5\u27-nucleotidase, oxidative stress and antioxidant status in alcohol consumers and cirrhotic patients

Uvod: Cilj istraživanja bio je izmjeriti aktivnost enzima 5\u27-nukleotidaza kod bolesnika s cirozom jetre i osoba koje uzimaju alkohol. U istraživanju se ispitivao i oksidacijski stres, antioksidansi te njihova povezanost s 5\u27-nukleotidazom. Materijali i metode: Istraživanje je provedeno u tri skupine po 25 ispitanika jednake dobi i spola: I. skupina (kontrolni ispitanici), II. skupina (osobe koje uzimaju alkohol, tj. konzumenti alkohola) i III. skupina (bolesnici s cirozom jetre). Uzorci krvi prikupljeni od ispitanika centrifugirani su kako bi se odvojila plazma za analizu 5\u27-nukleotidaze. Odvojene stanice su tri puta isprane 0,9-postotnom hladnom fiziološkom otopinom i upotrebljene za analizu glutationa, malondialdehida i superoksid-dismutaze. Rezultati: Aktivnost 5\u27-nukleotidaze u serumu bila je statistički značajno povišena kod skupine bolesnika s cirozom i skupine konzumenata alkohola. Koncentracije malondialdehida bile su također statistički značajno povišene kod bolesnika s cirozom jetre i konzumenata alkohola. Koncentracije glutationa i superoksid-dismutaze bile su statistički značajno snižene u obje skupine. Zaključak: Iz ovih rezultata može se zaključiti da je aktivnost 5\u27-nukleotidaze u serumu dosljedno viša kod bolesnika s cirozom jetre i osoba koje uzimaju alkohol. Zapažena razlika mogla bi ukazivati na opseg oštećenja jetre, oštećenja hepatobilijarnog sustava i opstrukcije jetre.Background: The present study was undertaken to determine the 5\u27-nucleotidase enzyme activity in liver cirrhotic patients and alcohol consumers. Oxidative stress, antioxidants and their association with 5\u27-nucleotidase were also investigated. Methods: The study included three groups of 25 age and sex matched subjects: group I (control), group II (alcohol consumers) and group III (cirrhotic patients). Blood samples were collected and centrifuged for separation of plasma for analysis of 5\u27-nucleotidase. Separated cells were washed thrice with 0.9% w/v cold normal saline and used for the analysis of glutathione, malondialdehyde and superoxide dismutase. Results: The activity of serum 5\u27-nucleotidase was significantly increased in both cirrhotic patients and alcohol consumers. The levels of malondialdehyde were also significantly increased in both cirrhotic patients and alcohol consumers. The levels of glutathione and superoxide dismutase were significantly decreased in both cirrhotic patients and alcohol consumers. Conclusions: Study results indicated the activity of serum 5\u27-nucleotidase to be consistently higher in cirrhotic patients and alcohol consumers. The difference recorded might be pointing to the extent of liver damage, hepatobiliary damage, and biliary stasis

hypercholesterolemic patients

Abstract: This study was an attempt to establish the extent of increased oxidative stress in hypercholesterolemic patients and to evaluate the effect of atorvastatin on the oxidative stress and antioxidant status. Blood samples of 15 subjects (age and sex matched) from three groups: group I (healthy subjects), group II (hypercholesterolemic patients with atorvastatin treatment) and group III (hypercholesterolemic patients without any hypolipidemic drug) were taken and centrifuged to separate the plasma, which was used for the determination of vitamin E. The separated cells were washed thrice with 0.90 % w/v cold normal saline and used for the assay of the percentage hemolysis of the RBCs, and the determination of malondialdehyde, superoxide dismutase and hemoglobin. The levels of oxidative stress were higher in the hypercholesterolemic in comparison to the control and atorvastatin group. The levels of antioxidants were higher in the atorvastatin group than in the hypercholesterolemic one but were lower than the controls. From these findings, it was concluded that there is an increase in oxidative stress in hypercholesterolemia but it decreased significantly after 2 months of atorvastatin therapy and antioxidant status also improves in patients taking atorvastatin