Isolation of GAL Tularemia Bacteriophage and its Characteristics

Temperate tularemia bacteriophage was for the first time isolated from the organs of guinea-pig infected with live tularemia vaccine strain N 15 of RIEH line. Negative colonies of bacteriophage were up to 0.2 mm in diameter with incomplete lysis zone at the periphery. In view of the results of electronic microscopy bacteriophage represented filamentous carpuscules. Bacteriophage lyzed bacteria of three subtypes of tularemia etiological agent and bacteria of the main species of legionellosis etiological agents. The simple use of bacteriophage allows to recommend new tularemia bacteriophage GAL for practical application

Development of Methodic Approach to Construction of Recombinant Strain, Producer of the Cholera Toxin B Subunit

Presented are the results of experiments on the construction of Escherichia coli Top10 (pTrcSuB) strain, perspective for the development of recombinant cholera vaccine for peroral use. The obtained strain possesses inducible expression of cholera toxin B subunit. Peculiarities of cloned gene expression in strain-producer and the conditions of its maintaining were studied. The proposed approach will allow to obtain effective and safe cholera vaccines