Uncovering New Functions for MicroRNAs in \u3cem\u3eCaenorhabditis elegans\u3c/em\u3e

In the race to understand microRNA (miRNA) functions in development and physiology, Caenorhabditis elegans investigators were the first out of the gate with the cloning and analysis of the lin-4 and let-7 miRNAs [1,2]. The starting point of strong, penetrant loss of function phenotypes facilitated these advancements. However, subsequent functional analysis of miRNAs in C. elegans was hampered by the lack of easily observable loss-of-function phenotypes [3]. There are several possible models to account for this observation. First, redundancy between related miRNAs can account for the absence of phenotypes in mutants missing individual miRNA genes [4,5]. Second, miRNAs may also function redundantly with unrelated miRNAs or other regulatory mechanisms. Third, identification of miRNA functions may require the analysis of specific cells during development, assays typically not included in initial broad phenotypic analyses. For example, the lsy-6 miRNA is an essential regulator of a chemosensory neuron cell fate in C. elegans [6]. Such a specialized function would not have been identified in broad phenotypic analyses. Finally, miRNAs may act to ‘fine-tune’ gene expression, to maintain protein levels of targets in an optimal range. Loss of this relatively minor regulatory input by miRNAs would not be expected to result in penetrant, observable defects under normal conditions. Recent work has analyzed the functions of individual miRNAs under conditions of environmental or physiological stress. With these approaches, functions for individual miRNAs, which remain elusive under normal growth conditions, have been uncovered. These stresses can be introduced through genetic mutations, environmental perturbations, or through the normal aging process. These results are consistent with the hypothesis that miRNAs act to ensure the robustness of developmental or physiological pathways [7]

Effective Employee Incentive Plans: Features and Implementation Processes

[Excerpt] This paper will evaluate the effectiveness of broad-based employee incentives, identifying the features of effective plans. For our purposes, “broad-based” is used to signal that more than 50 percent of employees are eligible for this variable pay plan. In addition, the terms “variable pay plan” and “pay for performance” are used interchangeably as they appeared in the original sources

The \u3cem\u3emir-51\u3c/em\u3e Family of MicroRNAs Functions in Diverse Regulatory Pathways in \u3cem\u3eCaenorhbditis elegans\u3c/em\u3e

The mir-51 family of microRNAs (miRNAs) in C. elegans are part of the deeply conserved miR-99/100 family. While loss of all six family members (mir-51-56) in C. elegans results in embryonic lethality, loss of individual mir-51 family members results in a suppression of retarded developmental timing defects associated with the loss of alg-1. The mechanism of this suppression of developmental timing defects is unknown. To address this, we characterized the function of the mir-51 family in the developmental timing pathway. We performed genetic analysis and determined that mir-51 family members regulate the developmental timing pathway in the L2 stage upstream of hbl-1. Loss of the mir-51 family member, mir-52, suppressed retarded developmental timing defects associated with the loss of let-7 family members and lin-46. Enhancement of precocious defects was observed for mutations in lin-14, hbl-1, and mir-48(ve33), but not later acting developmental timing genes. Interestingly, mir-51 family members showed genetic interactions with additional miRNA-regulated pathways, which are regulated by the let-7 and mir-35 family miRNAs, lsy-6, miR-240/786, and miR-1. Loss of mir-52 likely does not suppress miRNA-regulated pathways through an increase in miRNA biogenesis or miRNA activity. We found no increase in the levels of four mature miRNAs, let-7, miR-58, miR-62 or miR-244, in mir-52 or mir-52/53/54/55/56 mutant worms. In addition, we observed no increase in the activity of ectopic lsy-6 in the repression of a downstream target in uterine cells in worms that lack mir-52. We propose that the mir-51 family functions broadly through the regulation of multiple targets, which have not yet been identified, in diverse regulatory pathways in C. elegans

The interpretation of TIROS radiation data for practical use in synoptic weather analysis

Interpretation of TIROS III radiation data to demonstrate practical applications in synoptic weather analyse

High-Speed Rail Projects in the United States: Identifying the Elements of Success-Part 2, MTI 06-03

In August 2005, the Mineta Transportation Institute issued the report, High-Speed Rail Projects in the United States: Identifying the Elements for Success. The report noted that since the 1960s, highspeed ground transportation (HSGT) has “held the promise of fast, convenient, and environmentally sound travel for distances between 40 and 600 miles.” After briefly discussing the different experiences with HSGT between the United States and its Asian and European counterparts, the report proceeded to review three U.S. cases—Florida, California, and the Pacific Northwest—as a means for identifying lessons learned for successfully implementing high-speed rail (HSR) in the United States. This report is, in essence, volume 2 of the previous study. Also using a comparative case study approach, this effort adds to the earlier work with three additional cases—the Chicago Hub, the Keystone Corridor, and the Northeast Corridor (NEC). As with the earlier report, the goal of this study is to identify lessons learned for successfully implementing HSR in the United States. Given the early stages of most of these projects, “success” is defined by whether a given HSR project is still actively pursuing development or funding. However, in the case of the Northeast Corridor, a fuller discussion of success is provided since HSR has been implemented on that corridor for some time now

Food Environments Near Home and School Related to Consumption of Soda and Fast Food

Outlines how retail food environments affect adolescents' consumption of sugar-sweetened beverages and fast food, including county-by-county variations. Discusses implications for health outcomes and policy recommendations improve food environments

Characterization of new surface morphologies in a hydrogen-bonded multilayer system

Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2008.Includes bibliographical references (leaf [15]).This work presents an analysis of surface morphology changes in poly(acrylic acid)/polyacrylamide (PAA/PAAm) hydrogen-bonded multilayers. These changes were induced by immersion of the films in aqueous solutions of poly(allylamine hydrochloride), or PAH, at different levels of pH. Positive charges on PAH are attracted to negative charges on PAA, forming ionic bonds and locally decreasing the hydrophilicity of the multilayer. The degree of ionization for each polyelectrolyte, controlled by the pH of the treatment solution, determines the molecular conformations and the extent of electrostatic interactions. These factors, in turn, determine the resulting morphology of the film. Different surface morphologies appeared in four different pH regimes. Highly acidic solutions retained the film's original smooth surface, but wrinkled, honeycomb, or globular morphologies appeared as the pH increased. The three different surface morphologies correlate with the linear, pearl necklace, and globular conformations of PAH.by Allison Kunz.S.B