EVALUATION OF VIBRIO CHOLERAE 569B INABA PROTECTIVE ANTIGENES, DERIVED ON INDUSTRIAL AND DEVELOPED BIOREACTORS AS WELL AS BY IMPROVED TECHNOLOGY

We evaluated immunochemical, physical and biochemical properties of Vibrio cholerae 569B INABA protective antigenes, derived on industrial and own-developed bioreactors as well as by technology of its concentration by tangential ultrafiltration. We detected, protease, twinase and. lysophospholipase in all samples. Also, dotimmunoanalysis showed equal concentration, of cholerogen-anatoxine and. O-antigen in all samples too. Using chromatography and. electrophoresis, we found their properties as similar. Thus, we suppose to be possible using developed bioreactor as well as technology of Vibrio cholerae 569B INABA protective antigens concentration by tangential concentration during a process of synthetic oral cholera vaccine production

ISOLATION OF GLICOPROTEID FROM THE FIXED RABIES VIRUS, STRAIN «MOSCOW 3253», AND CONSTRUCTING OF DOT-IMMUNOASSAY DIAGNOSTICUM ON ITS BASIS

Described here are the results of glicoproteid isolation from the fixed rabies virus, strain «Moscow 3253», using non-ionic detergent with subsequent chromatographic purification. The obtained antigen was demonstrated to be applicable as immunoreagent for construction of diagnosticum, by means of conjugation with colloid gold nanoparticles. The diagnosticum is meant for detection of specific antibodies in immune sera of horsesproducers, and in the preparation of anti-rabies immunoglobulin, in dot-immunoassay