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Mountain resilience: a tool for mudflow risk management in the Ile Alatau Mountains, Kazakhstan
The densely populated central part of the Ile Alatau mountains is one of the most mudflow-prone areas of Kazakhstan. Implementation of protection measures, early warning systems, and risk management plans is crucial to protect livelihoods and infrastructure from damage caused by mudflows. Increasing harm and damage from mudflows in recent decades—due to more frequent events, as well as increased economic development of the area—has made the establishment and implementation of a mudflow risk management system a priority. The effectiveness of such a system largely depends on the scientific validity of the management plan and hence is determined by the level of knowledge of the physical processes triggering such events. This knowledge is based on information that must be collected, analyzed, and systematized. However, such data are not easy to access; they are scattered over different archives and research institutes or simply missing. In recent years, scientific monographs, articles, and reports have been published that attempt to collect and systematize data on mudflow phenomena in general. These efforts provide a basis for further work but are often not readily available for use. This article presents the updateable, interactive, intelligent information system “Mudflow phenomena of the central part of the Ile Alatau” that links cartographic information with data on mudflow formation centers. This system concentrates and collates existing knowledge, making it accessible to stakeholders and decision-makers who can turn this knowledge into suitable applications for adaptive and sustainable risk management
Obtaining of Recombinant Camel Chymosin and Testing Its Milk-Clotting Activity on Cow’s, Goat’s, Ewes’, Camel’s and Mare’s Milk
In the cheese-making industry, commonly chymosin is used as the main milk-clotting enzyme. Bactrian camel (Camelus bactrianus) chymosin (BacChym) has a milk-clotting activity higher than that of calf chymosin for cow’s, goat’s, ewes’, mare’s and camel’s milk. A procedure for obtaining milk-clotting reagent based on recombinant camel chymosin is proposed here. Submerged fermentation by a recombinant yeast (Pichia pastoris GS115/pGAPZαA/ProchymCB) was implemented in a 50 L bioreactor, and the recombinant camel chymosin was prepared successfully. The activity of BacChym in yeast culture was 174.5 U/mL. The chymosin was concentrated 5.6-fold by cross-flow ultrafiltration and was purified by ion exchange chromatography. The activity of the purified BacChym was 4700 U/mL. By sublimation-drying with casein peptone, the BacChym powder was obtained with an activity of 36,000 U/g. By means of this chymosin, cheese was prepared from cow’s, goat’s, ewes’, camel’s and mare’s milk with a yield of 18%, 17.3%, 15.9%, 10.4% and 3%, respectively. Thus, the proposed procedure for obtaining a milk-clotting reagent based on BacChym via submerged fermentation by a recombinant yeast has some prospects for biotechnological applications. BacChym could be a prospective milk-clotting enzyme for different types of milk and their mixtures