Considerations Regarding the Antimicrobial Effect of Procid and Procid Forte Biocide Products

Procid and Procid forte products are containing two biocide substances (glutaraldehyd and didecyl-dimethyl ammonium chloride) with inhibitory action on Gram negative and Gram positive bacteria, viruses, fungi and unicellular algae. The products are recommended in the veterinary practice for microbial disinfection of various surfaces. The antimicrobial effect was tested for the following microorganism types: Gram negative bacteria: Salmonella sp., Escherichia coli, Pseudomonas pyocianea, Bordetella bronchiseptica, Neisseria polysacharea; Gram positive bacteria: Staphylococcus aureus, Bacillus anthracis (tulpina vaccinală 1190 R), Bacillus laterosporus, Brevibacterium linens, Listeria monocytogenes, Erisipelothrix rhusiopathiae; microscopic fungi: Penicillium sp., Aspergillus sp., Candida albicans; unicellular algae: Prototheca zopfii. The following reference strains of the ATTC collection were also tested: Staphylococcus aureus 6358P, Escherichia coli 10536, Pseudomonas pyocyanea 27853, Prototheca wickerhamii 16529. From both products dilutions of 1/100 and 1/200 (10 ml) were introduced in tubes and the tested culture was added (0,2 ml). Passages on culture mediums were performed after 30, 60 minutes and 24 hours, in order to test the mycrobicidal effect. All the tested strains were inhibiting by 1/100 dilution to the three contact times. At 1/200 dilution resistance was observed for Salmonella (at 30 minutes) and Pseudomonas (at 30 and 60 minutes)