PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC AND FLAVONOID CONTENTS OF ARISARUM VULGARE SEEDS

Interest in toxic plants is increasing because it is recognised that these plants contain bioactive compounds and then, have medicinal virtues. So, medicinal plants are of great importance to the health. Also, many papers have reported that the major pharmacological proprieties of medicinal plants are associated and attributed to their antioxidant activity, then; much attention is being paid to antioxidant compounds In this work, we targeted Arisarum vulgare plant growing in El-Kala National Park. This park is situated in the extreme North-East of Algeria (36°55'--36°90'N; 08°16'-08°43'E) is a specifi c site recognized for its fl oristic wealth, it presents a remarkable PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC AND FLAVONOID CONTENTS OF ARISARUM VULGARE SEEDS Hadjer Kadri, Salah Eddine Djilani, Abdelouaheb Djilani LSBO Department of Chemistry, Badji Mokhtar University B.P. 12, Annaba, Algeria ABSTRACT Background. Arisarum vulgare is screened and its total phenolic compounds and total fl avonoid contents were measured. In addition, the antioxidant capacity of the methanol-water (7:3) extract of this plant is evaluated by DPPH (2,2-diphenyl-1 picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic)) tests expressed by Vitamin C Equivalent Antioxidant Capacity (VCEAC). HPLC analyses are carried out to identify some polyphenols. The aim of this study is to identify, to quantify the phenolic compounds contained in the seeds of A. vulgare, and to evaluate their antioxidant capacity. Material and methods. Methanol-water (7:3) extract and three fraction obtained from silica gel column chromatography of A. vulgare were assayed by using RP-HPLC, spectrophotometric analyses, DPPH and ABTS tests. Results. Results obtained in the present study, revealed that total phenolic and fl avonoids of methanol-water extract of A. vulgare seeds were respectively 1.2 g GAE and 0.34 g QE per 100 g of plant extract dry weight. The total antioxidant capacity expressed as vitamin C equivalent antioxidant capacity (VCEAC) per 100 g of plant extract, obtained by ABTS and DPPH tests were respectively 1.3 g and 0.99 g VCE per 100 g dry weight. On the other hand RP-HPLC analyses reveal that the main phenolic compounds identified in the methanol-water (7:3) extract are gallic acid, caffeic acid and rutin. Conclusions. The results reveal that the methanol-water extract of A. vulgare seeds possesses strong antioxidative properties in vitro. Results confi rmed by high polyphenols and fl avonoids contents and corroborated by HPLC identifi cations

Chemical Composition and, Cellular Evaluation of the Antioxidant Activity of Desmodium adscendens Leaves

Desmodium adscendens plant is widely used as juice or tea in various parts of the world against a wide range of diseases. This study determines the quality and the quantity of polyphenols, flavonoids, anthocyanins, and tannins in D. adscendens leaves by UV-spectrophotometry and RP-HPLC methods. In addition, the antioxidant capacity of these phenolic compounds is evaluated by ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic)), DPPH (2,2-diphenyl-1 picrylhydrazyl), and Cellular tests. D. adscendens leaves are mainly composite of flavonoid compounds with 12.8 mg of catechin equivalent (CE)/g dw. The amounts of total polyphenol compounds are 11.1 mg of gallic acid equivalent (GAE)/g dw. The quantity of total anthocyanin and total tannin compounds is not considerable 0.0182 mg CgE/g dw and 0.39 mg CE/g dw, respectively. A direct correlation between phenolic compounds and antioxidant activity is observed (R2 = 0.96). The RP-HPLC analyses reveal that the main phenolic compound identified in the methanol-water extract is quercetrin dihydrat (2.11 mg/mL). According to the results, it is observed that D. adscendens leaves possess a considerable scavenging antioxidant and antiradical capacity, therefore these antioxidant properties might increase the therapeutic value of this medicinal plant

New extraction technique for alkaloids

A method of extraction of natural products has been developed. Compared with existing methods, the new technique is rapid, more efficient and consumes less solvent. Extraction of alkaloids from natural products such as Hyoscyamus muticus, Datura stramonium and Ruta graveolens consists of the use of a sonicated solution containing a surfactant as extracting agent. The alkaloids are precipitated by Mayer reagent, dissolved in an alkaline solution, and then extracted with chloroform. This article compares the results obtained with other methods showing clearly the advantages of the new method