EFFECT OF CONCENTRATIONS AND TYPE OF SALT SOLUTIONS ON THE EXTRACTION OF NITROGENOUS COMPOUNDS FROM PHASEOLUS VULGARIS SEEDS

Effect of different salts, with varying concentrations on the extractability of nitrogenous compounds from three cultivars of Phaseolus vulgaris showed that maximum nitrogen extractability attained by the salts can be arranged on a decreasing order as follows: sodium carbonate, disodium phosphate, sodium citrate, magnesium chloride and sodium sulphate. The amount of nitrogen extracted can be arranged on decreasing order for each variety as follows: Swissblan, Contender and Giza and this agrees with the amount of total nitroten resp. protein in each variety and found to be 25.1%, 23.8% and 21.45%, respectively (NX 6.25 basis)

EXTRACTION, FRACTIONATION AND CHARACTERIZATION OF COWPEA (VIGNA UNGUICULATA) GLOBULINS

The proteins were extracted from cowpea seeds by buffered saline solution and the globulins were separated by dialyzing the protein extract against distilled water. Fractionation of the total globulins on Sephacryl S-200 column produced two major and one minor components. The molecular weight of the major globulin components were estimated to be greater than 250000 and 178 000 for the globulin component (a) and (b) respectively and the latter component was the predominant globulin. The two major globulin components were free from contamination of other proteins as shown by polyacrylamide gel electrophoresis. Spectrophotometry and amino acid analyses were used as tools for characterizing the globulin components