The Thermoanaerobacter Glycobiome Reveals Mechanisms of Pentose and Hexose Co-Utilization in Bacteria

Author Summary Renewable liquid fuels derived from lignocellulosic biomass could alleviate global energy shortage and climate change. Cellulose and hemicellulose are the main components of lignocellulosic biomass. Therefore, the ability to simultaneously utilize pentose and hexose (i.e., co-utilization) has been a crucial challenge for industrial microbes producing lignocellulosic biofuels. Certain thermoanaerobic bacteria demonstrate this unusual talent, but the genetic foundation and molecular mechanism of this process remain unknown. In this study, we reconstructed the structure and dynamics of the first genome-wide carbon utilization network of thermoanaerobes. This transcriptome-based co-expression network reveals that glucose, xylose, fructose, and cellobiose catabolism are each featured on distinct functional modules. Furthermore, the dynamics of the network suggests a distinct yet collaborative nature between glucose and xylose catabolism. In addition, we experimentally demonstrated that these novel network-derived features can be rationally exploited for product-yield enhancement via optimized timing and balanced loading of the carbon supply in a substrate-specific manner. Thus, the newly discovered modular and precisely regulated network elucidates unique features of thermoanaerobic glycobiomes and reveals novel perturbation strategies and targets for the enhanced thermophilic production of lignocellulosic biofuels.Yeshttp://www.plosgenetics.org/static/editorial#pee

Antecedents and Consequences of Marital Satisfaction in an Emirati Sample: A Structural Equation Model Analysis

© 2017 Taylor & Francis. This study surveyed 1,000 married Emirati men and women to explore a model of marital satisfaction in relation to self-esteem, satisfaction with life, gender-role attitudes, and general health (physical and psychological). The Emirati Marital Satisfaction Scale (EMSS), State Self-Esteem Scale (SSES), Satisfaction With Life Scale (SWLS), the Arabic General Health Questionnaire, and the Sex-Role Egalitarianism Scale were used. Confirmatory factor analysis (CFA) on the EMSS indicated a moderate fit of the data to one factor structure. CFA on SSES and SWLS indicated an adequate fit of the data to the four-factor solution and one factor, respectively. The structural equation model for the EMSS showed that both general health and gender-role attitudes predict marital satisfaction and that marital satisfaction seems to predict the individual’s well-being as measured by life satisfaction and self-esteem. These results are discussed within the context of the United Arab Emirates culture and directions for future research are provided