63 research outputs found
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Genotoxic and Anatomical Deteriorations Associated with Potentially Toxic Elements Accumulation in Water Hyacinth Grown in Drainage Water Resources
Potentially toxic elements (PTEs)-induced genotoxicity on aquatic plants is still an open question. Herein, a single clone from a population of water hyacinth covering a large distribution area of Nile River (freshwater) was transplanted in two drainage water resources to explore the hazardous effect of PTEs on molecular, biochemical and anatomical characters of plants compared to those grown in freshwater. Inductivity Coupled Plasma (ICP) analysis indicated that PTEs concentrations in water resources were relatively low in most cases. However, the high tendency of water hyacinth to bio-accumulate and bio-magnify PTEs maximized their concentrations in plant samples (roots in particular). A Random Amplified Polymorphic DNA (RAPD) assay showed the genotoxic effects of PTEs on plants grown in drainage water. PTEs accumulation caused substantial alterations in DNA profiles including the presence or absence of certain bands and even the appearance of new bands. Plants grown in drainage water exhibited several mutations on the electrophoretic profiles and banding pattern of total protein, especially proteins isolated from roots. Several anatomical deteriorations were observed on PTEs-stressed plants including reductions in the thickness of epidermis, cortex and endodermis as well as vascular cylinder diameter. The research findings of this investigation may provide some new insights regarding molecular, biochemical and anatomical responses of water hyacinth grown in drainage water resources.</jats:p
Knowledge and awareness about food safety, foodborne diseases, and microbial hazards: A cross-sectional study among Bangladeshi consumers of street-vended foods
Maintaining quality and safety of street-vended foods (SVFs) is a challenge and a public health priority in low-and middle-income countries due to its affordability, availability and association with foodborne diseases and microbial hazards. The purpose of this study was to assess the knowledge and awareness of food safety, foodborne diseases, and microbial hazards among Bangladeshi consumers of SVFs. A cross-sectional survey was administered among 650 Bangladeshi adults who purchase and consume SVFs. The mean food safety knowledge score of consumers was 10.73 (SD = 2.84, range: 3â18), indicating moderate knowledge. Multiple linear regression analysis found that male consumers (B = -0.549, p < 0.030), consumers with âno formal educationâ (B = â1.815, p < 0.045), and consumers with âsecondary educationâ (B = â1.476, p < 0.016) were less knowledgeable about food safety compared to their counterparts. Older consumers (36â45 years) were more knowledgeable about food safety compared to younger consumers (18â25 years) (B = 1.300, p < 0.011). Three-quarters of the respondents (76.9%) were not always confident about food safety issues when they bought SVFs, and affordability was the main reason (37.8%) for purchasing SVFs. Increased education and awareness on food safety education for Bangladeshi consumers of SVFs is needed, specifically targeting young adult males, and individuals with lower educational backgrounds
Synchronized Seed Germination and Seedling Growth of Black Cumin
Black cumin (Nigella sativa) is an important medicinal plant in the pharmacological industry. It is cultivated on a commercial scale, but its seeds have a slow, unsynchronized germination rate. Enhancing seed germination is crucial for improving the production of black cumin. The influence of presowing treatments [gibberellic acid (GA3), potassium nitrate, salicylic acid, and stratification at 4° C] on seed germination was assessed. Seed germination was determined daily for 30 days, and germination parameters, including final germination percentage (FGP), corrected germination rate, number of days to reach 50% of FGP, and seedling length vigor index, were evaluated. Endogenous contents of GA3 and abscisic acid (ABA) in nonstratified and stratified seeds were estimated using high-performance liquid chromatography (HPLC) and seedling growth was determined in 45-day-old seedlings. All presowing treatments tended to boost early germination for the first 10 days compared with the control. Low concentrations of GA3 at 0.25 g·L-1 also increased FGP (80%) compared with the control group (65.55%). Stratification for 4 weeks provided the greatest FGP value at 95.56%, and stratification for 3 weeks proved to be the most effective treatment for optimal seedling growth. Sodium dodecyl sulphateâpolyacrylamide gel electrophoresis patterns of stratified seeds revealed the alteration in intensities of 13 bands and the appearance of a new band (180 kDa) indicating a change in the synthesis of proteins during stratification. Moreover, stratification modulated the endogenous GA3 and ABA contents of black cumin seeds, which alleviated the physiological dormancy and resulted in high and synchronized seed germination
Nutritional potential of marine and freshwater algae as dietary supplements for growing rabbits
Influence of temperature and pH on the degradation of deoxynivalenol (DON) in aqueous medium: comparative cytotoxicity of DON and degraded product
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