Weekday and weekend days correlates of sedentary time and screen-Based behaviors in children

The aim of this study was to compare weekday and weekend day correlates of sedentary time, as well as some specific screen-based behaviors, in a sample of 213 Spanish six to eleven year-olds (8.68 +/- 1.75 years), 76 boys (8.79 +/- 1.75 years) and 137 girls (8.73 +/- 1.75 years), who wore GT3X accelerometers for 7 days. Screen-based behaviors were reported by parents through questionnaires. Different potential correlates of sedentary time and screen-based behaviors were measured, and data were analyzed using general univariate linear models and multiple regression analysis. Results revealed high levels of screen-based behaviors, both during weekdays and weekend days. From the different significant correlates for each screen-based behavior analyzed, gender, age, hours of extracurricular PA, children''s MVPA and having a TV in the bedroom were identified as the main correlates in most of the behaviors analyzed. The design of multicomponent intervention programs seems advisable. El objetivo de este estudio fue comparar diferentes determinantes de tiempo sedentario en días entre semana y fines de semana, así como determinados comportamientos de consumo de pantallas, en una muestra de 213 niños de 6 a 11 (8.68 ±1.75) años, 76 chicos (8.79 ±1.75) y 137 chicas (8.73±1.75). El tiempo sedentario fue determinado mediante acelerómetros GT3X, que los sujetos llevaron durante 7 días. Los diferentes comportamientos de consumo de pantallas fueron reportados por los padres mediante cuestionarios. Se midieron diferentes potenciales determinantes de tiempo sedentario y consumo de pantallas, y los datos fueron analizados mediante modelos lineales univariantes y análisis de regresión múltiple. Los resultados revelaron altos niveles de consumo de pantallas, tanto en días entre semana como en fines de semana. De los diferentes determinantes para cada tipo de comportamiento de consumo de pantallas analizados, el género, la edad, las horas de AF extracurricular, la AFMV de los niños y tener una TV en el dormitorio, fueron identificados como los principales. El diseño de programas multicomponentes de intervención parece recomendable

Guidance on Dravet Syndrome from Infant to Adult Care: Road Map for Treatment Planning in Europe

Dravet syndrome (DS) is a severe, rare and complex developmental and epileptic encephalopathy affecting 1 in 16'000 live births and characterized by a drug resistant epilepsy, cognitive, psychomotor and language impairment, as well as behavioral disorders. Evidence suggests that optimal treatment of seizures in DS may improve outcomes, even though neurodevelopmental impairments are the likely result of both the underlying genetic variant and the epilepsy. We present an updated guideline for DS diagnosis and treatment, taking into consideration care of the adult patient and non-pharmaceutical therapeutic options for this disease. This up-to-date guideline, which is based on an extensive review of the literature and culminates with a new treatment algorithm for DS, is a European consensus developed through a survey involving 29 European clinical experts in DS. Theis guideline will serve professionals in their clinical practice and, as a consequence, will benefit DS patients and their families

Factors associated with compliance with physical activity recommendations among adolescents in Huesca.

BACKGROUND: Schools have been identified as environments of choice for physical activity promotion. This study examines factors associated with compliance with objectively assessed physical activity recommendations for early adolescents taking part in “Sigue la Huella”, a school-based intervention guided by a social ecological framework and Self-Determination Theory (Deci & Ryan, 2002). METHODS: A total of 200 students (108 boys) aged 12-13 years (M = 12.16; SD = ± 0.51), wore accelerometers during a 7-day period and completed a questionnaire. Participants were considered compliant to the recommendations if their moderate to vigorous physical activity, averaged over 7 days, was =60 minutes a day. RESULTS: 57.4% of boys and 9.9% of girls met recommendations. In a mixed logistic regression model, compliance was higher among boys and students attending private schools, and lower for obese students. Compliance was also associated with higher perceptions of physical competence, higher perceptions of autonomy in physical education, greater importance attached to physical education and less sedentary behavior. CONCLUSIONS: Assessed objectively, gender differences in compliance with physical activity recommendations were greater than expected. Self-Determination Theory emerged as a useful framework to identify motivational factors that can be addressed in school-based physical activity interventions and programs for early adolescents

Importancia del apoyo a las necesidades psicológicas básicas en la predisposición hacia diferentes contenidos en Educación Física.

Siguiendo la teori´a de la autodeterminacio´n, el objetivo de este estudio fue analizar la asociacio´n entre la percepcio´n de apoyo a las necesidades psicolo´gicas ba´sicas (i.e., autonomi´a, competencia y relaciones sociales) y la predisposicio´n hacia el contenido (i.e., actitud cognitiva y afectiva) que tiene el alumnado. El estudio fue desarrollado en tres unidades dida´cticas de Educacio´n Fi´sica, donde participaron 77 alumnos en el contenido de fu´tbol sala, 75 en acrosport y 78 en rugby, pertenecientes a 4o de Educacio´n Secundaria Obligatoria. Los resultados de los ana´lisis de correlacio´n y los ana´lisis predictivos sen~alan una relacio´n positiva entre la percepcio´n del apoyo de las tres necesidades psicolo´gicas y la predisposicio´n hacia el contenido que tienen los alumnos, adquiriendo uno u otro mediador ma´s importancia en funcio´n del contenido curricular impartido. En base a ello, se destaca la importancia de apoyar las tres necesidades psicolo´gicas ba´sicas mediante estrategias de intervencio´n que deben ser disen~adas y adaptadas para cada contenido curricular. Todo ello puede redundar en una mayor predisposicio´n del alumnado hacia cada uno de los contenidos curriculares que integran la Educacio´n Fi´sica, suponiendo una mayor adherencia hacia la pra´ctica de actividad fi´sica. Grounded on the self- determination theory, the aim of the study was to analyze the association between perceived support of basic psychological needs (i.e., autonomy, competence and relatedness) and predisposition toward the content (i.e., cognitive and affective attitude). The study was developed in three Physical Education teaching units. The sample was comprised of 77 football, 75 acrosport and 78 rugby 4th- year compulsory Secondary Education students. The results of the correlation and predictive analysis indicate a positive relationship between perceived support of the three psychological needs and predisposition to the content. The importance of each psychological need was different depending on the teaching unit. The usefulness of supporting the three basic psychological needs through intervention strategies was highlighted. These strategies should be designed and adapted for any curricular content. This can result in a greater predisposition of students to each of the units that integrate Physical Education. It can also lead to greater adherence to participation in physical activity

Dissemination, implementation, and evaluation of an effective school-based intervention to promote physical activity in adolescents: a study protocol

Adolescents around the world do not engage in sufficient physical activity and the Spanish context is no exception. Understanding the educational context as a complex system, school-based multi-level and multi-component interventions seem to be an effective strategy to reverse this trend. Moreover, a co-creational approach seems to facilitate the mobilization of community partnerships and the engagement of stakeholders in the intervention process. This study aims to describe the dissemination, implementation, and evaluation process of an effective school-based intervention program in another setting using the replicating effective programs framework and a co-participatory approach. This study will be conducted in two Spanish secondary schools located in the region of Aragon (experimental vs. control school) in a sample of adolescents in the second grade (13–14 years old). To evaluate the effectiveness, different health behaviors such as physical activity, sleep, sedentary time with screens, nutrition, and psychosocial variables will be quantitatively measured at baseline and after the implementation of the intervention. Qualitative methods will also be used to better understand the implementation process and the co-creation approach, as well as to provide insights into the sustainability of the intervention program. The current study has the potential to provide strong information about the dissemination, implementation, and evaluation process of school-based programs to promote healthy behaviors among adolescents

Genome-wide profiling of methylation identifies novel targets with aberrant hyper-methylation and reduced expression in low-risk myelodysplastic syndromes

Gene expression profiling signatures may be used to classify the subtypes of Myelodysplastic syndrome (MDS) patients. However, there are few reports on the global methylation status in MDS. The integration of genome-wide epigenetic regulatory marks with gene expression levels would provide additional information regarding the biological differences between MDS and healthy controls. Gene expression and methylation status were measured using high-density microarrays. A total of 552 differentially methylated CpG loci were identified as being present in low-risk MDS; hypermethylated genes were more frequent than hypomethylated genes. In addition, mRNA expression profiling identified 1005 genes that significantly differed between low-risk MDS and the control group. Integrative analysis of the epigenetic and expression profiles revealed that 66.7% of the hypermethylated genes were underexpressed in low-risk MDS cases. Gene network analysis revealed molecular mechanisms associated with the low-risk MDS group, including altered apoptosis pathways. The two key apoptotic genes BCL2 and ETS1 were identified as silenced genes. In addition, the immune response and micro RNA biogenesis were affected by the hypermethylation and underexpression of IL27RA and DICER1. Our integrative analysis revealed that aberrant epigenetic regulation is a hallmark of low-risk MDS patients and could have a central role in these diseases. © 2013 Macmillan Publishers Limited All rights reserved

Development of a novel splice array platform and its application in the identification of alternative splice variants in lung cancer

Abstract Background Microarrays strategies, which allow for the characterization of thousands of alternative splice forms in a single test, can be applied to identify differential alternative splicing events. In this study, a novel splice array approach was developed, including the design of a high-density oligonucleotide array, a labeling procedure, and an algorithm to identify splice events. Results The array consisted of exon probes and thermodynamically balanced junction probes. Suboptimal probes were tagged and considered in the final analysis. An unbiased labeling protocol was developed using random primers. The algorithm used to distinguish changes in expression from changes in splicing was calibrated using internal non-spliced control sequences. The performance of this splice array was validated with artificial constructs for CDC6, VEGF, and PCBP4 isoforms. The platform was then applied to the analysis of differential splice forms in lung cancer samples compared to matched normal lung tissue. Overexpression of splice isoforms was identified for genes encoding CEACAM1, FHL-1, MLPH, and SUSD2. None of these splicing isoforms had been previously associated with lung cancer. Conclusions This methodology enables the detection of alternative splicing events in complex biological samples, providing a powerful tool to identify novel diagnostic and prognostic biomarkers for cancer and other pathologies

Mechanisms of stretch-mediated skin expansion at single-cell resolution.

The ability of the skin to grow in response to stretching has been exploited in reconstructive surgery1. Although the response of epidermal cells to stretching has been studied in vitro2,3, it remains unclear how mechanical forces affect their behaviour in vivo. Here we develop a mouse model in which the consequences of stretching on skin epidermis can be studied at single-cell resolution. Using a multidisciplinary approach that combines clonal analysis with quantitative modelling and single-cell RNA sequencing, we show that stretching induces skin expansion by creating a transient bias in the renewal activity of epidermal stem cells, while a second subpopulation of basal progenitors remains committed to differentiation. Transcriptional and chromatin profiling identifies how cell states and gene-regulatory networks are modulated by stretching. Using pharmacological inhibitors and mouse mutants, we define the step-by-step mechanisms that control stretch-mediated tissue expansion at single-cell resolution in vivo.Wellcome Trust Royal Societ

Global Research Priorities to Better Understand the Burden of Iatrogenic Harm in Primary Care: An International Delphi Exercise

There is a need to identify and reach agreement on key foci for patient safety research in primary care contexts and understand how these priorities differ between low-, middle-, and high-income settings. We conducted a modified Delphi exercise, which was distributed to an international panel of experts in patient safety and primary care. Family practice and pharmacy were considered the main contexts on which to focus attention in order to advance patient safety in primary care across all income categories. Other clinical contexts prioritised included community midwifery and nursing in low-income countries and care homes in high-income countries. The sources of patient safety incidents requiring further study across all economic settings that were identified were communication between health care professionals and with patients, teamwork within the health care team, laboratory and diagnostic imaging investigations, issues relating to data management, transitions between different care settings, and chart/patient record com- pleteness. This work lays the foundation for a range of research initiatives that aim to promote a more comprehensive appreciation of the burden of unsafe primary care, develop understanding of the main areas of risk, and identify interventions that can enhance the safety of primary care provision internationall

A Systematic Literature Review with Meta-Analyses of Within- and Between-Day Differences in Objectively Measured Physical Activity in School-Aged Children

Background: Targeting specific time periods of the day or week may enhance physical activity (PA) interventions in youth. The most prudent time segments to target are currently unclear.  Objectives: To systematically review the literature describing differences in young people’s objectively measured PA on weekdays vs. weekends, in school vs. out of school, weekends vs. out of school and lesson time vs. break time.  Methods: Electronic databases were searched for English-language, cross-sectional studies of school-aged children (4–18 years) reporting time-segment-specific accelerometer-measured PA from 01/1990 to 01/2013. We meta-analysed standardised mean differences (SMD) between time segments for mean accelerometer counts per minute (TPA) and minutes in moderate-to-vigorous PA (MVPA). SMD is reported in units of standard deviation; 0.2, 0.5 and 0.8 represent small, moderate and large effects. Heterogeneity was explored using meta-regression (potential effect modifiers: age, sex and study setting).  Results: Of the 54 included studies, 37 were eligible for meta-analyses. Children were more active on weekdays than weekends [pooled SMD (95 % CI) TPA 0.14 (0.08; 0.20), MVPA 0.42 (0.35; 0.49)]. On school days, TPA was lower in school than out of school; however, marginally more MVPA was accumulated in school [TPA −0.24 (−0.40; −0.08), MVPA 0.17 (−0.03; 0.38)]. TPA was slightly lower on weekends than out of school on school days, but a greater absolute volume of MVPA was performed on weekends [TPA −0.10 (−0.19; −0.01), MVPA 1.02 (0.82; 1.23)]. Heterogeneity between studies was high (I2 73.3–96.3 %), with 20.3–53.1 % of variance between studies attributable to potential moderating factors.  Conclusions: School-aged children are more active on weekdays than weekend days. The outcome measure influences the conclusions for other comparisons. Findings support the tailoring of intervention strategies to specific time periods