8 research outputs found

    Atividade antimicrobiana de formulados a base de Alecrim contra Xanthomonas Campestris PV. Campestris e alternaria brassicae / Antimicrobial activity of rosemary based formulates on Xanthomonas Campestris PV. Campestris and alternaria brassicae

    Get PDF
    Considerando a demanda por produtos fitossanitários alternativos aos tradicionais pesticidas, o objetivo deste trabalho foi avaliar a atividade antimicrobiana de formulados orgânico e químico a base de alecrim (Rosmarinus officinalis) sobre Xanthomonas campestris pv. campestris (Xcc) e Alternaria brassicae isolados de couve folha. A atividade antibacteriana in vitro foi realizada a partir de colônia jovens (com 32 h) de Xcc preparada em suspensão 1x108 UFC mL-1 com solução salina (0,85% de NaCl) estéril em tubos de ensaio contendo 5 mL do meio de cultura caldo nutriente, acrescido dos tratamentos 0 (meio de cultura); 0,5%; 1%; 2%; 4% e 6% (massa/volume), avaliando-se a absorbância a 580 nm. A atividade antifúngica in vitro foi feita em meio de cultura suco V8-ágar, esterilizado em autoclave e vertido em placas de Petri, avaliando-se diâmetro das colônias e esporulação, bem como a germinação de esporos em lâminas de microscopia recobertas com ágar-água 1%. Os formulados químico e orgânico reduziram o crescimento de Xcc em 74,5% e 54,8%, respectivamente. Houve inibição do crescimento micelial, esporulação e germinação de esporos de A. brassicae em todas as concentrações testadas de maneira dose-dependente. Houve inibição do crescimento micelial em 40% e 33%, esporulação em 61,35% e 54,5% e na germinação de conídios em 77,41% e 68,22% no formulado químico e orgânico, respectivamente, na concentração 6%. Esses resultados indicam o potencial antimicrobiano dos formulados sobre X. campestris pv. campestris e A. brassicae

    Adubação orgânica e mineral no crescimento inicial de mudas de Cedrela Fissilis Vellozo / Organic and mineral fertilization in the initial growth Cedrela Fissilis Vellozo seedlings

    Get PDF
    O cedro rosa (Cedrela fissilis Vellozo) uma espécie florestal, indicada para ações como reflorestamento, preservação ambiental, arborização urbana, paisagismo, porém, de difícil manejo devido à dificuldade na produção de mudas devido a incidência de doenças destacando-se a morte do ponteiro, dificultando assim a produção de suas mudas como o plantio comercial ou não da espécie, apresenta elevado valor econômico devida as características da sua madeira. Este estudo tem como objetivo avaliar o crescimento e a qualidade das mudas de Cedro Rosa a partir da incorporação do substrato cama aviaria e NPK (9-28-20). O experimento foi conduzido, durante o período de julho de 2019 a fevereiro de 2020, na Universidade Federal Rural da Amazônia (UFRA), Campus Capitão Poço-PA. O delineamento experimental utilizado foi em blocos casualizados (DBC) com 8 (oitro) tratamentos e 6 (seis) repetições, totalizando 48 unidades experimentais obedecendo ao princípio da casualidade. Os tratamentos consistiram na testemunha, somente solo; e somente solo + NPK; diferentes níveis de cama aviária (10%, 20% e 40%) e a combinação de cama aviária + NPK (10%, 20% e 40%). As avaliações foram divididas em análises quantitativas: altura das mudas (AM); diâmetro do coleto (DC); número de folhas (NF); massas secas de folhas (MSF), caule (MSC) e raízes (MSR) e o índice de qualidade de Dickson (IQD), mensuradas ao final do experimento, aos 138 dias após o plantio. Os dados foram submetidos aos pressupostos de normalidade e homoscedasticidade, quando atendidos, foram submetidos à análise de variância com a aplicação do teste F (p < 0,05) e, posteriormente ao teste de médias Tukey (p < 0,05). Observa-se que a utilização de cama aviária 10% + NPK destacou-se apresentando as maiores medias, apesar do substrato cama aviária 10% apresentar resultados semelhantes. Com base nas informações, recomenda-se a utilização de cama aviária 10% do volume total ou cama aviária 10% + NPK incorporadas ao solo. 

    INTERCROPPING OF CARROT X COWPEA-VEGETABLES: EVALUATION OF CULTIVAR COMBINATIONS FERTILIZED WITH ROOSTERTREE

    No full text
    This study aimed to evaluate combinations of cowpea-vegetable cultivars with carrot cultivars in a strip-intercropping system fertilized with roostertree. The work was conducted at the Experimental Farm "Rafael Fernandes" of the Universidade Federal Rural do Semi-Árido (UFERSA) during September 2013 to March 2014. The experimental design was a randomized complete block with four replications and treatments arranged in a factorial 4 x 2 scheme, resulting from the combination of four cowpea -vegetable cultivars (BRS Tumucumaque, BRS Cauamé, BRS Guariba and BRS Itaim) with two carrot cultivars (Brasilia and Alvorada). The characteristics evaluated in cowpea-vegetables were: number of green pods per area, productivity and dry weight of green pods, number of grains per pod, productivity of grains, weight of 100 grains, and dry weight of green grains. In the carrot cultivars, we evaluated total, commercial, and classified productivity of roots. The cultivar combinations were evaluated in terms of land equivalent ratio, productive efficiency index, score of the canonical variable, as well as indicators of gross and net income, rate of return, profit margin, and modified monetary advantage. The intercropping system using the cultivars BRS Guariba (cowpea) and Alvorada (carrot) achieved highest agronomic/biological efficiency. Highest economic efficiency was achieved with the combination BRS Tumucumaque (cowpea) and Brasilia (carrot)

    First isolation of West Nile virus in Brazil

    Get PDF
    BACKGROUND Serological evidence of West Nile virus (WNV) infection has been reported in different regions of Brazil from equine and human hosts but the virus had never been isolated in the country. OBJECTIVES We sought to identify the viral etiology of equine encephalitis in Espírito Santo state. METHODS We performed viral culture in C6/36 cells, molecular detection of WNV genome, histopathology and immunohistochemistry from horse cerebral tissue. We also carried out sequencing, phylogenetic analysis and molecular clock. FINDINGS Histopathologic analysis from horse cerebral tissue showed injury related to encephalitis and WNV infection was confirmed by immunohistochemistry. The virus was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) from brain tissue and subsequently isolated in C6/36 cells. WNV full-length genome was sequenced showing the isolated strain belongs to lineage 1a. The molecular clock indicated that Brazilian WNV strain share the same common ancestor that were circulating in US during 2002-2005. MAIN CONCLUSIONS Here we report the first isolation of WNV in Brazil from a horse with neurologic disease, which was clustered into lineage 1a with others US WNV strains isolated in beginning of 2000’s decade

    Evaluation of a quality improvement intervention to reduce anastomotic leak following right colectomy (EAGLE): pragmatic, batched stepped-wedge, cluster-randomized trial in 64 countries

    Get PDF
    Background Anastomotic leak affects 8 per cent of patients after right colectomy with a 10-fold increased risk of postoperative death. The EAGLE study aimed to develop and test whether an international, standardized quality improvement intervention could reduce anastomotic leaks. Methods The internationally intended protocol, iteratively co-developed by a multistage Delphi process, comprised an online educational module introducing risk stratification, an intraoperative checklist, and harmonized surgical techniques. Clusters (hospital teams) were randomized to one of three arms with varied sequences of intervention/data collection by a derived stepped-wedge batch design (at least 18 hospital teams per batch). Patients were blinded to the study allocation. Low- and middle-income country enrolment was encouraged. The primary outcome (assessed by intention to treat) was anastomotic leak rate, and subgroup analyses by module completion (at least 80 per cent of surgeons, high engagement; less than 50 per cent, low engagement) were preplanned. Results A total 355 hospital teams registered, with 332 from 64 countries (39.2 per cent low and middle income) included in the final analysis. The online modules were completed by half of the surgeons (2143 of 4411). The primary analysis included 3039 of the 3268 patients recruited (206 patients had no anastomosis and 23 were lost to follow-up), with anastomotic leaks arising before and after the intervention in 10.1 and 9.6 per cent respectively (adjusted OR 0.87, 95 per cent c.i. 0.59 to 1.30; P = 0.498). The proportion of surgeons completing the educational modules was an influence: the leak rate decreased from 12.2 per cent (61 of 500) before intervention to 5.1 per cent (24 of 473) after intervention in high-engagement centres (adjusted OR 0.36, 0.20 to 0.64; P < 0.001), but this was not observed in low-engagement hospitals (8.3 per cent (59 of 714) and 13.8 per cent (61 of 443) respectively; adjusted OR 2.09, 1.31 to 3.31). Conclusion Completion of globally available digital training by engaged teams can alter anastomotic leak rates. Registration number: NCT04270721 (http://www.clinicaltrials.gov)
    corecore