5 research outputs found
Role of Matrix Metalloproteinase-9 in Neonatal Hypoxic-Ischemic Encephalopathy
BACKGROUND: Neonatal encephalopathy is a heterogeneous syndrome characterised by signs of central nervous system dysfunction in the newborn. Matrix metalloproteinase-9(MMP-9) increases the blood-brain barrier permeability, and their inhibitors can reduce its damage. MMP-9 has been implicated specifically in cerebral ischemia.
AIM: To measure serum MMP-9 in neonatal hypoxic-ischemic encephalopathy and evaluate its correlation to the severity of early prediction and treatment.
METHODS: its case-control study. The serum concentration of MMP-9 was determined by ELISA in 100 hypoxic neonates and 50 healthy neonates of matched age and sex who served as controls.
RESULTS: In our present study the serum MMP-9 level was significantly higher at p = 0.0001 in hypoxic-ischemic full-term newborns (176.7 ± 68.7 ng/ml)as compared to control newborn (69.4 ± 34.85 ng/ml)and it was significantly higher at p = 0.0075 in hypoxic-ischemic preterm newborn (171.2 ± 132.9 ng/ml) when compared to control newborn (72.54 ± 36.74 ng/ml),also MMP-9 was significantly higher at Sarnat stage III at p = 0.0001.
CONCLUSION: Serum MMP-9 level was significantly higher in hypoxic-ischemic newborns, and significantly increased with severity, so we suggest that serum MMP-9 level is important for predicting neurological sequel and severity in neonatal encephalopathy.
 
Isatin-benzoazine molecular hybrids as potential antiproliferative agents: synthesis and in vitro pharmacological profiling
Hatem A Abdel-Aziz,1 Wagdy M Eldehna,2 Adam B Keeton,3 Gary A Piazza,3 Adnan A Kadi,4 Mohamed W Attwa,4 Ali S Abdelhameed,4 Mohamed I Attia4,5 1Department of Applied Organic Chemistry, National Research Centre, Giza, 2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh, Egypt; 3Department of Oncologic Sciences and Pharmacology, Drug Discovery Research Center, Mitchell Cancer Institute, University of South Alabama, Mobile, AL, USA; 4Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; 5Medicinal and Pharmaceutical Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Giza, Egypt Abstract: In continuation of our endeavor with respect to the development of potent and effective isatin-based anticancer agents, we adopted the molecular hybridization approach to design and synthesize four different sets of isatin-quinazoline (6a–f and 7a–e)/phthalazine (8a–f)/quinoxaline (9a–f) hybrids. The antiproliferative activity of the target hybrids was assessed towards HT-29 (colon), ZR-75 (breast) and A-549 (lung) human cancer cell lines. Hybrids 8b–d emerged as the most active antiproliferative congener in this study. Compound 8c induced apoptosis via increasing caspase 3/7 activity by about 5-fold in the A-549 human cancer cell line. In addition, it exhibited an increase in the G1 phase and a decrease in the S and G2/M phases in the cell cycle effect assay. Furthermore, it displayed an inhibitory concentration 50% value of 9.5 µM against multidrug-resistant NCI-H69AR lung cancer cell line. The hybrid 8c was also subjected to in vitro metabolic investigations through its incubation with rat liver microsomes and analysis of the resulting metabolites with the aid of liquid chromatography-mass spectrometry. Keywords: isatins, hybridization approach, antiproliferative, apoptosi
Expression of resistance gene analogs in woodland strawberry (Fragaria vesca) during infection with Phytophthora cactorum
New Delhi metallo-β-lactamase-1 inhibitors for combating antibiotic drug resistance: recent developments
Recommended from our members
Beam-induced backgrounds measured in the ATLAS detector during local gas injection into the LHC beam vacuum
Abstract
Inelastic beam-gas collisions at the Large Hadron Collider
(LHC), within a few hundred metres of the ATLAS experiment, are
known to give the dominant contribution to beam backgrounds. These
are monitored by ATLAS with a dedicated Beam Conditions Monitor
(BCM) and with the rate of fake jets in the calorimeters. These two
methods are complementary since the BCM probes backgrounds just
around the beam pipe while fake jets are observed at radii of up to
several metres. In order to quantify the correlation between the
residual gas density in the LHC beam vacuum and the experimental
backgrounds recorded by ATLAS, several dedicated tests were
performed during LHC Run 2. Local pressure bumps, with a gas
density several orders of magnitude higher than during normal
operation, were introduced at different locations. The changes of
beam-related backgrounds, seen in ATLAS, are correlated with the
local pressure variation. In addition the rates of beam-gas events
are estimated from the pressure measurements and pressure bump
profiles obtained from calculations. Using these rates, the
efficiency of the ATLAS beam background monitors to detect beam-gas
events is derived as a function of distance from the interaction
point. These efficiencies and characteristic distributions of fake
jets from the beam backgrounds are found to be in good agreement
with results of beam-gas simulations performed with the
Fluka Monte Carlo programme.</jats:p