DETECTION OF ADENOVIRUS ANTIGEN BY A SURFACE-ENHANCED RAMAN SCATTERING ENZYME-LINKED IMMUNOSORBENT ASSAY

Aim. Study of the possibility of adenovirus antigen detection by recording of surface-enhanced Raman scattering (SERS) spectra of enzyme oxidized product of 3,3',5,5'-tetramethylbenzidine. Materials and methods. Clinical fecal samples containing adenoviruses, group A rotaviruses, noroviruses and healthy children samples, as well as laboratory strains of adenoviruses with a titer of 5 — 6 lg TCD50/ml were used. Sandwich immunoassay was used, the Raman spectra were recorded by a Raman spectrometer (532 nm) after incubation with silver nanoparticles. Results. The concordance of the adenovirus detection results was obtained in comparison with the enzyme immunoassay method with colorimetric detection and PCR. Conclusion. The possibility of TMB+ using as a SERS reporter and silver nanoparticles as a SERS substrate for the detection of adenovirus antigen in complex biological samples was shown

Invasion ecology: an international perspective centered in the Holarctic

The Fourth International Symposium on Alien Species in the Holarctic was convened September 22-28, 2013, by the Russian Academy of Sciences at the I.D. Papanin Institute for the Biology of Inland Waters (IBIW) on the Volga River in Borok (approximate to 355 km north of Moscow). The Organizing Committee spanned five countries (France, People's Republic of China, Poland, Russian Federation, and the United States), with participants (n=150) across the breadth and depth of the Russian Federation, from countries in proximity to it (i.e., Armenia, Lithuania, Kazakhstan, Kyrgyzstan, Ukraine, Siberia, and The Republic of China), and more globally from the United States to Tasmania and Norway to South Africa. This report provides a synopsis of invasive species issues that were discussed at the symposium and, as such, provides an international window for the evaluation of fisheries-related topics in this part of the globe