Selectivity in regeneration of the oculomotor nerve in the cichlid fish, Astronotus ocellatus

It has long been considered a general rule for nerve regeneration that the reinnervation of skeletal muscle is nonselective. Regenerating nerve fibers are supposed to reconnect with one skeletal muscle as readily as another according to studies covering a wide range of vertebrates (Weiss, 1937; Weiss & Taylor, 1944; Weiss & Hoag, 1946; Bernstein & Guth, 1961; Guth, 1961, 1962, 1963). Similarly, in embryogenesis proper functional connexions between nerve centers and particular muscles are supposedly attained, not by selective nerve outgrowth but rather through a process of ‘myotypic modulation’ (Weiss, 1955) that presupposes nonselective peripheral innervation. Doubt about the general validity of this rule and the concepts behind it has come from a series of studies on regeneration of the oculomotor nerve in teleosts, urodeles, and anurans and of spinal fin nerves in teleosts (Sperry, 1946, 1947, 1950, 1965; Sperry & Deupree, 1956; Arora & Sperry, 1957a, 1964)

X-ray CT analysis after blast of composite sandwich panels

Four composite sandwich panels with either single density or graded density foam cores and different face-sheet materials were subjected to full-scale underwater blast testing. The panels were subjected to 1kg PE4 charge at a stand-off distance of 1 m. The panel with graded density core and carbon fiber face-sheets had the lowest deflection. Post-blast damage assessment was carried out using X-ray CT scanning. The damage assessment revealed that there is a trade-off between reduced panel deflection and panel damage. This research has been performed as part of a program sponsored by the Office of Naval Research (ONR)

Myotypic Respecification of Regenerated Nerve-fibres in Cichlid Fishes

In man and other mammals normal motor co-ordination is not restored, as a rule, after regeneration of a severed peripheral nerve-trunk (Sperry, 1945). The random misdirection of regenerating fibres into foreign muscles tends to prevent normal dissociated action within the re-innervated musculature. In contrast, larval amphibians have been found to show excellent recovery of motor function in the form of ‘homologous or myotypic response’ (Weiss, 1936, 1941) following the cutting and regeneration of limb-nerves, limb transplantation, and the cross-connecting of limb nerves to foreign muscles. Similarly, good restoration of muscle co-ordination has been observed in the pectoral fin of adult teleost fishes (Sperry, 1950, 1956)

Evaluating Primary Blast Effects In Vitro

Exposure to blast events can cause severe trauma to vital organs such as the lungs, ears, and brain. Understanding the mechanisms behind such blast-induced injuries is of great importance considering the recent trend towards the use of explosives in modern warfare and terrorist related incidents. To fully understand blast-induced injury, we must first be able to replicate such blast events in a controlled environment using a reproducible method. In this technique using shock tube equipment, shock waves at a range of pressures can be propagated over live cells grown in 2D, and markers of cell viability can be immediately analyzed using a redox indicator assay and the fluorescent imaging of live and dead cells. This method demonstrated that increasing the peak blast overpressure to 127 kPa can stimulate a significant drop in cell viability when compared to untreated controls. Test samples are not limited to adherent cells, but can include cell suspensions, whole-body and tissue samples, through minor modifications to the shock tube setup. Replicating the exact conditions that tissues and cells experience when exposed to a genuine blast event is difficult. Techniques such as the one presented in this article can help to define damage thresholds and identify the transcriptional and epigenetic changes within cells that arise from shock wave exposure

Association of Breakfast Intake with Obesity, Dietary and Physical Activity Behavior Among Urban School-Aged Adolescents in Delhi, India: Results of a Cross-Sectional Study

In developed countries, regular breakfast consumption is inversely associated with excess weight and directly associated with better dietary and improved physical activity behaviors. Our objective was to describe the frequency of breakfast consumption among school-going adolescents in Delhi and evaluate its association with overweight and obesity as well as other dietary, physical activity, and sedentary behaviors. Methods: Design: Cross-sectional study. Setting: Eight schools (Private and Government) of Delhi in the year 2006. Participants: 1814 students from 8th and 10th grades; response rate was 87.2%; 55% were 8th graders, 60% were boys and 52% attended Private schools. Main outcome measures: Body mass index, self-reported breakfast consumption, diet and physical activity related behaviors, and psychosocial factors. Data analysis: Mixed effects regression models were employed, adjusting for age, gender, grade level and school type (SES). Results: Significantly more Government school (lower SES) students consumed breakfast daily as compared to Private school (higher SES) students (73.8% vs. 66.3%; p<0.01). More 8th graders consumed breakfast daily vs. 10th graders (72.3% vs. 67.0%; p<0.05). A dose-response relationship was observed such that overall prevalence of overweight and obesity among adolescents who consumed breakfast daily (14.6%) was significantly lower vs. those who only sometimes (15.2%) or never (22.9%) consumed breakfast (p<0.05 for trend). This relationship was statistically significant for boys (15.4 % vs. 16.5% vs. 26.0; p<0.05 for trend) but not for girls. Intake of dairy products, fruits and vegetables was 5.5 (95% CI 2.4-12.5), 1.7 (95% CI 1.1-2.5) and 2.2 (95% CI 1.3-3.5) times higher among those who consumed breakfast daily vs. those who never consumed breakfast. Breakfast consumption was associated with greater physical activity vs. those who never consumed breakfast. Positive values and beliefs about healthy eating; body image satisfaction; and positive peer and parental influence were positively associated with daily breakfast consumption, while depression was negatively associated. Conclusion: Daily breakfast consumption is associated with less overweight and obesity and with healthier dietary-and physical activity-related behaviors among urban Indian students. Although prospective studies should confirm the present results, intervention programs to prevent or treat childhood obesity in India should consider emphasizing regular breakfast consumption.Obesity Prevention Center, University of MinnesotaPRIME program of the University of Texas, School of Public Health (Stigler, PI)Center for Health Promotion and Disease Prevention Research in Underserved Population

Sandwich Panel Cores for Blast Applications: Materials and Graded Density

Sandwich composites are of interest in marine applications due to their high strength-to-weight ratio and tailorable mechanical properties, but their resistance to air blast loading is not well understood. Full-scale 100 kg TNT equivalent air blast testing at a 15 m stand-off distance was performed on glass-fibre reinforced polymer (GFRP) sandwich panels with polyvinyl chloride (PVC); polymethacrylimid (PMI); and styrene acrylonitrile (SAN) foam cores, all possessing the same thickness and density. Further testing was performed to assess the blast resistance of a sandwich panel containing a stepwise graded density SAN foam core, increasing in density away from the blast facing side. Finally a sandwich panel containing compliant polypropylene (PP) fibres within the GFRP front face-sheet, was subjected to blast loading with the intention of preventing front face-sheet cracking during blast. Measurements of the sandwich panel responses were made using high-speed digital image correlation (DIC), and post-blast damage was assessed by sectioning the sandwich panels and mapping the damage observed. It was concluded that all cores are effective in improving blast tolerance and that the SAN core was the most blast tolerant out of the three foam polymer types, with the DIC results showing a lower deflection measured during blast, and post-blast visual inspections showing less damage suffered. By grading the density of the core it was found that through thickness crack propagation was mitigated, as well as damage in the higher density foam layers, thus resulting in a smoother back face-sheet deflection profile. By incorporating compliant PP fibres into the front face-sheet, cracking was prevented in the GFRP, despite damage being present in the core and the interfaces between the core and face-sheets

Influence of cholesterol and ergosterol on membrane dynamics: a fluorescence approach

Sterols are essential membrane components of eukaryotic cells and are important for membrane organization and function. Cholesterol is the most representative sterol present in higher eukaryotes. It is often found distributed non-randomly in domains or pools in biological and model membranes. Cholesterol-rich functional microdomains (lipid rafts) are often implicated in cell signaling and membrane traffic. Interestingly, lipid rafts have also recently been isolated from organisms such as yeast and Drosophila, which have ergosterol as their major sterol component. Although detailed biophysical characterization of the effect of cholesterol on membranes is well documented, the effect of ergosterol on the organization and dynamics of membranes is not very clear. We have monitored the effect of cholesterol and ergosterol on the dynamic properties of both fluid (POPC) and gel (DPPC) phase membranes utilizing the environment-sensitive fluorescent membrane probe DPH. Our results from steady state and time-resolved fluorescence measurements show, for the first time, differential effects of ergosterol and cholesterol toward membrane organization. These novel results are relevant in the context of lipid rafts in ergosterol-containing organisms such as Drosophila which maintain a low level of sterol compared to higher eukaryotes

Electromagnetic Determination of Carotid Blood Flow in the Anesthetized Rat

The paper describes the design and performance of the smallest iron core electromagnetic flow transducer built so far. The design is miniaturized as to the total volume of the device as well as to the size of the artery it accommodates. The use of this transducer for recording of blood flow in the smallest species of animal employed until now in blood flow research is described. Illustrations of pharmacological observations in anesthetized rats are presented