Q Methodology: A Method for Understanding Complex Viewpoints in Communities Served by Extension

This article introduces Q methodology, an idea-sorting activity that can help Extension improve outreach and education on new and contentious issues. Q methodology is a helpful tool when Extension professionals are confronted with controversial or complex resource management challenges. Through the analysis of a simple card-sorting exercise, researchers can determine quantitatively and qualitatively how different issues combine to result in (a) an individual\u27s viewpoint on an issue and (b) groupings of different viewpoints within a community. We describe the basic approach to implementing Q methodology and suggest circumstances in which it can help facilitate Extension outreach and education

LSST Science Book, Version 2.0

A survey that can cover the sky in optical bands over wide fields to faint magnitudes with a fast cadence will enable many of the exciting science opportunities of the next decade. The Large Synoptic Survey Telescope (LSST) will have an effective aperture of 6.7 meters and an imaging camera with field of view of 9.6 deg^2, and will be devoted to a ten-year imaging survey over 20,000 deg^2 south of +15 deg. Each pointing will be imaged 2000 times with fifteen second exposures in six broad bands from 0.35 to 1.1 microns, to a total point-source depth of r~27.5. The LSST Science Book describes the basic parameters of the LSST hardware, software, and observing plans. The book discusses educational and outreach opportunities, then goes on to describe a broad range of science that LSST will revolutionize: mapping the inner and outer Solar System, stellar populations in the Milky Way and nearby galaxies, the structure of the Milky Way disk and halo and other objects in the Local Volume, transient and variable objects both at low and high redshift, and the properties of normal and active galaxies at low and high redshift. It then turns to far-field cosmological topics, exploring properties of supernovae to z~1, strong and weak lensing, the large-scale distribution of galaxies and baryon oscillations, and how these different probes may be combined to constrain cosmological models and the physics of dark energy.Comment: 596 pages. Also available at full resolution at http://www.lsst.org/lsst/sciboo

Global Analysis of the Evolution and Mechanism of Echinocandin Resistance in Candida glabrata

The evolution of drug resistance has a profound impact on human health. Candida glabrata is a leading human fungal pathogen that can rapidly evolve resistance to echinocandins, which target cell wall biosynthesis and are front-line therapeutics for Candida infections. Here, we provide the first global analysis of mutations accompanying the evolution of fungal drug resistance in a human host utilizing a series of C. glabrata isolates that evolved echinocandin resistance in a patient treated with the echinocandin caspofungin for recurring bloodstream candidemia. Whole genome sequencing identified a mutation in the drug target, FKS2, accompanying a major resistance increase, and 8 additional non-synonymous mutations. The FKS2-T1987C mutation was sufficient for echinocandin resistance, and associated with a fitness cost that was mitigated with further evolution, observed in vitro and in a murine model of systemic candidemia. A CDC6-A511G(K171E) mutation acquired before FKS2-T1987C(S663P), conferred a small resistance increase. Elevated dosage of CDC55, which acquired a C463T(P155S) mutation after FKS2-T1987C(S663P), ameliorated fitness. To discover strategies to abrogate echinocandin resistance, we focused on the molecular chaperone Hsp90 and downstream effector calcineurin. Genetic or pharmacological compromise of Hsp90 or calcineurin function reduced basal tolerance and resistance. Hsp90 and calcineurin were required for caspofungin-dependent FKS2 induction, providing a mechanism governing echinocandin resistance. A mitochondrial respiration-defective petite mutant in the series revealed that the petite phenotype does not confer echinocandin resistance, but renders strains refractory to synergy between echinocandins and Hsp90 or calcineurin inhibitors. The kidneys of mice infected with the petite mutant were sterile, while those infected with the HSP90-repressible strain had reduced fungal burden. We provide the first global view of mutations accompanying the evolution of fungal drug resistance in a human host, implicate the premier compensatory mutation mitigating the cost of echinocandin resistance, and suggest a new mechanism of echinocandin resistance with broad therapeutic potential

Using Watershed Assessments to Inform Planning for Rural Watersheds

9 pp.The Using Watershed Assessments to Inform Planning for Rural Watersheds publication provides a process for developing a baseline watershed assessment. In this guide we provide recommendations for engaging with stakeholders to assess natural resource conditions, as well as basic information to collect to create a baseline assessment. Watershed planning is not a simple, quick process. This guide addresses just the first steps of building a watershed assessment– understanding the current conditions and issues facing your watershed. Beyond the watershed assessment phase is the hard work of utilizing the information from the assessment, along with the results of additional stakeholder feedback, to develop an actual watershed plan. This guide provides an outline of how to complete the watershed assessment portion of your watershed planning effort, but does not provide a detailed step-by-step process. Rather, this document is intended as a resource to help guide you in your efforts by providing suggestions based on real-world watershed planning experience

The Effects of Federal Policies on Rangeland Ecosystem Services in the Southwestern United States

On the Ground • Rangelands provide a wide array of ecosystem services – the direct benefits people receive from nature. There is increasing interest by policymakers and conservationists in managing for these ecosystem services. • Because of complex land tenure arrangements inthe Intermountain West, it is important to understand the impacts of federal resource management laws on ecosystem services flowing from public and private lands. • All major federal land management laws are supportive of managing for ecosystem services. We review the implications of FLPMA, NFMA, NEPA, ESA, and CWA on ecosystem services on public and private lands.The Rangelands archives are made available by the Society for Range Management and the University of Arizona Libraries. Contact [email protected] for further information.Migrated from OJS platform March 202

Jaguar Critical Habitat Designation Causes Concern for Southwestern Ranchers

On the Ground • The designation of jaguar critical habitat in April 2014 in southern Arizona and southwestern New Mexico created concern for livestock ranchers in the region. • We interviewed ranchers to understand their concerns with the jaguar critical habitat designation and their attitudes toward jaguars, wildlife conservation, and resource management in general. • Ranchers we interviewed were concerned about direct impacts of designated critical habitat on ranching, as well as possible alternative agendas of critical habitat advocates and issues specific to the borderlands region. • The ranchers were less concerned about the presence of jaguars but were more concerned about possible limiting effects of the Endangered Species Act (ESA), distrust of government entities, and litigious environmental groups. • To maximize effectiveness, government agencies should work to foster trust in the ranching community, be cognizant of sensitive issues specific to the region that may challenge endangered species conservation goals, recognize the opportunity to work with ranchers for endangered species management, and provide outreach about implications of the ESA.The Rangelands archives are made available by the Society for Range Management and the University of Arizona Libraries. Contact [email protected] for further information.Migrated from OJS platform March 202

Foundations of Virtual Fencing: Training and Animal Welfare

In Arizona and other western states, ranchers and land managers rely on thousands of miles of permanent wire fencing to manage livestock on extensive rangelands (Hayter 1939; Netz 2004). This type of fencing has improved rangeland conditions in many places by aiding in the application of grazing systems (Holecheck et al. 2011). However, wire fencing can fragment landscape connectivity, pose a risk to wildlife, and is a major financial investment. Moreover, it offers limited flexibility in adjusting pasture size, actively manipulating grazing distribution, or avoiding high-use areas or sensitive habitats within a pasture (Jakes et al. 2018)

Correction: Igf Signaling is Required for Cardiomyocyte Proliferation during Zebrafish Heart Development and Regeneration.

Unlike its mammalian counterpart, the adult zebrafish heart is able to fully regenerate after severe injury. One of the most important events during the regeneration process is cardiomyocyte proliferation, which results in the replacement of lost myocardium. Growth factors that induce cardiomyocyte proliferation during zebrafish heart regeneration remain to be identified. Signaling pathways important for heart development might be reutilized during heart regeneration. IGF2 was recently shown to be important for cardiomyocyte proliferation and heart growth during mid-gestation heart development in mice, although its role in heart regeneration is unknown. We found that expression of igf2b was upregulated during zebrafish heart regeneration. Following resection of the ventricle apex, igf2b expression was detected in the wound, endocardium and epicardium at a time that coincides with cardiomyocyte proliferation. Transgenic zebrafish embryos expressing a dominant negative form of Igf1 receptor (dn-Igf1r) had fewer cardiomyocytes and impaired heart development, as did embryos treated with an Igf1r inhibitor. Moreover, inhibition of Igf1r signaling blocked cardiomyocyte proliferation during heart development and regeneration. We found that Igf signaling is required for a subpopulation of cardiomyocytes marked by gata4:EGFP to contribute to the regenerating area. Our findings suggest that Igf signaling is important for heart development and myocardial regeneration in zebrafish