40 research outputs found
Active region formation through the negative effective magnetic pressure instability
The negative effective magnetic pressure instability operates on scales
encompassing many turbulent eddies and is here discussed in connection with the
formation of active regions near the surface layers of the Sun. This
instability is related to the negative contribution of turbulence to the mean
magnetic pressure that causes the formation of large-scale magnetic structures.
For an isothermal layer, direct numerical simulations and mean-field
simulations of this phenomenon are shown to agree in many details in that their
onset occurs at the same depth. This depth increases with increasing field
strength, such that the maximum growth rate of this instability is independent
of the field strength, provided the magnetic structures are fully contained
within the domain. A linear stability analysis is shown to support this
finding. The instability also leads to a redistribution of turbulent intensity
and gas pressure that could provide direct observational signatures.Comment: 19 pages, 10 figures, submitted to Solar Physic
Mouse Chromosome 11
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46996/1/335_2004_Article_BF00648429.pd
Selection for tuberosum likeness in the vines and in the tubers in a population of Neotuberosum
Derivatives of a benzoquinone acyl hydrazone with activity against Toxoplasma gondii
Toxoplasma gondii is an obligate intracellular parasite with global incidence. The acute infection, toxoplasmosis, is treatable but current regimens have poor host tolerance and no cure has been found for latent infections. This work builds upon a previous high throughput screen which identified benzoquinone acyl hydrazone (KG8) as the most promising compound; KG8 displayed potent in vitro activity against T. gondii but only marginal in vivo efficacy in a T. gondii animal model. To define the potential of this new lead compound, we now describe a baseline structure-activity relationship for this chemotype. Several derivatives displayed IC50's comparable to that of the control treatment pyrimethamine with little to no cytotoxicity. The best of these, KGW44 and KGW59, had higher metabolic stability than KG8. In an in vivo T. gondii murine model, KGW59 significantly increased survivorship. This work provides new insights for optimization of this novel chemotype. Keywords: Toxoplasma gondii, Drug discovery, Lead compounds, Anti-parasitic
Physiology, metabolism, and fossilization of hot-spring filamentous microbial mats
The evolutionarily ancient Aquificales bacterium Sulfurihydrogenibium spp. dominates filamentous microbial mat communities in shallow, fast-flowing, and dysoxic hot-spring drainage systems around the world. In the present study, field observations of these fettuccini-like microbial mats at Mammoth Hot Springs in Yellowstone National Park are integrated with geology, geochemistry, hydrology, microscopy, and multi-omic molecular biology analyses. Strategic sampling of living filamentous mats along with the hot-spring CaCO3 (travertine) in which they are actively being entombed and fossilized has permitted the first direct linkage of Sulfurihydrogenibium spp. physiology and metabolism with the formation of distinct travertine streamer microbial biomarkers. Results indicate that, during chemoautotrophy and CO2 carbon fixation, the 87-98% Sulfurihydrogenibium-dominated mats utilize chaperons to facilitate enzyme stability and function. High-abundance transcripts and proteins for type IV pili and extracellular polymeric substances (EPSs) are consistent with their strong mucus-rich filaments tens of centimeters long that withstand hydrodynamic shear as they become encrusted by more than 5âmm of travertine per day. Their primary energy source is the oxidation of reduced sulfur (e.g., sulfide, sulfur, or thiosulfate) and the simultaneous uptake of extremely low concentrations of dissolved O2 facilitated by bd-type cytochromes. The formation of elevated travertine ridges permits the Sulfurihydrogenibium-dominated mats to create a shallow platform from which to access low levels of dissolved oxygen at the virtual exclusion of other microorganisms. These ridged travertine streamer microbial biomarkers are well preserved and create a robust fossil record of microbial physiological and metabolic activities in modern and ancient hot-spring ecosystems.Yiran Dong, Robert A. Sanford, William P. Inskeep, Vaibhav Srivastava, Vincent Bulone, Christopher J. Fields, Peter M. Yau, Mayandi Sivaguru, Dag AhrĂ©n, Kyle W. Fouke, Joseph Weber, Charles R. Werth, Isaac K. Cann, Kathleen M. Keating, Radhika S. Khetani, Alvaro G. Hernandez, Chris Wright, Mark Band, Brian S. Imai, Glenn A. Fried, and Bruce W. Fouk