In vitro activity of Aloe extracts against Plasmodium falciparum

The antiplasmodial activity and toxicity data of 34 Aloe species and their main constituents were determined. A number of the methanol extracts possessed antimalarial activity where 50% of the parasite growth was inhibited (IC50 value) by 32 to 77μgml-1 of the extract. The chemical common to the most active species is the anthrone C-glucoside homonataloin which inhibited the chloroquine-resistant Plasmodium falciparum strain with an IC50 value of 13.46±1.36μgml-1. Homonataloin was a more potent inhibitor of parasite growth than aloin (IC50 value of 107.20±4.14μgml-1). The Aloe extracts tested did not exhibit any toxicity towards transformed human kidney epithelium cells at the concentrations (0.5-50μgml-1) used in the antimalarial assay

In vitro biological activities of South African Pelargonium (Geraniaceae) species

AbstractDespite commercial interest and ethnobotanical data, the pharmacological activities of a number of indigenous Pelargonium species hitherto remain poorly explored. The acetone extracts of twenty-one Pelargonium species (section Pelargonium) were included in this study. Using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, potent anti-oxidant activity was observed for the crude extracts of P. betulinum and P. crispum (IC50 values of 4.13±0.14 μg/ml and 4.49±0.18 μg/ml, respectively compared to ascorbic acid, IC50=4.72±0.14 μg/ml). The antimicrobial (both bacterial and fungal) potential of the extracts was evaluated by using the minimum inhibitory assay. The crude extracts of P. glutinosum (SBG), P. pseudoglutinosum, P. scabrum and P. sublignosum exhibited considerable antimicrobial activity against the Gram-positive test bacteria, with P. pseudoglutinosum demonstrating the highest inhibitory activity (MIC=39 μg/ml); however the aforementioned species were also relatively cytotoxic. The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) cellular viability assay was used to determine the toxicity of the acetone extracts against transformed human kidney epithelial (Graham) cells. The acetone extracts of P. sublignosum and P. citronellum (NBG) displayed the highest toxicities (IC50=11.89±1.54 μg/ml and 19.14±0.98 μg/ml, respectively). The results from the toxicity assay suggested that the antimicrobial activity of the extracts may be ascribed to general cytotoxic effects. Considering all the results collectively, Pelargonium cordifolium appears to be the most promising species requiring further investigation

PERBEDAAN TINGKAT PENGETAHUAN DAN SIKAP IBU HAMIL SEBELUM DAN SESUDAH PENYULUHAN MENGENAI IUD PASCAPLASENTA

Background : Post-placental IUD is defined as IUD insertion within 10 minutes after the expulsion of placenta. This method is considered safe and the most potential method to prevent missed opportunity. The use of post-placental IUD currently perceived incompatible with the expectation. One of the factors that cause this thing is the low knowledge level of post-placental IUD in society. Aim : To analyze the difference of knowledge level and attitude towards postplacental IUD on pregnant woman before and after counseling. Methods : This research was conducted using quasi experimental one group pretest-posttest design. Thirty-three samples were taken by consecutive sampling from pregnant woman who lived in the territory of Puskesmas Ngesrep and Puskemas Halmahera, Semarang. Data were analyzed using paired T-Test. Results : The mean value of knowledge level before counseling was 6,09 ± 1,826 whereas after counseling was 8,15 ± 1,661. The mean value of attitude before counseling was 35,79 ± 4,709 with 26 respondents had positive attitude and 7 respondents had negative attitude whereas after counseling the mean value was 39,97 ± 4,261 with all respondents had positive attitude. There was significant difference (p<0,05) on knowledge level (p=0,000) and attitude (p=0,000) before and after counseling. Conclusion : There was significant difference on knowledge level and attitude towards post-placental IUD on pregnant woman before and after counseling. Key words: Post-placental IUD, counseling, knowledge level, attitud

Phylogenomic analysis of a 55.1 kb 19-gene dataset resolves a monophyletic Fusarium that includes the Fusarium solani Species Complex

Scientific communication is facilitated by a data-driven, scientifically sound taxonomy that considers the end-user¿s needs and established successful practice. In 2013, the Fusarium community voiced near unanimous support for a concept of Fusarium that represented a clade comprising all agriculturally and clinically important Fusarium species, including the F. solani species complex (FSSC). Subsequently, this concept was challenged in 2015 by one research group who proposed dividing the genus Fusarium into seven genera, including the FSSC described as members of the genus Neocosmospora, with subsequent justification in 2018 based on claims that the 2013 concept of Fusarium is polyphyletic. Here, we test this claim and provide a phylogeny based on exonic nucleotide sequences of 19 orthologous protein-coding genes that strongly support the monophyly of Fusarium including the FSSC. We reassert the practical and scientific argument in support of a genus Fusarium that includes the FSSC and several other basal lineages, consistent with the longstanding use of this name among plant pathologists, medical mycologists, quarantine officials, regulatory agencies, students, and researchers with a stake in its taxonomy. In recognition of this monophyly, 40 species described as genus Neocosmospora were recombined in genus Fusarium, and nine others were renamed Fusarium. Here the global Fusarium community voices strong support for the inclusion of the FSSC in Fusarium, as it remains the best scientific, nomenclatural, and practical taxonomic option availabl

Die ontginning van die kommunikatiewe funksie van musiek in die erediens.

http://search.sabinet.co.za/WebZ/Authorize?sessionid=0&next=ej/ej_content_ngtt.html&bad=error/authofail.htm