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Not AvailableUnderstanding the genetic diversity among aromatic rice local land races would be useful for their effective utilization in breeding programmes aimed at rice grain quality improvement. The study was conducted to analyse the genetic diversity among 16 aromatic short grain local land races collected from various parts of India and 30 basmati local land races collected from the traditional basmati growing areas, using 26 hyper-variable simple sequence repeat markers spanning the rice genome. Both the aromatic groups could be discriminated by either single (RM28102) or combination of two (RM577 + RM30) markers. Based on the similarity co-efficient values, genotypes were classified into two major clusters with 70% dissimilarity revealing presence of high diversity. The information generated from the present study would be useful in genetic purity assessment, varietal identification for plant variety protection, mapping of genes and background selection for recipient genome recovery in marker-aided breeding programmes aimed at aromatic rice improvemenNot Availabl

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Not AvailableFragrance development in rice has been reported due to a 8-bp deletion in the exon 7 of badh2 gene located on Chromosome 8S. Multiplex markers targeting the functional InDel polymorphism was earlier reported for genotyping fragrance trait, but the marker was observed to be inconsistent and difficult to use. We have developed a simple, co-dominant, functional marker for fragrance trait, which can be resolved in an agarose gel and validated in Basmati and non-Basmati aromatic rice varieties and in a mapping population segregated for fragrance trait. The marker targets the InDel polymorphism in badh2 gene and amplifies 95 and 103 bp fragments in fragrant and non-fragrant genotypes, respectively. The newly developed marker was highly efficient in discriminating all fragrant and non-fragrant genotypes and showed perfect co-segregation with the trait of fragrance in the mapping population. We recommend the use of this simple, low-cost marker in routine genotyping for fragrance trait in large scale breeding materials and germplasm.Department of Biotechnology, Government of IndiaIndian Council of Agricultural Researc

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Not AvailableKernel length in rice (Oryza sativa L.) is controlled by various quantitative trait loci of which GS3 is the most important, being responsible for 80–90% of the variation in kernel length. A mutation in the second exon of this gene has been reported to be associated with maximum variations in the kernel length. We have developed a simple PCR-based marker system named DRR-GL which targets the functional nucleotide polymorphism at GS3. This marker system has the advantages that it is easy to use, saves time and cost, and is amenable for large-scale marker-assisted selection for the trait of kernel length. Validation of this marker in a segregating population and 152 rice varieties, which includes 30 elite basmati varieties, reveals its effective co-segregation and association with the traits of kernel length as well as kernel elongation after cooking. We recommend utilization of this simple, low-cost marker system in breeding programs targeted at improvement of key rice grain quality traits, kernel length and kernel elongation.Not Availabl