Functionalized gold nanorod nanocomposite system to modulate differentiation of human mesenchymal stem cells into neural-like progenitors

A 2D multifunctional nanocomposite system of gold nanorods (AuNRs) was developed. Gold nanorods were functionalized via polyethylene glycol with a terminal amine, and, were characterized using transmission and scanning electron microscopy, ultra violet-visible and X-ray photoelectron spectroscopy, and Zeta-potential. The system was cytocompatible to and maintained the integrity of Schwann cells. The neurogenic potential of adipose tissue – derived human mesenchymal stem cells (hMSCs) was evaluated in vitro. The expression pattern and localization of Vimentin confirmed the mesenchymal origin of cells and tracked morphological changes during differentiation. The expression patterns of S100β and glial fibrillary acidic protein (GFAP), were used as indicator for neural differentiation. Results suggested that this process was enhanced when the cells were seeded on the AuNRs compared to the tissue-culture surface. The present study indicates that the design and the surface properties of the AuNRs enhances neural differentiation of hMSCs and hence, would be beneficial for neural tissue engineering scaffolds

Exosome Traceability and Cell Source Dependence on Composition and Cell-Cell Cross Talk

Exosomes are small vesicles with an average diameter of 100 nm that are produced by many, if not all, cell types. Exosome cargo includes lipids, proteins, and nucleic acids arranged specifically in the endosomes of donor cells. Exosomes can transfer the donor cell components to target cells and can affect cell signaling, proliferation, and differentiation. Important new information about exosomes’ remote communication with other cells is rapidly being accumulated. Recent data indicates that the results of this communication depend on the donor cell type and the environment of the host cell. In the field of cancer research, major questions remain, such as whether tumor cell exosomes are equally taken up by cancer cells and normal cells and whether exosomes secreted by normal cells are specifically taken up by other normal cells or also tumor cells. Furthermore, we do not know how exosome uptake is made selective, how we can trace exosome uptake selectivity, or what the most appropriate methods are to study exosome uptake and selectivity. This review will explain the effect of exosome source and the impact of the donor cell growth environment on tumor and normal cell interaction and communication. The review will also summarize the methods that have been used to label and trace exosomes to date

The Effects of Processing Instruction and Output-Based Activities on Grammar Learning: The Mediating Role of Working Memory

Processing instruction (PI) is an input-based approach to grammar education based on input processing theory, and output is another element needed for second-language acquisition to be successful. This study sought to examine the impact of PI and output-based activities with the mediating role of working memory (WM) capacity on learning English future tense. To this end, 99 subjects with preintermediate command of English based on an Oxford Placement Test were recruited for the study, and they were divided into three groups: PI, output, and control, with 33 learners in each group. Using a reading-span test, it was revealed that 14 subjects in the PI group, 15 participants in the output group, and 13 learners in the comparison group had low WM, while the rest individuals had high WM capacity. Then, a two-way between-group analysis of variance and a Bonferroni adjustment post hoc test were performed. The results of the analyses showed that both PI and output groups outperformed the control group. Additionally, there was no difference in grammar gain between PI and output groups. Besides, learners with high WM outperformed low WM individuals. The pedagogical implication of this research is that PI and output-based activities can assist teachers in implementing effective strategies to raise L2 learners’ knowledge and consciousness

Gold Nanorod Substrate for Rat Fetal Neural Stem Cell Differentiation into Oligodendrocytes

Gold nanorods (AuNRs) have been proposed to promote stem cell differentiation in vitro and in vivo. In this study, we examined a particular type of AuNR in supporting the differentiation of rat fetal neural stem cells (NSCs) into oligodendrocytes (ODCs). AuNRs were synthesized according to the seed-mediated method resulting in nanorods with an aspect ratio of around 3 (~12 nm diameter, 36 nm length) and plasmon resonance at 520 and 780 nm, as confirmed by transmission electron microscopy (TEM) and UV-vis spectroscopy, respectively. A layer-by-layer approach was used to fabricate the AuNR substrate on the functionalized glass coverslips. NSCs were propagated for 10 days using fibroblast growth factor, platelet-derived growth-factor-supplemented culture media, and differentiated on an AuNR or poly-D-lysine (PDL)-coated surface using differentiation media containing triiodothyronine for three weeks. Results showed that NSCs survived better and differentiated faster on the AuNRs compared to the PDL surface. By week 1, almost all cells had differentiated on the AuNR substrate, whereas only ~60% differentiated on the PDL surface, with similar percentages of ODCs and astrocytes. This study indicates that functionalized AuNR substrate does promote NSC differentiation and could be a viable tool for tissue engineering to support the differentiation of stem cells

Cut-off low systems over Iraq: Contribution to annual precipitation and synoptic analysis of extreme events

19 pages, 14 figures, 1 table, supporting information https://doi.org/10.1002/joc.6247We combine daily in situ precipitation data with meteorological reanalysis data in order to explore the contribution of cut-off low systems to the seasonal and inter-annual rainfall variations over Baghdad from 2005 to 2016. During these 12 years (average rainfall of 131 ± 67 mm/year), 38 rainy cut-off lows brought 43% of the total precipitation, with extreme inter-annual variations. Indeed, precipitation associated with autumn cut-off lows was the principal factor that turned an arid into a wet year: during the three most arid years cut-off lows contributed about 25% of the average rainfall (10 out of 40 mm/year) while during the three wettest years they contributed near 67% (171 out of 254 mm/year). The extreme-rain cut-off low systems displayed analogous synoptic characteristics: upper-atmosphere divergence, upwards vertical motions in the middle atmosphere, and lower-atmosphere winds into central Iraq at times when the surface Red Sea and Persian Gulf waters were warmer than the surface air. During those days previous to an extreme event, the surface waters cooled substantially and the amount of precipitable water increased largely, suggesting high latent heat transfer. In order to characterize those conditions that favour rainfall, we focus on the November 18–20, 2013 cut-off low system, which led to the largest flooding and wettest year in Baghdad between 2005 and 2016. The distribution of properties in the middle (500 hPa) and upper (250 hPa) troposphere shows that the region was affected by intense horizontal divergence and upwards motions, coinciding with a surface low over the Arabian Peninsula that caused intense northwards winds over the Persian Gulf and brought substantial moisture to central Iraq. The analysis of several stability indexes indicates that convective instability played a secondary role during the episode.This work has been supported by projects VA‐DE‐RETRO (CTM2014‐56987‐P), FLUXES (CTM2015‐69392‐C3‐3‐R), TECBIOMET (CTM2017‐89565‐C2‐1‐P), and SAGA (RTI2018‐100844‐B‐C33), funded by the Spanish government. Ali Raheem Al‐Nassar wishes to thank the Iraqi Ministry of Higher Education and Scientific Research and the Mustansiriyah University for their support to carry out this researchWith the funding support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), of the Spanish Research Agency (AEI