THEORIZING CRITICAL POPULIST DISCOURSE ANALYSIS: A NEW PLAUSIBLE PARADIGM

As deeply related to Critical Theory, CDA has been established to deconstruct the hegemonic objective reasoning of elites and to construct an influential subjective rationality that contributes to generating a free human sense. Though CDA impartially centers on revealing power relations, its agenda is still politically detained.  Discourse- historical approach, for example, has been employed by Wodak (2015) in the analysis of right-wing populist ideology in Europe; it proves to be deconstructive, revealing only those radical discursive strategies existing in the right-wing populist discourses. Thus, it is essential to initiate a new paradigm in CDA which constructs a comprehensive framework that critically studies the different forms of populist discourses through analyzing their innate ideologies, emancipatory tactics, anti-elitist values, and sentimental attitudes toward people. This newly suggested paradigm, namely critical populist discourse analysis (CPDA) is expected to cause a ground-breaking step in critical studies as it provides a critical mapping for the multi arguments in populist discourses. This article, thus aims to argue about this proposed paradigm in CDA that provides a critical account on the insights of populist projects of emancipation. The article also highlights the interest of CPDA in interpreting the transformation of populist discourses from rationalism into radicalism. This suggested paradigm addresses all populist movements in the world, including those in Nusantara territories as CPDA’s main interest is to objectively analyze and value the core concepts of emancipatory discourses. This paradigm is also applicable to analyze the discourses of liberation movements against the colonial power in these territories.  Key words: Critical realism, critical theory, discourse analysis, methods in qualitative inquiry, qualitative evaluation Cite as: Al-Ramahi, R. A. & Ab Rashid, R. (2019). Theorizing critical populist discourse analysis: A new plausible paradigm. Journal of Nusantara Studies, 4(1), 422-446. http://dx.doi.org/10.24200/jonus.vol4iss1pp422-44

Queue backlog as a node metric for RPL protocol

The current de-facto routing protocol over WSN developed by IETF Working Group (6LOWPAN) named as Routing Protocol for Low Power and Lossy networks (RPL) [1], to enable IPv6 packets carrying over IEEE 802.4 and to empower the usage of IoT over WSN. Because of the potential large networks, number of nodes and the fact that multiple coexisting applications will be running in the same physical layer, RPL in the network layer faces throughput challenges. For the purpose of overcoming these problems many researchers focused on multipath solutions including a Back-Pressure routing protocol for data collection called BackIP [2], however it shows a superior throughput performance, BackIP have shortcomings of higher delay and limited applicability. In this paper, we introduce a node metric based on nodes Queue Backlogs for RPL protocol, which leads to better throughput performance while maintaining the delay and the ability to use with the different network applications. This metric depends on the Packet Queue length of the nodes with the consideration of other link and node metrics, like ETX or Energy usage, leading to better load balancing in the network. Moreover we discuss the needed design changes to enable our metric to perform in an efficient way. Keywords-IOT, RPL, WSN, Back Pressure, Queue Backlog, Objective Functio

Caryoscope: An Open Source Java application for viewing microarray data in a genomic context

BACKGROUND: Microarray-based comparative genome hybridization experiments generate data that can be mapped onto the genome. These data are interpreted more easily when represented graphically in a genomic context. RESULTS: We have developed Caryoscope, which is an open source Java application for visualizing microarray data from array comparative genome hybridization experiments in a genomic context. Caryoscope can read General Feature Format files (GFF files), as well as comma- and tab-delimited files, that define the genomic positions of the microarray reporters for which data are obtained. The microarray data can be browsed using an interactive, zoomable interface, which helps users identify regions of chromosomal deletion or amplification. The graphical representation of the data can be exported in a number of graphic formats, including publication-quality formats such as PostScript. CONCLUSION: Caryoscope is a useful tool that can aid in the visualization, exploration and interpretation of microarray data in a genomic context

Chemopreventive potential of β-Sitosterol in experimental colon cancer model - an In vitro and In vivo study

<p>Abstract</p> <p>Background</p> <p><it>Asclepias curassavica </it>Linn. is a traditional medicinal plant used by tribal people in the western ghats, India, to treat piles, gonorrhoea, roundworm infestation and abdominal tumours. We have determined the protective effect of β-sitosterol isolated from <it>A. curassavica </it>in colon cancer, using <it>in vitro </it>and <it>in vivo </it>models.</p> <p>Methods</p> <p>The active molecule was isolated, based upon bioassay guided fractionation, and identified as β-sitosterol on spectral evidence. The ability to induce apoptosis was determined by its <it>in vitro </it>antiradical activity, cytotoxic studies using human colon adenocarcinoma and normal monkey kidney cell lines, and the expression of β-catenin and proliferating cell nuclear antigen (PCNA) in human colon cancer cell lines (COLO 320 DM). The chemopreventive potential of β-sitosterol in colon carcinogenesis was assessed by injecting 1,2-dimethylhydrazine (DMH, 20 mg/kg b.w.) into male Wistar rats and supplementing this with β-sitosterol throughout the experimental period of 16 weeks at 5, 10, and 20 mg/kg b.w.</p> <p>Results</p> <p>β-sitosterol induced significant dose-dependent growth inhibition of COLO 320 DM cells (IC₅₀266.2 μM), induced apoptosis by scavenging reactive oxygen species, and suppressed the expression of β-catenin and PCNA antigens in human colon cancer cells. β-sitosterol supplementation reduced the number of aberrant crypt and crypt multiplicity in DMH-initiated rats in a dose-dependent manner with no toxic effects.</p> <p>Conclusion</p> <p>We found doses of 10-20 mg/kg b.w. β-sitosterol to be effective for future <it>in vivo </it>studies. β-sitosterol had chemopreventive potential by virtue of its radical quenching ability <it>in vitro</it>, with minimal toxicity to normal cells. It also attenuated β-catenin and PCNA expression, making it a potential anticancer drug for colon carcinogenesis.</p

No Forbidden Landscape in String/M-theory

Scale invariant but non-conformal field theories are forbidden in (1+1) dimension, and so should be the corresponding holographic dual gravity theories. We conjecture that such scale invariant but non-conformal field configurations do not exist in the string/M-theory. We provide a proof of this conjecture in the classical supergravity limit under a certain gauge condition. Our proof does also apply in higher dimensional scale invariant but non-conformal field configurations, which suggests that scale invariant but non-conformal field theories may be forbidden in higher dimensions as well.Comment: 14 pages, v2: energy condition on c-theorem is corrected, v3: extra assumption in the proof is discussed due to a sign error in the previous versio

Green extraction of bioactive compounds from plant biomass and their application in meat as natural antioxidant

Plant extracts are rich in various bioactive compounds exerting antioxidants effects, such as phenolics, catechins, flavonoids, quercetin, anthocyanin, tocopherol, rutin, chlorogenic acid, lycopene, caffeic acid, ferulic acid, p-coumaric acid, vitamin C, protocatechuic acid, vitamin E, carotenoids, β-carotene, myricetin, kaempferol, carnosine, zeaxanthin, sesamol, rosmarinic acid, carnosic acid, and carnosol. The extraction processing protocols such as solvent, time, temperature, and plant powder should be optimized to obtain the optimum yield with the maximum concentration of active ingredients. The application of novel green extraction technologies has improved extraction yields with a high concentration of active compounds, heat-labile compounds at a lower environmental cost, in a short duration, and with efficient utilization of the solvent. The application of various combinations of extraction technologies has proved to exert a synergistic effect or to act as an adjunct. There is a need for proper identification, segregation, and purification of the active ingredients in plant extracts for their efficient utilization in the meat industry, as natural antioxidants. The present review has critically analyzed the conventional and green extraction technologies in extracting bioactive compounds from plant biomass and their utilization in meat as natural antioxidants

Integrating teachers’ TPACK levels and students’ learning motivation, technology innovativeness, and optimism in an IoT acceptance model

The growing use of the Internet of Things (IoT) around the world has encouraged researchers to investigate how and why the IoT is implemented in colleges and universities. Previous studies have focused on individual attitudes rather than the integration of attitudes from two different perspectives. Furthermore, other studies have investigated the use of the IoT in non-educational settings, ignoring the effect of the IoT related to the technology acceptance model (TAM) and technological pedagogical content knowledge (TPACK) model. The present work aims to address this research gap by determining the main factors that influence acceptance of the IoT, leading to increased awareness in collaborative learning, where technology forms the core tool in enhancing the use of the IoT. A questionnaire was used to collect data from teachers and students from colleges and universities in Oman and the United Arab Emirates (UAE). The data were analyzed through the structural equation modeling (SEM) method. The findings indicated that there are two levels of positive effects on the intention to use IoT. The first level is technology features, which are represented by technology optimism and technology innovation; these factors are crucial to using the IoT. The second level is learning motivation, which has a close relationship with teachers’ knowledge, and content pedagogy, which has a significant effect on the familiarity with IoT tools and applications. TAM constructs have a positive and direct impact on the intention to use IoT. The practical and managerial implications show that teachers, educators, and students can obtain benefits from these results to help IoT features to suit users’ needs

Determinants influencing the continuous intention to use digital technologies in Higher Education

There is increasing evidence that the lack of access to digital information and technologies is not widely considered in the educational sectors when focusing on the perceived experience, tutor quality and students’ satisfaction. In this paper, we report on an evaluation of a project aiming to bridge the use of digital information in the educational sector by proposing an integrated model that measures teachers’ quality, uncertainty avoidance effects and students’ satisfaction concerning TAM constructs and the perceived experience of digital information in education (DIE). The model and hypotheses were validated using data collected from a survey of 553 students at a college level. The results revealed that users may perceive the importance of DIE based on several external factors that enhance their learning and teaching experiences. The personal characteristics of the user including his/her readiness to use technology are crucial in correlation with the perceived ease of use. In addition, the high quality of the tutor in some cultures may enhance the perceived usefulness of the technology. Other factors such as flow of information, uncertain avoidance and satisfaction may strongly assess the continuous intention to use the technology

Oxygen Tension Is a Determinant of the Matrix-Forming Phenotype of Cultured Human Meniscal Fibrochondrocytes

BACKGROUND: Meniscal cartilage displays a poor repair capacity, especially when injury is located in the avascular region of the tissue. Cell-based tissue engineering strategies to generate functional meniscus substitutes is a promising approach to treat meniscus injuries. Meniscus fibrochondrocytes (MFC) can be used in this approach. However, MFC are unable to retain their phenotype when expanded in culture. In this study, we explored the effect of oxygen tension on MFC expansion and on their matrix-forming phenotype. METHODOLOGY/PRINCIPAL FINDINGS: MFC were isolated from human menisci followed by basic fibroblast growth factor (FGF-2) mediated cell expansion in monolayer culture under normoxia (21%O(2)) or hypoxia (3%O(2)). Normoxia and hypoxia expanded MFC were seeded on to a collagen scaffold. The MFC seeded scaffolds (constructs) were cultured in a serum free chondrogenic medium for 3 weeks under normoxia and hypoxia. Constructs containing normoxia-expanded MFC were subsequently cultured under normoxia while those formed from hypoxia-expanded MFC were subsequently cultured under hypoxia. After 3 weeks of in vitro culture, the constructs were assessed biochemically, histologically and for gene expression via real-time reverse transcription-PCR assays. The results showed that constructs under normoxia produced a matrix with enhanced mRNA ratio (3.5-fold higher; p<0.001) of collagen type II to I. This was confirmed by enhanced deposition of collagen II using immuno-histochemistry. Furthermore, the constructs under hypoxia produced a matrix with higher mRNA ratio of aggrecan to versican (3.5-fold, p<0.05). However, both constructs had the same capacity to produce a glycosaminoglycan (GAG) -specific extracellular matrix. CONCLUSIONS: Our data provide evidence that oxygen tension is a key player in determining the matrix phenotype of cultured MFC. These findings suggest that the use of normal and low oxygen tension during MFC expansion and subsequent neo-tissue formation cultures may be important in engineering different regions of the meniscus

Zoledronic acid treatment impairs protein geranyl-geranylation for biological effects in prostatic cells

BACKGROUND: Nitrogen-containing bisphosphonates (N-BPs) have been designed to inhibit osteoclast-mediated bone resorption. However, it is now accepted that part of their anti-tumor activities is related to interference with the mevalonate pathway. METHODS: We investigated the effects of zoledronic acid (ZOL), on cell proliferation and protein isoprenylation in two tumoral (LnCAP, PC-3,), and one normal established (PNT1-A) prostatic cell line. To assess if inhibition of geranyl-geranylation by ZOL impairs the biological activity of RhoA GTPase, we studied the LPA-induced formation of stress fibers. The inhibitory effect of ZOL on geranyl geranyl transferase I was checked biochemically. Activity of ZOL on cholesterol biosynthesis was determined by measuring the incorporation of (14)C mevalonate in cholesterol. RESULTS: ZOL induced dose-dependent inhibition of proliferation of all the three cell lines although it appeared more efficient on the untransformed PNT1A. Whatever the cell line, 20 μM ZOL-induced inhibition was reversed by geranyl-geraniol (GGOH) but neither by farnesol nor mevalonate. After 48 hours treatment of cells with 20 μM ZOL, geranyl-geranylation of Rap1A was abolished whereas farnesylation of HDJ-2 was unaffected. Inhibition of Rap1A geranyl-geranylation by ZOL was rescued by GGOH and not by FOH. Indeed, as observed with treatment by a geranyl-geranyl transferase inhibitor, treatment of PNT1-A cells with 20 μM ZOL prevented the LPA-induced formation of stress fibers. We checked that in vitro ZOL did not inhibit geranyl-geranyl-transferase I. ZOL strongly inhibited cholesterol biosynthesis up to 24 hours but at 48 hours 90% of this biosynthesis was rescued. CONCLUSION: Although zoledronic acid is currently the most efficient bisphosphonate in metastatic prostate cancer management, its mechanism of action in prostatic cells remains unclear. We suggest in this work that although in first intention ZOL inhibits FPPsynthase its main biological actitivity is directed against protein Geranylgeranylation