12 research outputs found
Multiple termination sites in an unlinked 5S rRNA operon in the archaebacterium, Thermococcus celer
No full textA yeast ribosomal DNA-binding protein that binds to the rDNA enhancer and also close to the site of Pol I transcription initiation is not important for enhancer functioning
No full textRB in breast cancer: Differential effects in estrogen receptor-positive and estrogen receptor-negative disease: Comment on: Ertel A, et al. Cell Cycle 2010; 9:4153–63.
No full textThe yeast rRNA gene enhancer does not function by recycling RNA polymerase I and cannot act as a UAS
No full textSpecific interaction of the murine transcription termination factor TTF I with class-I RNA polymerases
No full textDNA Elements and Protein Factors Involved in the Regulation of Transcription of the Ribosomal RNA Genes in Yeast
No full text
Epigenetic regulation of TTF-I-mediated promoter–terminator interactions of rRNA genes
No full textRibosomal RNA synthesis is the eukaryotic cell's main transcriptional activity, but little is known about the chromatin domain organization and epigenetics of actively transcribed rRNA genes. Here, we show epigenetic and spatial organization of mouse rRNA genes at the molecular level. TTF-I-binding sites subdivide the rRNA transcription unit into functional chromatin domains and sharply delimit transcription factor occupancy. H2A.Z-containing nucleosomes occupy the spacer promoter next to a newly characterized TTF-I-binding site. The spacer and the promoter proximal TTF-I-binding sites demarcate the enhancer. DNA from both the enhancer and the coding region is hypomethylated in actively transcribed repeats. 3C analysis revealed an interaction between promoter and terminator regions, which brings the beginning and end of active rRNA genes into close contact. Reporter assays show that TTF-I mediates this interaction, thereby linking topology and epigenetic regulation of the rRNA genes
