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Not AvailableEffect of kind and concentration of culture media, and plant growth regulators on in vitro response was studied in an economically important seaweed species viz. Gracilaria corticata var. cylindrica. Filter sterilized autoclaved artificial seawater medium at 100 % concentration (A2) was found to be the most optimum for in vitro culture as the cultured explants showed superiority in terms of growth and photosynthetic pigment content, apart from inducing lateral bud formation. Incorporation of cytokinin alone or in combination with auxin (IAA) promoted growth of seaweed explants in A2 media.A2 medium supplemented with kinetin at different concentrations showed the highest total chlorophyll content (K2), highest total carotenoid content (K3) and better induction of lateral buds (K1). Hence, considering the promising response of artificial seawater supplemented with cytokinins and auxin for in vitro culture of the species, the present investigation could serve as a base study for formulating future research programmes.Not Availabl