Regulation of Fumarate Hydratase Gene Expression in Green Maize Leaves Under Hypoxic Conditions

The dynamics of fumarase activity in maize leaves under the conditions of different gas composition of the medium was studied. It is shown that under hypoxic conditions a decrease in this index of the enzyme under study is observed in comparison with normal conditions. It was found that in the environment of carbon dioxide and nitrogen two forms of the enzyme are observed, mitochondrial and cytoplasmic. The level of expression of fum1 and fum2 genes in green maize leaves was established in control and experimental samples. The dependence of the expression rate of fumarase genes in maize leaves is shown in different conditions of the gaseous medium

Regulation of Aconitate Hydratase Activity in Maize Leaves with Red and Blue Light

The dynamics of the activity of aconitate hydratase (AH, EC 4.2.1.3) in maize leaves under various light regimes was studied and it was established that red and blue light had an inhibitory effect on the functioning of the enzyme under study. Increased activity of AH in dark conditions is associated with activation of respiratory metabolism, as the main source of energy equivalents in the absence of light. The dependence of the expression rate of aco1 and aco2 genes in maize leaves on irradiation with light of different wavelengths is shown. The change in the expression rate of the aco1 gene, depending on the state of the phytochrome and cryptochromic systems, correlates with the activity of the encoded enzyme, indicating a phytochrome- and cryptochrome-mediated mechanism of regulation of the gene mediated. For the aco2 gene, the reverse dependence in phytochrome system regulation is shown