38 research outputs found
Ten years of a multidisciplinary diabetic foot team approach in Sao Paulo, Brazil
Diabetes mellitus can cause devastating foot problems including loss of protective sensation with subsequent ulcerations and amputations. The natural history and pathophysiology of diabetic foot ulcers is best understood and managed by a multiprofessional team approach. The main factors for prevention and treatment of these devastating diabetic foot conditions are shown, with special attention to education of the patient. This approach decreases the morbidity of the disease, besides its economical and social feasibility
The route to transcription initiation determines the mode of transcriptional bursting in E. coli
Transcription is fundamentally noisy, leading to significant heterogeneity across bacterial populations. Noise is often attributed to burstiness, but the underlying mechanisms and their dependence on the mode of promotor regulation remain unclear. Here, we measure E. coli single cell mRNA levels for two stress responses that depend on bacterial sigma factors with different mode of transcription initiation (Ï70 and Ï54). By fitting a stochastic model to the observed mRNA distributions, we show that the transition from low to high expression of the Ï70-controlled stress response is regulated via the burst size, while that of the Ï54-controlled stress response is regulated via the burst frequency. Therefore, transcription initiation involving Ï54 differs from other bacterial systems, and yields bursting kinetics characteristic of eukaryotic systems