COMPARISON OF FREQUENCY ANILINE DEGRADING BACTERIA IN THE SOIL AROUND SHIRAZ REFINERY IN SUMMER AND AUTUMN

ABSTRACT Aniline is one of the serious hazardous materials for life due to toxicity and carcinogenic effects. It is widely distributed in the soil and surrounding environment. Microbial degradation is one of the main mechanisms to remove it from environment. The aim of this research is to detect the indigenous aniline resistance bacteria from soil around Shiraz Refinery (Iran) and compare these for the term of two seasons in order to select the most powerful aniline resistant bacteria for bioremediation and clean a polluted soil. Sampling from the soil around Shiraz Refinery was done during summer and autumn and cultured in a medium containing 0.1 g/l aniline. Isolated bacteria were identified using the standard methods, these are known to be: Delftia acidovorans, Enterobacter ludwigii, Raoultella planticola, Alcaligenes faecalis, Serratia marcescens, Microbacterium barkeri, Pseudomonas acidovorans, Erwinia sp., Rhodococcus sp. Findings showed all of these bacteria were able to degrade aniline from the soil around Shiraz refinery and Delftia acidovorans and Rhodococcus sp. which was found during both time periods, had the highest ability to eliminate aniline