Induction of population triploid-interploid in rainbow trout (Oncorhynchus mikiss) using by indirect

Induced polyploidy is a suitable tool for producing sterile fish which made commercial benefits in the aquaculture industry. This study carried out in order to produce triploid-interploid population via mating tetraploid female with diploid male rainbow trout. Heat shock was used for making tetraploid population and the best temperature and induction time were examined. Result showed that highest mortality from 1 day after fertilization to emerging were in groups 6 of embryos (40.1%) and the lowest were in groups 3 (33 %). Flowcytometry results showed that some fish were polyploidy. Comparative analyze of genome levels in tetraploid fish to control fish (diploid) and hen as standard indicator, confirmed tetraploid fishes in this study. In conclusion we can state that heat shock induction for 7 minutes at 65 hour –degree after fertilization in 28ºC is optimum temperature for inducting tetraploid rainbow trout

Increasing growth rate of Salmo trutta caspius by selection (Phase 1)

The management of genetically brood stocks of Salmo trutta of Caspian Sea in order to restocking for longe time harvesting and culture of fish in intensive system, has been little investigated, in contrast with European stocks. Salmo trutta Caspius is very important in biological and genetically point of view. This fish as gene bank valid for breeding improvement, hybridization and new cold species for culture, can be used. This study begain in 2007 and lasted 3 years in Bahonar, kelardasht of Iran. The breeders were 27 pairs. Each female have been crossed by 3 male. Eggs and larvae as well as the fingerlings reared separately of each crosses. The best fingerlings of 5, 20 and 100 gram were collected after 3 period of challenge. At the final 300 brood stocks in two groups were remained and stocked for artificial propagation. For heritability estimation in 4 stages sampling the weight and length were measured and it was 0.47 and 0.50 respectively. In case of molecular study the quality of DNA extract from the fin of breeders and their progeny by phenol-chloroform was good for PCR test. The heterosigosity test for Ho and He for each station and sites except for SRUTTA12 and STRUTTA59, the He was greater than Ho. Shaton Index and Hardy-Weinberg equilibrium for station and sites for male and progenies, the OM24showed deviation from H-W equilibrium. In case of similarity and genetic distance based on Nei (1972), the highest distance were in case of male and female, and were the least between fingerlings and male brood stocks

Determination of suitable age and size for releasing of Salmo trutta caspius by evaluation of osmotic

This study was carried out to determine the appropriate size of Caspian trout (Salmo trutta caspius Kessler, 1877) juveniles for releasing to South Caspian Sea or possibility of cage culture in Caspian Sea water. 1611 specimens were exposed in 4 weight groups of 5, 10, 15 and 20 g, in 3 salinity trials: Caspian Water (11- 11.5), inshore water (7) and fresh water (control). Each trial was done in 3 replicates. The blood samples and tissue fixations carried out from juveniles of control group (in fresh water) and 3, 6, 12, 24, 72, 168, 240 hours after exposure of fish in different treatments. Plasma osmolal ity, Na^+ and Cl^- concentrations, were measured by osmometer, flame photometer, RA1000 respectively. Plasma cortisol level was determined by using RIA (radio immunochemical assay). Na^+, K^+-ATPase activity in homogenates of gills was estimated by phosphate released from ATP. Histological indicators including chloride cell diameter and nephron morphometric parameters were assessed using classic preparation and optic microscope with digital camera. Results of osmolality and ions measuring concurrently show that all weight groups can live in salinity of 7 and they maintain the osmolality and ion concentrations. In the Caspian water, weight groups excluding 5 g juveniles show same result. Mean plasma osmolality of 20, 15, 10 and 5 gr juveniles in control group (time of 0) were calculated 331.3±8.7, 307.7±6, 334.7±14.6 and 301±8.7 mosml/l. This parameter in the above weight groups after 240 hours exposure in the Caspian Sea water were measured 329±0.53, 321±9, 325.3±6.7 and 346.5±13.6 respectively. The observation of kidney glomeruli in histological sections shows that the diameter of glomeruli in 5, 10, 15 g weight groups in 7 and all groups in Caspian water decreased after 72 h adaptation period (p0.05) for 5g juveniles, whereas within weight groups of 10, 15 and 20 g in Caspian Sea water and groups of 15, 20 g in water of 7 salinity, the increase (p0.05), although in other groups, a significant increase of this parameter was detected during experiments. Na^+ ,K^+ -ATPase activity in juveniles of 5g weight group in 7 salinity and Caspian water was low (3.2 6.1 mol Pi /mg protein/ h). The enzyme activities in all weight groups were higher under the exposure in Caspian Sea water than that in water of 7 salinity. In group of 10 g juveniles at start time (control in freshwater) the activity of Na^+, K^+ -ATPase was significantly higher (p<0.05) than that in 20g group. It is may be related to some metabolic changes and transforming to parr-smolt

Evaluating of the results of long fusion surgery in patients with degenerative lumbar scoliosis

Background: Degenerative scoliosis most commonly affects the lumbar spine in the elderly, resulting in facet and disc degeneration, leading to increased pain and progressive deformity. Due to the importance of the results of long fusion and the rate of coronary and sagittal correction of imbalance in patients with degenerative scoliosis, the present study was performed to evaluate the results of long fusion surgery in patients with degenerative lumbar scoliosis. Methods: The present study was performed as a prospective cohort study in patients with degenerative scoliosis who were candidates for surgery at Al-Zahra Hospital in Isfahan. Basic patient information including age, sex, anatomical location of vertebral deviation and slip, degree of severity of deviation based on cobb angle and number of previous surgeries were reviewed and recorded. The surgical characteristics of the patient were extracted from the medical record including the approach used, the anatomical location of the fusion, whether or not to perform decompression, the number of decompression and fusion levels, the amount of bleeding during surgery, and the duration of surgery. Results: Eleven female patients participated in this study. The mean age of patients was 55.64 years with a standard deviation of 7.67 years. The minimum age was 40 years and the maximum age was 66 years. Patients' symptoms included low back pain-leg pain (3 cases), back pain-lameness (2 cases) and back pain-leg pain-lameness (6 cases). All patients underwent two stages of surgery. Mean amount of bleeding and time of surgery in the second surgery were significantly reduced compared to the first surgery (p <0.05). Complications of surgery included proximal junctional vertebral fracture (PGK) in two patients. Conclusion: Degenerative scoliosis is a complex clinical condition in which the patient's main problems are pain and disability. Long fusion surgery in patients with degenerative scoliosis significantly reduced the mean deviation, Patient's pain and severity of disability 6 months after surgery. Copyright © 2021 Tehran University of Medical Sciences. Published by Tehran University of Medical Sciences

Revision procedures after initial Roux-en-Y gastric bypass, treatment of weight regain: a systematic review and meta-analysis

Morbid obesity is a global chronic disease, and bariatric procedures have been approved as the best method to control obesity. Roux-en-Y gastric bypass is one of the most common bariatric surgeries in the world and has become the gold standard procedure for many years. However, some patients experience weight regain or weight loss failure after the initial bypass surgery and require revisional or conversional interventions. International databases including PubMed, International Scientific Indexing (ISI), and Scopus were considered for a systematic search of articles that were published by 5th of May 2020. Forty-one published studies, which reported revision procedure on 1403 patients, were selected and analyzed for this review. The selected studies were categorized into six groups of revision procedures, including laparoscopic pouch resizing and/or revision of gastro-jejunal anastomosis (GJA), adjustable or non-adjustable gastric band over pouch ± pouch/GJA resizing, endoscopic revision of gastric GJA ± pouch, distal Roux-en-Y gastric bypass (DRGB), biliopancreatic diversion with duodenal switch (BPD-DS) or single anastomosis duodeno-ileal bypass with gastric sleeve (SADI-S), DRGB + Band or pouch/GJA resizing. Revision procedures result in more weight loss after the initial weight loss procedures. In the one-year follow-up, DRGB by itself with standardized mean difference (SMD) of � 1.24 presented a greater decrease in body mass index (BMI). DRGB plus band or pouch/GJA resizing, BPD-DS or SADI, adjustable or non-adjustable gastric banding over pouch ± pouch/GJA resizing, endoscopic pouch and/or GJA revision and laparoscopic pouch or/and GJA resizing revealed a lower decrease in BMI in order, respectively. In the three-year follow-up, BPD-DS or SADI-S method with SMD of � 1.40 presented the highest decrease in BMI. In follow, DRGB alone, adjustable or non-adjustable gastric banding over gastric pouch ± pouch / GJA resizing, DRGB + Band or gastric pouch/GJA resizing, laparoscopic pouch and/or GJA resizing and endoscopic revision of pouch and/or GJA revealed less reduction in BMI, respectively. In the five-year follow-up, DRGB alone procedures with SMD of � 2.17 presented the greatest reduction in BMI. Subsequently, BPD-DS or SADI-S, laparoscopic pouch and/or GJA size revision, and endoscopic revision of GJA/pouch revealed less overall decrease in BMI in order. All methods of revision procedures after the initial RYGB have been effective in the resolution of weight regain. However, based on the findings in this systematic review, it seems DRGB or BPD-DS/SADI-S is the most effective procedure in the long-term follow-up outcome. More studies with a higher number of patients and even longer follow-ups will be required to obtain more accurate data and outcome. © 2021, Italian Society of Surgery (SIC)

Revision procedures after initial Roux-en-Y gastric bypass, treatment of weight regain: a systematic review and meta-analysis

Morbid obesity is a global chronic disease, and bariatric procedures have been approved as the best method to control obesity. Roux-en-Y gastric bypass is one of the most common bariatric surgeries in the world and has become the gold standard procedure for many years. However, some patients experience weight regain or weight loss failure after the initial bypass surgery and require revisional or conversional interventions. International databases including PubMed, International Scientific Indexing (ISI), and Scopus were considered for a systematic search of articles that were published by 5th of May 2020. Forty-one published studies, which reported revision procedure on 1403 patients, were selected and analyzed for this review. The selected studies were categorized into six groups of revision procedures, including laparoscopic pouch resizing and/or revision of gastro-jejunal anastomosis (GJA), adjustable or non-adjustable gastric band over pouch ± pouch/GJA resizing, endoscopic revision of gastric GJA ± pouch, distal Roux-en-Y gastric bypass (DRGB), biliopancreatic diversion with duodenal switch (BPD-DS) or single anastomosis duodeno-ileal bypass with gastric sleeve (SADI-S), DRGB + Band or pouch/GJA resizing. Revision procedures result in more weight loss after the initial weight loss procedures. In the one-year follow-up, DRGB by itself with standardized mean difference (SMD) of � 1.24 presented a greater decrease in body mass index (BMI). DRGB plus band or pouch/GJA resizing, BPD-DS or SADI, adjustable or non-adjustable gastric banding over pouch ± pouch/GJA resizing, endoscopic pouch and/or GJA revision and laparoscopic pouch or/and GJA resizing revealed a lower decrease in BMI in order, respectively. In the three-year follow-up, BPD-DS or SADI-S method with SMD of � 1.40 presented the highest decrease in BMI. In follow, DRGB alone, adjustable or non-adjustable gastric banding over gastric pouch ± pouch / GJA resizing, DRGB + Band or gastric pouch/GJA resizing, laparoscopic pouch and/or GJA resizing and endoscopic revision of pouch and/or GJA revealed less reduction in BMI, respectively. In the five-year follow-up, DRGB alone procedures with SMD of � 2.17 presented the greatest reduction in BMI. Subsequently, BPD-DS or SADI-S, laparoscopic pouch and/or GJA size revision, and endoscopic revision of GJA/pouch revealed less overall decrease in BMI in order. All methods of revision procedures after the initial RYGB have been effective in the resolution of weight regain. However, based on the findings in this systematic review, it seems DRGB or BPD-DS/SADI-S is the most effective procedure in the long-term follow-up outcome. More studies with a higher number of patients and even longer follow-ups will be required to obtain more accurate data and outcome. © 2021, Italian Society of Surgery (SIC)

Inhibitor development in patients with congenital factor VII deficiency, a study on 50 Iranian patients

: Congenital factor VII (FVII) deficiency is a rare bleeding disorder with an estimated prevalence of 1 per 500�000 in the general population. On-demand replacement therapy is the main therapeutic choice in patients with congenital FVII deficiency. Inhibitor formation against exogenous FVII is very rare and can cause challenges in the management of the disorder. The present study was conducted to assess the prevalence of FVII inhibitor in 50 patients with congenital FVII deficiency under on-demand or prophylaxis treatment by recombinant activated FVII. All patients with confirmed congenital FVII deficiency were assessed for inhibitor development in regular intervals. Inhibitor titer was determined by a modified Nijmegen-Bethesda assay. The study results were analyzed by SPSS software. Among all cases, two patients (4) developed an FVII inhibitor. Case 1 was a 14-year-old boy with severe FVII deficiency (FVII activity <1) with regular prophylaxis. The patient was a high-responder with high-titer FVII inhibitor (170�Bethesda Unit). This patient, who had a history of intracranial hemorrhage, had undergone brain surgery three times. The second patient was a 70-years old man with on-demand therapy that also developed a high-titer inhibitor (10�Bethesda Unit). This patient had experienced easy bruising and endured a few surgeries for his brain tumor and, finally, succumbed to the disease. Although the inhibitor formation is a rare phenomenon, it may result in a significant challenge to manage the affected patients