructure suitable for the production of all of them. Therefore, respectively species of Salmo caspius, Huso huso and Common carp of Caspian Sea for cultivation of in cages was suggested. In the current situation, this fish for their ability to grow faster than the need for selectivity. It is noteworthy that detailed environmental assessments and species risk assessments before the final introduction of any fish species (native and exotic) for rearing in cages in the Caspian Sea ecosystem is essential

Association analyses identify 38 susceptibility loci for inflammatory bowel disease and highlight shared genetic risk across populations

Cellular mechanisms in basic and clinical gastroenterology and hepatolog