15 research outputs found
Purification and kinetic properties of cardiac calmodulin dependent cyclic nucleotide PDE (PDE-I)
Inhibition of isoenzymes of dog heart phosphodiesterase by blue, yellow and red triazine dyes
Crystallization and Biochemical Characterisation of Simian Immunodeficiency Virus Proteinase Mutant
Use of chemical cleavage to release active HIV-1 proteinase from a fusion protein produced in the form of insoluble inclusion bodies
Protein transduction by lipidic peptide dendrimers
We investigated the potential of a new family of lipidic peptide dendrimers in protein transduction into cultured cells. Dendrimer-protein interaction was determined by gel retardation assays using purified recombinant protein. To assess intracellular protein delivery, two marker proteins were used: recombinant firefly luciferase and a Cy3-labeled monoclonal antibody to the c-myc proto-oncogene. Protein delivery was determined by luciferase assays and fluorescence microscopy, respectively. While there was minimal delivery of luciferase or antibody in the absence of the dendrimers, the latter increased protein delivery substantially. Luciferase delivery was concentration and cell type-dependent; the efficiency of delivery also varied with the number of terminal amino groups on the dendrimers. In previous reports, we showed that these dendrimers could be used for gene and drug delivery; the data we report herein suggest that they may also be capable of intracellular protein delivery. This finding has important implications for the use of these dendrimers in protein therapeutics and vaccinology