B-SIDH: supersingular isogeny Diffie-Hellman using twisted torsion

This paper explores a new way of instantiating isogeny-based cryptography in which parties can work in both the (p+1)-torsion of a set of supersingular curves and in the (p-1)-torsion corresponding to the set of their quadratic twists. Although the isomorphism between a given supersingular curve and its quadratic twist is not defined over GF(p^2) in general, restricting operations to the x-lines of both sets of twists allows all arithmetic to be carried out over GF(p^2) as usual. Furthermore, since supersingular twists always have the same GF(p^2)-rational j-invariant, the SIDH protocol remains unchanged when Alice and Bob are free to work in both sets of twists. This framework lifts the restrictions on the shapes of the underlying prime fields originally imposed by Jao and De Feo, and allows a range of new options for instantiating isogeny-based public key cryptography. These include alternatives that exploit Mersenne and Montgomery-friendly primes, as well as the possibility of significantly reducing the size of the primes in the Jao-De Feo construction at no known loss of asymptotic security. For a given target security level, the resulting public keys are smaller than the public keys of all of the key encapsulation schemes currently under consideration in the NIST post-quantum standardisation effort. The best known attacks against the instantiations proposed in this paper are the classical path finding algorithm due to Delfs and Galbraith and its quantum adapation due to Biasse, Jao and Sankar; these run in respective time O(p^(1/2)) and O(p^(1/4)), and are essentially memory-free. The upshot is that removing the big-O\u27s and obtaining concrete security estimates is a matter of costing the circuits needed to implement the corresponding isogeny. In contrast to other post-quantum proposals, this makes the security analysis of B-SIDH rather straightforward. Searches for friendly parameters are used to find several primes that range from 237 to 256 bits, the conjectured security of which are comparable to the 434-bit prime used to target NIST level 1 security in the SIKE proposal. One noteworthy example is a 247-bit prime for which Alice\u27s secret isogeny is 7901-smooth and Bob\u27s secret isogeny is 7621-smooth

Acquired Type III Secretion System Determines Environmental Fitness of Epidemic Vibrio parahaemolyticus in the Interaction with Bacterivorous Protists

Genome analyses of marine microbial communities have revealed the widespread occurrence of genomic islands (GIs), many of which encode for protein secretion machineries described in the context of bacteria-eukaryote interactions. Yet experimental support for the specific roles of such GIs in aquatic community interactions remains scarce. Here, we test for the contribution of type III secretion systems (T3SS) to the environmental fitness of epidemic Vibrio parahaemolyticus. Comparisons of V. parahaemolyticus wild types and T3SS-defective mutants demonstrate that the T3SS encoded on genome island VPaI-7 (T3SS-2) promotes survival of V. parahaemolyticus in the interaction with diverse protist taxa. Enhanced persistence was found to be due to T3SS-2 mediated cytotoxicity and facultative parasitism of V. parahaemolyticus on coexisting protists. Growth in the presence of bacterivorous protists and the T3SS-2 genotype showed a strong correlation across environmental and clinical isolates of V. parahaemolyticus. Short-term microcosm experiments provide evidence that protistan hosts facilitate the invasion of T3SS-2 positive V. parahaemolyticus into a coastal plankton community, and that water temperature and productivity further promote enhanced survival of T3SS-2 positive V. parahaemolyticus. This study is the first to describe the fitness advantage of GI-encoded functions in a microbial food web, which may provide a mechanistic explanation for the global spread and the seasonal dynamics of V. parahaemolyticus pathotypes, including the pandemic serotype cluster O3:K6, in aquatic environments

Fungal chitinases: diversity, mechanistic properties and biotechnological potential

Chitin derivatives, chitosan and substituted chito-oligosaccharides have a wide spectrum of applications ranging from medicine to cosmetics and dietary supplements. With advancing knowledge about the substrate-binding properties of chitinases, enzyme-based production of these biotechnologically relevant sugars from biological resources is becoming increasingly interesting. Fungi have high numbers of glycoside hydrolase family 18 chitinases with different substrate-binding site architectures. As presented in this review, the large diversity of fungal chitinases is an interesting starting point for protein engineering. In this review, recent data about the architecture of the substrate-binding clefts of fungal chitinases, in connection with their hydrolytic and transglycolytic abilities, and the development of chitinase inhibitors are summarized. Furthermore, the biological functions of chitinases, chitin and chitosan utilization by fungi, and the effects of these aspects on biotechnological applications, including protein overexpression and autolysis during industrial processes, are discussed in this review

In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens

The aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B1 (AFB1) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB1 concentrations in 5% CO2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays were performed. Cell viability decreased in the presence of 10 µg/mL AFB1 at 48 h (p < 0.05) and of 10 and 20 µg/mL AFB1 at 72 h (p < 0.01 and p < 0.001, respectively) when compared to the control (0 µg/mL). However, a dose-dependent increase in the cell-free DNA at 24 h was observed at 1, 10 and 20 µg/mL (p < 0.001). ROS formation significantly increased at 24 h at all concentrations (p < 0.001). The in-vitro results demonstrate that AFB1 is cytotoxic and causes biomolecular oxidative damage in broiler lymphocytes