74 research outputs found
IMAGE CYTOMETRY FOR DNA ANALYSIS IN ENDOMETRIAL CARCINOMA CORRELATED WITH OTHER PROGNOSTIC PARAMETERS
We evaluated by static cytometry DNA ploidy parameters in 30 stage I-IV endometrial carcinomas and correlated these data with standard clinical-pathological features and disease-free period. We observed a direct correlation between either non-diploid DNA content and deeper myometrial invasion (p<0.02) or D.I.greater than or equal to 1.2 and M2-M3 tumors (p<0.009). The Kaplan Meier survival curves illustrate a more rapid relapse of disease associated with non-diploidy, high 5cExR, high level of proliferation and D.I.greater than or equal to 1.2. while Cox regression model gave relative hazards for disease recurrence of 4, 6, 3.7 and 2.1 for non-diploidy, D.I.greater than or equal to 1.2, high 5cExR and high level of proliferation respectively. This prospective study confirmed the prognostic value of DNA Index, and its usefulness in clinical practice especially in stage I cases, otherwise characterised by favourable standard prognostic factors, is discussed
Cervical carcinogenesis, bacterial vaginosis, HPV-mRNA test and relapse of CIN2+ after loop electrosurgical excision procedure (LEEP)
OBJECTIVE: The aim of the study
was to evaluate the relationship between bacterial
vaginosis (BV) and relapse of cervical intraepithelial
neoplasia grade 2 or more (CIN2+) after Loop
electrosurgical excision procedure (LEEP).
PATIENTS AND METHODS: One hundred four
patients who underwent LEEP for CIN2+ were followed
up every six months for three years. Fifty-three
were negative for BV and fifty-one were positive.
Each clinical control included Pap test, colposcopy,
Amsel criteria test, HPV-DNA, and HPV-mRNA test.
RESULTS: Patients’ age, presence of BV, positivity
to HPV-DNA and HPV-mRNA tests were analyzed.
The average age of patients was 42.5 ± 8.92 years
(median: 42.5; range from 27 to 58 years). The minimum
follow-up was 6 months and maximum 36
months (average: 22.8 ± 4.53; median: 24). The 10%
of the patients with HPV-mRNA test negative had relapsed,
compared to 45% of patients with HPV-mRNA
test positive. Among the 53 patients without BV
the 20% had relapsed compared with 23% of 51 patients
with diagnosis of BV.
CONCLUSIONS: There is no evidence for higher
percentage of relapse in patients with BV, submitted
to excisional procedure for CIN2+ associated
to HPV-m-RNA test positivity. There is only a correlation
among BV and relapse of CIN2+ lesions after
LEEP
Abnormal "low grade" transformation zone: current diagnostic gold standard
The aim of this work was to examine different methods of investigation in the diagnosis of the abnormal "low grade" transformation zone of the portio. Over a period of one year 41 patients subjected to colposcopic examination underwent exo-endocervical sampling for oncologic evaluation and for detection of viral and bacterial infections (HPV, HSV. adenovirus, mycoplasmas and chlamydia trachomatis), as well as portio biopsy. A 65.8% correlation was found between cytology and the HPV-DNA test results. while histology and the presence of the HPV virus agreed in 51.4% of cases. In those cases in which minimal histological alterations were found (koilocytosis) a high percentage of HPV negativity was found. In discordant negative cytologic tests that were however positive for HPV by PCR. the genotypes identified were always 6 and 11
Biofeedback ed elettrostimolazione funzionale dei pavimento pelvico nel trattamento dell'incontinenza urinaria femminile.
Ed. Luigi Pozz
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