PCR-based detection of Babesia bovis and Babesia bigemina in their natural host Boophilus microplus and cattle.

PCR and nested-PCR methods were used to assess the frequency of Babesia bovis and Babesia bigemina infection in Boophilus microplus engorged females and eggs and in cattle reared in an area with endemic babesiosis. Blood and the engorged female ticks were from 27 naturally infested calves and 25 crossbred cows. The frequency of both Babesia species was similar in calves and cows (P>0.05). Babesia bovis was detected in 23 (85.2%) calves and in 25 (100%) cows and B. bigemina was detected in 25 (92.6%) calves and in 21 (84%) cows. Mixed infections with the both Babesia species were identified in 42 animals, 21 in each age category. Of female ticks engorged on calves, 34.9% were negative and single species infection with B. bigemina (56.2%) was significantly more frequent (P0.05) to the frequency of single B. bigemina infection (15.9%). Mixed Babesia infection was lower (P<O.OI) than single species infection in female ticks engorged either in cows (5.7%) or in calves (4.3%). An egg sample from each female was analysed for the presence of Babesia species. Of the egg samples from female ticks infected with B. bovis, 26 (47.3%) were infected while from those from female ticks infected with B. bigemina 141 (76.6%) were infected (P<0.01). The results showed that although the frequency of both species of Babesia was similar in calves and cows, the infectivity of B. bigemina was higher to ticks fed on calves while to those ticks fed on cows the infectivity of both Babesia species was similar

Photosynthetic pigment levels in Macroptilium lathyroides L under water deficit.

Título em espanhol: Contenido de pigmentos fotosinteticos Macroptilium lathyroides L en condiciones de sequia

Preliminary genomic analyses of a brazillian isolate of Haemonchus contortus in a model for monepantel resistance.

Anthelmintic resistance is a worldwide problem in sheep production. Monepantel, the most recent anthelmintic released in market, has some reports of resistance, but it is still the most effective treatment to control gastrointestinal parasites.2 - 7 September 2018

Uso de etefom para aumentar a coloração da epiderme de frutos e antecipar a colheita de maçãs "pink lady®".

O cultivo de maçãs (Malus domestica Borkh.) na região sul do Brasil tem grande relevância. A produção brasileira de maçãs fez uso de 34.664 hectares na safra de 2016 (IBGE, 2016). As cultivares Gala e Fuji e seus clones são responsáveis por mais de 90% da produção de maçãs no Sul do Brasil. Entretanto, existem outras opções. A ?Pink Lady® 12 ? foi desenvolvida na Austrália, na década de 70 e foi obtida do cruzamento entre Golden Delicious e Lady Williams (BRACKMANN et al., 2005) e pode ser utilizada na renovação e diversificação dos pomares. A coloração vermelha da epiderme de maçãs é o atributo de maior importância na qualidade do fruto, sendo afetada positivamente pela aplicação exógena de etileno (STEFFENS; BRACKMANN, 2006). A utilização de etefom (ácido 2-cloroetilfosfônico) em pré-colheita visando antecipar a colheita e/ou melhorar a coloração dos frutos é uma das técnicas mais utilizadas (FIORAVANÇO et al., 2007). O etefom denominado comercialmente como Ethrel é um regulador de crescimento que pertence ao grupo químico do ácido fosfônico (ANDREI, 1990). Nesse sentido, objetivou-se com esse trabalho avaliar o uso de etefom para aumentar a coloração da epiderme e antecipar a colheita de maçãs ?Pink Lady® 23 ?, na região de Vacaria/RS no ciclo produtivo de 2015/2016Resumo 194

Portable VIS-NIR spectrometer for non-destructive determination of quality traits in apples and pears.

The objective of this study was to assess the performance of a handheld VIS-NIR spectrometer for non-destructively quality analysis of apples and pears produced in the Brazilian Semi-arid region

Systemic immune responses associated to Haemonchus contortus resistance in a resistant Brazilian sheep breed (Morada Nova).

Haemonchus contortus infection is the greatest cause of economic losses in sheep production worldwide