18 research outputs found
Collaboration Networks in the Brazilian Scientific Output in Evolutionary Biology: 2000-2012
Scientific authorships and collaboration network analysis on Chagas disease: papers indexed in PubMed (1940-2009)
Insights into the prevalence and underlying causes of clonal variation through transcriptomic analysis in Pichia pastoris
Investigating the physiological response of Pichia (Komagataella) pastoris GS115 to the heterologous expression of misfolded proteins using chemostat cultures
Guidelines for Small-Scale Production and Purification of Hepatitis B Surface Antigen Virus-Like Particles from Recombinant Pichia pastoris.
Virus-like particle (VLP)-based vaccines have been in the market since decades
for preventing viral infection and have proven their usefulness also in other
areas of biotechnology. Here, we describe in detail simple small-scale production
and purification procedures for the generation of hepatitis B surface antigen
(HBsAg) VLPs using Pichia pastoris as expression host. This protocol may also be
applicable with variations to other HBsAg-based VLPs additionally carrying
antigens of other pathogens