45 research outputs found
A study of the user's perception of economic value in nursing visits to primary care by the method of contingent valuation
Shuttling glucose across brain microvessels, with a little help from GLUT1 and AMP kinase
Galactosylated iron oxide nanoparticles for enhancing oral bioavailability of ceftriaxone
Tyrosinase-catalyzed site-specific immobilization of engineered C-phycocyanin to surface
Enzymatic crosslinking of proteins is often limited by the steric availability of the target residues, as of tyrosyl side chains in the case of tyrosinase. Carrying an N-terminal peptide-tag containing two tyrosine residues, the fluorescent protein C-phycocyanin HisCPC from Synechocystis sp. PCC6803 was crosslinked to fluorescent high-molecular weight forms with tyrosinase. Crosslinking with tyrosinase in the presence of L-tyrosine produced non fluorescent high-molecular weight products. Incubated in the presence of tyrosinase, HisCPC could also be immobilized to amino-modified polystyrene beads thus conferring a blue fluorescence. Crosslinking and immobilization were site-specific as both processes required the presence of the N-terminal peptide in HisCPC