2 research outputs found
Priprava i ex vivo evaluacija TEC kao promotora apsorpcije tvari u kolonu
In previous studies, it was established that chitosan and its quaternized derivatives are potent enhancers of hydrophilic compounds absorption across intestinal epithelia. The aim of this study was to evaluate the application of a new quaternized chitosan, triethyl chitosan (TEC), in pharmaceutical approaches. TEC was synthesized by a one step process via a 22 factorial design to optimize the preparation conditions. In ex vivo experiments, everted rat colon sac was used to determine the effect of TEC on the penetration of hydrophilic compounds of different molecular masses (e.g., sodium fluorescein and brilliant blue) through colonic epithelia in comparison with chitosan at pH 7.4. These studies indicated a significant increase in absorption of sodium fluorescein and brilliant blue in the presence of TEC compared to chitosan. TEC bearing positive charge is able to interact with the tight junctions of colon epithelia and hence increase the permeation of sodium fluorescein and brilliant blue through the tight junctions. This investigation has shown that triethyl chitosan could be used as a penetration enhancer for poorly absorbable compounds in the colon drug delivery system.U ranijim istraživanjima utrÄeno je da su kitozan i njegovi kvartenizirani derivati snažni promotori apsorpcije hidrofilnih spojeva kroz intestinalnu sluznicu. Cilj rada bio je evaluirati novi kvartenizirani kitozan, trietil kitozan (TEC ).TEC je sintetiziran u jednom stupnju. U ex vivo eksperimentima na kolonu Å”takora praÄen je uÄinak tog polimera na penetraciju hidrofilnih spojeva razliÄitih molekulskih masa (fluorescein natrija i briljant plavila). Rezultati su usporeÄivani s uÄinkom kitozana na pH 7,4. PrimjeÄeno je da TEC znaÄajno poveÄava apsorpciju ispitivanih tvari u odnosu na nemodificirani kitozan. TEC svojim pozitivnim nabojem dolazi u interakciju s epitelom kolona i tako poveÄava njegovu permeabilnost. Ispitivanja ukazuju da se trietil kitozan može upotrijebiti kao promotor penetracije za spojeve koji se slabo apsorbiraju u kolonu