75 research outputs found
Mechanism Clarification and Development of Preventive Techniques in Postharvest Rapid Softening of âTonewaseâ Japanese Persimmon Fruit
ãField grown âTonewaseâ Japanese persimmon had been
cultivated as a leading persimmon variety in Wakayama
Prefecture without any problem until 1993. Thereafter,
however, a rapid fruit softening during postharvest distribution occurred and its rate increased every year coinciding with retardation of the temperature decrease in September which is the ripening season for the fruit. This produced a serious loss to the Prefecture. We clarified the mechanism of this fruit softening and developed a completely suppressible technique based on that mechanism.
ãIn âTonewaseâ persimmon fruit, water stress acted as a
primary signal that triggered stress-ethylene production in
the tissue of calyx through stimulating the expression of one
of the ACC synthase genes, DK-ACS2, a stress responsible
ethylene biosynthetic gene. This ethylene diffused into pulp
tissue of the fruit, where transcription of DK-ACS1 for
ripening-ethylene production was activaled through a positive
feedback system, which in turn caused rapid fruit
softening.
ãThis fruit softening derived from stress-ethylene was
completely suppressed by packaging the fruit in perforated
polyethylene bags or by using carton boxes laminated with
a thin layer of plastic film. These techniques that alleviated water loss from fruit completely solved the rapid softening problem during postharvest distribution in âTonewaseâ fruit
Physiological Aspects of Strawberries during Maturation in Relation to Cultivating Conditions, and during Ripening off the Vine
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Automated System for Determining Respiration in Fruits and Vegetables and Regulating Their Temperature by Microcomputer
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Changes of Respiration Rate in Several Kinds of Vegetables under Fluctuating Temperatures
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Changes in Fruit Temperature and Air Constituents in Corrugated Carton by Vibration
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Ripening Characteristics of 'Latundan' Bananas (Musa AAB group) Imported from the Philippines
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Effect of Cultivating Conditions and Postharvest Stepwise Cooling on the Chilling Sensitivity of Eggplant and Cucumber Fruits
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Effects of Accelerated or Delayed Ripening on Hardness, Sugar Content and Acidity of Bananas
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Sugar and Organic Acid Contents in the Strawberry Fruit under Different Cultivating Conditions
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In orader to clarify the differences in regulatory mechanism(s) of the expression of 1-aminocyclopropane-1-carboxylate(ACC) synthase(ACS)genes during ripening in climacteric melon fruit and non-climacteric cucumber fruit, two sets of their genomic DNA sequences, including ca. 2kb of the promoter regions were determined, using PCR-based methods. ACS genes from melon (CMe-ACS1,2) were structurally similar to their counterpart from cucumber (CS-ACS1,2) in terms of size and position of exons and introns, restriction map, and sequencd identity of exeons, introns, proximal 5'-flanking promoter regions and splice junction. Southern blot analysis indicated that each ACS gene is present as a single copy. Transient promoter activity was investigated with two constructs of promoter-β-glucuronidase (GUS) fusion, CMe-ACS1:GUS and CS-ACS1:GUS, in mature mesocarp tissue of the two fruits. In melon disks, GUS activities conferred by the promoters of both CS-ACS1 (-2098ïœ+42) and CMe-ACS-1(-2187ïœ+67) were detected, which were decreased by treatment with 1-methylcyclopropene(1-MCP), an ethylene action inhibitor. In cucumber disks, however, only CS-ACS1:GUS was expressed; the activity was decreased with 1-MCP, and it was not affected by propylene. These results suggest that the promoter of CS-ACS1 has a potential to be expressed in the mesocarp tissue of ripening melon fruit, and that the difference in ethylene biosynthesis between melon and cucumber during ripening may be due to the difference in capability of forming trans-acting factor(s), not due to their ACS1 promoter activities.ã¯ã©ã€ãã¯ããªãã¯åæå®ã®ã¡ãã³ãšãã³ã¯ã©ã€ãã¯ããªãã¯åæå®ã®ãã¥ãŠãªã®æå®è¿œçã«äŒŽãïŒ-ã¢ã
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