Controlled Release of Growth Factors on Allograft Bone in vitro

Allografts are important alternatives to autografts for treating defects after major bone loss. Bone growth factors have both local autocrine and paracrine effects and regulate the growth, proliferation, and differentiation of osteoprogenitor cells. To study the effects of prolonged, continuous, local delivery of growth factors on bone growth, we developed a new microelectromechanical system (MEMS) drug delivery device. Bone marrow cells from mice were seeded on mouse allograft discs and cultured in osteogenic media with osteogenic protein 1 (OP-1) and/or basic fibroblast growth factor (FGF-2) delivered from MEMS devices for 6 weeks. We monitored bone formation by changes of bone volume using micro-CT scanning and release of osteocalcin using ELISA. The data suggest the MEMS devices delivered constant concentrations of OP-1 and FGF-2 to the media. Bone marrow cells grew on the allografts and increased bone volume. Addition of OP-1 increased bone formation whereas FGF-2 decreased bone formation. Local delivery of growth factors over a prolonged period modulated the differentiation of osteoprogenitor cells on allograft bone

Porous Silicon Functionalities for BioMEMS

This chapter presents a literature survey of the applications of porous silicon in BioMEMS (biological/biomedical microelectromechanical systems). This material possesses properties particularly suitable for biomedical purposes: biocompatibility, biodegradability, photoluminescence, ability to precisely control the pore size and shape, and possibility to easily modify the surface chemistry. Many applications can, for instance, be found in the fields of sensing and delivery of therapeutics. It is expected that the number of BioMEMS using porous silicon will continue to increase in the future with the development of lab-on-a-chip/ microfluidic devices.SCOPUS: ch.binfo:eu-repo/semantics/publishe

Porous silicon functionalities for BioMEMS

This chapter presents a literature survey of the applications of porous silicon in BioMEMS (biological/biomedical microelectromechanical systems). This material possesses properties particularly suitable for biomedical purposes: biocompatibility, biodegradability, photoluminescence, ability to precisely control the pore size and shape, and possibility to easily modify the surface chemistry. Many applications can, for instance, be found in the fields of sensing and delivery of therapeutics. It is expected that the number of BioMEMS using porous silicon will continue to increase in the future with the development of lab-on-a-chip/ microfluidic devices.SCOPUS: ch.binfo:eu-repo/semantics/publishe