Enhanced Adaptive Neuro-Fuzzy Inference System Using Reptile Search Algorithm for Relating Swelling Potentiality Using Index Geotechnical Properties: A Case Study at El Sherouk City, Egypt

The swelling potentiality is a vital property of fine-grained soils strictly related to the index properties and chemical composition. The integration of machine learning techniques and geotechnical parameters provided a new integrative approach for predicting the free swelling index (FSI) and the swelling pressure (SP). In this paper, an adaptive neuro-fuzzy inference system (ANFIS) using named Reptile Search Algorithm (RSA) is presented to predict the swelling potentiality for fine-grained soils in the foundation bed at El Sherouk city, Egypt. The developed predictive model, named RSA-ANFIS, used as input measured 108 natural fine-grained soil samples of index geotechnical parameters and chemical composition as input data and the measured data of the free swelling index and the swelling pressure as output data. To justify the performance of the developed model, a comparative study was carried out, and the results show that the developed RSA-ANFIS has a high performance over the competitive methods in terms of coefficient of determination, root mean square error (RMSE), and mean absolute error (MAE). This new integrative approach is considered at the highly developed stage to predict and improve the analysis of multi-parameter soil behavior and could be applied in other objective variable datasets

The Implication of Petrographic Characteristics on the Mechanical Behavior of Middle Eocene Limestone, 15th May City, Egypt

The construction purposes of carbonate rocks are considered a major aspect of using these bedrocks based on their mechanical behavior. Accordingly, the physical and mechanical characterization of Middle Eocene Limestone bedrock in the new urban area at the 15th May City, Egypt was studied to assess the suitability of the carbonate rocks for construction. This study has been carried out to investigate the effect of petrographic characteristics on mechanical properties. To achieve this objective, the intact 30 rock core samples from 15 boreholes were selected at different depths. Based on study of the selected samples in thin sections, the limestone in the area was classified as lime-mudstone, wackestone, and grainstone. Additionally, the uniaxial compressive strength (UCS) and Schmidt Rebound Hammer (Rn) were determined to detect the mechanical properties of the limestone bedrock. The measured parameters (UCS and Rn) demonstrated a high direct relationship with mudstone and a poor direct relationship with dolomite and high negative correlation with wackestone and grainstone. Therefore, the Middle Eocene Limestone bedrock is more durable and has medium-strength, which made it suitable for constructions. Regression analysis was performed to find out some linear relationship between mechanical properties (UCS) with petrographic characteristics. The study reveals significant positive correlation between UCS and Rn with mudstone in accordance higher values of regression coefficient (R2 = 0.91 and R2 = 0.036), and an inverse relationship of Rn with dolomite % (R2 = 0.89 and R2 = 0.02), respectively. Consequently, the strong confidence on the mechanical parameters opens the way for engineers to predict the mechanical parameters that are required for engineering properties of limestone for the urban expansion

Simulation-Based Solutions Reducing Soil and Groundwater Contamination from Fertilizers in Arid and Semi-Arid Regions: Case Study the Eastern Nile Delta, Egypt

Intensive agriculture requires increasing application of fertilizers in order to sustain food production. Improper use of these substances in combination with increasing seawater intrusion results in long-term and nonpoint soil and groundwater contamination. In this work, a 3-D groundwater and solute transport numerical model was created to simulate the effect of excessive fertilizers application along the Bahr El Baqar drain system, in the eastern Nile Delta, Egypt. The geotechnical properties of the soils, hydrologic parameters, and unconfined compressive strength were determined at different sites and used as input parameters for the model. Model results showed that silty clay soils are able to contain the contaminations and preserve the groundwater quality. Nevertheless, sandy soils primarily located at the beginning of the Bahr El Baqar drain allow leakage of fertilizers to the groundwater. Thus, fertilizer application should be properly managed in the top sandy layers to protect the groundwater and soil, as increasing aquifer by excess irrigation water increased the groundwater contamination in confined layers due to the high value of cumulative salt for the current situation while the unconfined zone decreased groundwater and soil contamination. A mass transport 3-D multi-species (MT3D) model was set to identify the optimal measure to tackle soil and groundwater contamination along the Bahr El-Baqar drain system. A potential increase of the abstraction rates in the study area has a positive impact in reducing the transfer of fertilizer contamination to groundwater while it has a negative impact for soil contamination. The scenario analysis further indicated that the installation of a drainage network decreases the groundwater and soil contamination. Both solutions are potentially effective for protection against nonpoint contamination along the Bahr El Baqar drain system. However, a more sustainable management approach of fertilizer application is needed to adequately protect the receptors located further downstream in the Nile Delta

Mitigation of urban waterlogging from flash floods hazards in vulnerable watersheds

Study region: The Aswan region is a vast plateau in the South of Egypt located 150 m above mean sea level (AMSL). Within this region, the tourist city of Aswan is Egypt’s southernmost city, located on the east bank of the Nile River. Study focus: The occurrence of flash floods can severely impact low-lying and densely populated areas. Therefore, highly vulnerable areas require effective mitigation measures to guarantee public safety and preserve archeological sites of great importance. This study investigates the interaction between the soil, surface water, and groundwater in the Aswan region of Egypt. Based on the rainfall analysis and the watershed hydrology, six different scenarios were run using the MODFLOW and Watershed Modeling System (WMS) software, which simulated rainfall recurrence intervals of 2, 5, 10, 25, 50 and 100 years. New hydrological insights for the region: The model's results indicated that an increase in the recurrence interval produced a rise in the groundwater level (GWL) up to 8.82 m (AMSL). Therefore, constructing three dams was proposed as a solution at the three basins of Al- Haytah, Al-Kimab, and Umm-Buwayrat. The proposed solution allows the storage of large volumes of water upstream. It mitigates GWL's rise within and near Aswan City. The presented study can be applied to vulnerable watersheds in arid and semi-arid regions. It can help policymakers to integrate additional sustainable solutions into construction dams and their implementation in development plans

The immunomodulatory role of tumor Syndecan-1 (CD138) on ex vivo tumor microenvironmental CD4+ T cell polarization in inflammatory and non-inflammatory breast cancer patients.

Herein, we aimed to identify the immunomodulatory role of tumor Syndecan-1 (CD138) in the polarization of CD4+ T helper (Th) subsets isolated from the tumor microenvironment of inflammatory breast cancer (IBC) and non-IBC patients. Lymphocytes and mononuclear cells isolated from the axillary tributaries of non-IBC and IBC patients during modified radical mastectomy were either stimulated with the secretome as indirect co-culture or directly co-cultured with control and Syndecan-1-silenced SUM-149 IBC cells. In addition, peripheral blood mononuclear cells (PBMCs) of normal subjects were used for the direct co-culture. Employing flow cytometry, we analyzed the expression of the intracellular IFN-γ, IL-4, IL-17, and Foxp3 markers as readout for basal and co-cultured Th1, Th2, Th17, and Treg CD4+ subsets, respectively. Our data revealed that IBC displayed a lower basal frequency of Th1 and Th2 subsets than non-IBC. Syndecan-1-silenced SUM-149 cells significantly upregulated only Treg subset polarization of normal subjects relative to controls. However, Syndecan-1 silencing significantly enhanced the polarization of Th17 and Treg subsets of non-IBC under both direct and indirect conditions and induced only Th1 subset polarization under indirect conditions compared to control. Interestingly, qPCR revealed that there was a negative correlation between Syndecan-1 and each of IL-4, IL-17, and Foxp3 mRNA expression in carcinoma tissues of IBC and that the correlation was reversed in non-IBC. Mechanistically, Syndecan-1 knockdown in SUM-149 cells promoted Th17 cell expansion via upregulation of IL-23 and the Notch ligand DLL4. Overall, this study indicates a low frequency of the circulating antitumor Th1 subset in IBC and suggests that tumor Syndecan-1 silencing enhances ex vivo polarization of CD4+ Th17 and Treg cells of non-IBC, whereby Th17 polarization is possibly mediated via upregulation of IL-23 and DLL4. These findings suggest the immunoregulatory role of tumor Syndecan-1 expression in Th cell polarization that may have therapeutic implications for breast cancer

Characterization of inflammatory breast cancer: a vibrational microspectroscopy and imaging approach at the cellular and tissue level

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Inflammatory Breast Carcinoma: Elevated microRNA miR-181b-5p and Reduced miR-200b-3p, miR-200c-3p, and miR-203a-3p Expression as Potential Biomarkers with Diagnostic Value

Inflammatory breast cancer (IBC) is a rare yet aggressive breast cancer variant, associated with a poor prognosis. The major challenge for IBC is misdiagnosis due to the lack of molecular biomarkers. We profiled dysregulated expression of microRNAs (miRNAs) in primary samples of IBC and non-IBC tumors using human breast cancer miRNA PCR array. We discovered that 28 miRNAs were dysregulated (10 were upregulated, while 18 were underexpressed) in IBC vs. non-IBC tumors. We identified 128 hub genes, which are putative targets of the differentially expressed miRNAs and modulate important cancer biological processes. Furthermore, our qPCR analysis independently verified a significantly upregulated expression of miR-181b-5p, whereas a significant downregulation of miR-200b-3p, miR-200c-3p, and miR-203a-3p was detected in IBC tumors. Receiver operating characteristic (ROC) curves implied that the four miRNAs individually had a diagnostic accuracy in discriminating patients with IBC from non-IBC and that miR-203a-3p had the highest diagnostic value with an AUC of 0.821. Interestingly, a combination of miR-181b-5p, miR-200b-3p, and miR-200c-3p robustly improved the diagnostic accuracy, with an area under the curve (AUC) of 0.897. Intriguingly, qPCR revealed that the expression of zinc finger E box-binding homeobox 2 (ZEB2) mRNA, the putative target of miR-200b-3p, miR-200c-3p, and miR-203a-3p, was upregulated in IBC tumors. Overall, this study identified a set of miRNAs serving as potential biomarkers with diagnostic relevance for IBC