22 research outputs found
Germline IGHV3-53-encoded RBD-targeting neutralizing antibodies are commonly present in the antibody repertoires of COVID-19 patients
Plasma cell immunoglobulin heavy chain repertoire dynamics before and after tetanus booster vaccination
Structural basis for potent neutralization of SARS-CoV-2 and role of antibody affinity maturation
Leukocyte elastase and free collagenase activity in synovial effusions: Relation to numbers of polymorphonuclear leukocytes
Structural basis for broad neutralization of ebolaviruses by an antibody targeting the glycoprotein fusion loop
Protocadherin-1 is essential for cell entry by New World hantaviruses
International audienceThe zoonotic transmission of hantaviruses from their rodent hosts to humans in North and South America is associated with a severe and frequently fatal respiratory disease, hantavirus pulmonary syndrome (HPS)1,2. No specific antiviral treatments for HPS are available, and no molecular determinants of in vivo susceptibility to hantavirus infection and HPS are known. Here we identify the human asthma-associated gene protocadherin-1 (PCDH1)3-6 as an essential determinant of entry and infection in pulmonary endothelial cells by two hantaviruses that cause HPS, Andes virus (ANDV) and Sin Nombre virus (SNV). In vitro, we show that the surface glycoproteins of ANDV and SNV directly recognize the outermost extracellular repeat domain of PCDH1-a member of the cadherin superfamily7,8-to exploit PCDH1 for entry. In vivo, genetic ablation of PCDH1 renders Syrian golden hamsters highly resistant to a usually lethal ANDV challenge. Targeting PCDH1 could provide strategies to reduce infection and disease caused by New World hantaviruses